To defend against foreign pathogens, plants and animals employ proteins known as immune sensors which recognize extracellular molecules and initiate immune response. Despite playing a critical in innate immunity, the underlying mechanisms governing this recognition process have remained elusive.
Previous work by the team has shown that in plants, HSP90, a molecular chaperone, binds to the proteins SGT1 and RAR1 to form a complex which mediates the activity of immune sensors, but the dynamic nature of the complex has prevented a detailed understanding of its structure and role.
The researchers applied a variety of analysis techniques to overcome this challenge, each targeting different aspects of the RAR1-SGT1-HSP90 complex. Biochemical analysis identified the CHORD2 domain of RAR1 and the CS domain of SGT1 as essential to formation of the complex, while crystal structure analysis revealed that the complex is made up of six proteins in a ring configuration, with two copies of each component. Functionally, the team identified RAR1-SGT1 interaction as playing a key role in disease resistance, also providing evidence that interaction of RAR1 and HSP90 increases decomposition of ATP and boosts the efficiency of immune sensor control.
Reported in the July 30 issue of Molecular Cell, these findings provide the first detailed framework for understanding the architecture of HSP90-SGT1-CHORD protein complexes, whose role in immune defense is shared in both plants and animals. Future applications in agriculture and health promise increased crop yields through disease resistance and new treatments for human diseases.
For more information, please contact:Dr. Ken Shirasu
The RIKEN Plant Science Center (PSC), located at the RIKEN Yokohama Research Institute in Yokohama City, Japan, is at the forefront of research efforts to uncover mechanisms underlying plant metabolism, morphology and development, and apply these findings to improving plant production. With laboratories ranging in subject area from metabolomics, to functional genomics, to plant regulation and productivity, to plant evolution and adaptation, the PSC’s broad scope grants it a unique position in the network of modern plant science research. In cooperation with universities, research institutes and industry, the PSC is working to ensure a stable supply of food, materials, and energy to support a growing world population and its pressing health and environmental needs.
Multi-institutional collaboration uncovers how molecular machines assemble
02.12.2016 | Salk Institute
Fertilized egg cells trigger and monitor loss of sperm’s epigenetic memory
02.12.2016 | IMBA - Institut für Molekulare Biotechnologie der Österreichischen Akademie der Wissenschaften GmbH
A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.
Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...
In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.
“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...
The Max Planck Institute for Physics (MPP) is opening up a new research field. A workshop from November 21 - 22, 2016 will mark the start of activities for an innovative axion experiment. Axions are still only purely hypothetical particles. Their detection could solve two fundamental problems in particle physics: What dark matter consists of and why it has not yet been possible to directly observe a CP violation for the strong interaction.
The “MADMAX” project is the MPP’s commitment to axion research. Axions are so far only a theoretical prediction and are difficult to detect: on the one hand,...
Broadband rotational spectroscopy unravels structural reshaping of isolated molecules in the gas phase to accommodate water
In two recent publications in the Journal of Chemical Physics and in the Journal of Physical Chemistry Letters, researchers around Melanie Schnell from the Max...
The efficiency of power electronic systems is not solely dependent on electrical efficiency but also on weight, for example, in mobile systems. When the weight of relevant components and devices in airplanes, for instance, is reduced, fuel savings can be achieved and correspondingly greenhouse gas emissions decreased. New materials and components based on gallium nitride (GaN) can help to reduce weight and increase the efficiency. With these new materials, power electronic switches can be operated at higher switching frequency, resulting in higher power density and lower material costs.
Researchers at the Fraunhofer Institute for Solar Energy Systems ISE together with partners have investigated how these materials can be used to make power...
16.11.2016 | Event News
01.11.2016 | Event News
14.10.2016 | Event News
02.12.2016 | Medical Engineering
02.12.2016 | Agricultural and Forestry Science
02.12.2016 | Physics and Astronomy