Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Progress in Stem Cell Research based on SLOT

07.10.2013
The Laser Zentrum Hannover (LZH e.V.) is currently active in a project dealing with the tomographic monitoring of 3-D cell cultures consisting of pluripotent stem cells (hPSC).

The goal of the collaborative research project is, for the first time, to use Scanning Laser Optical Tomography (SLOT) for non-invasive, direct, quantitative compilation of the absolute number of cells in cultured, endogenous cell groups.


Image of an uncolored aggregate / spheroids from hiPS cells using intrinsic contrast mechanisms with SLOT: Raw data for scattered light (top left) and extinction (middle left) and superposition of both channels (lower left), reconstructed data after filtering the rear projection equivalent to the raw data, rendered image of the superposition of both channels, volume image (top right), in silico cross-section of a 50 µm cut (middle right), and a 15 µm cut (lower right), (the scale bars depict 100 µm).

The results of the project TOMOSphere should bring a better understanding of the physiology of hPSC and other stem cells, as well as a continuous control of their characteristics, making decisive progress in therapeutic concepts possible.

For the investigations, SLOT-technology, which was developed and patented at the LZH, was used for the first time for the temporally and spatially resolved observation of native or fixed cells in three-dimensional aggregate structures. Knowledge gained from using this process can be used to classify and later to sort the aggregates, for example concerning heterogeneity of the cell structure, possible cyst formation, or the deposition of extracellular matrixes.

Furthermore, conclusions concerning important process parameters for the cultivation of aggregates in stirred suspension cultures can be made, such as inoculation densities, the influence of the culture medium, or the maximum or optimal cell and aggregate density per ml of culture medium.

In order to gather this information, the collaborative project is developing an incubation system based on SLOT for tomographic long-term investigations of tissue samples, combined with a wide spectrum of contrast methods. On the one hand, this process should enable marker-free identification of intrinsic cell and tissue specific characteristics, and on the other hand, provide a secondary contrast method using low molecular substances.

For example, with this method, the fluctuation of NAD/NADH, cAMP, Ca2+ ions and their enrichments can be observed, or various states of cell cycles up to a programmed cell death can be verified. Further, it can be used to observe and analyze differential intra- or extracellular agglomerations, or to image different micro- and nanoparticles in cell aggregates.

By using SLOT based, marker-free verification methods for stem cell pluripotency, a higher sensitivity can be reached, especially in comparison to conventional methods. From a technical point of view, a cuvette with cell aggregates is scanned using a needle beam, and a projection image for each scanning position is made from the scattered light, the transmitted light and the fluorescent light. While turning the sample, projections are recorded, and then based on a back projection algorithm, a 3-D data set is generated.

In silico slices can be generated this way, which enable a view deep into the sample material in absence of performing classical histology. Basically, this method can be used to image any isolated and sufficiently transparent sample.

In order to carry out process research and development and controlling on a larger scale, an industrially relevant bioreactor platform with integrated SLOT technology for the mass production of human pluripotent stem cells, and based on this differentiation of cell types, should appear on the market.

Cells resulting from this setup can be used in regenerative medicine, for in vitro modeling of human diseases and illnesses, and for new methods for therapeutic approaches, or for the development of pharmacologically active substances.

The project with a total funding volume of 4.134 Mio. € is part of the “Ultrasensitive Verification and Manipulation of Cells and/or Tissue and their molecular Substances” call for proposals of the German Ministry of Education and Research (BMBF). Apart from the LZH, there are five other partners in the collaborative project (LaVision BioTec/Bielefeld, LEBAO/Hannover, Scivis/Göttingen, Sill Optics/Wendelstein and Miltenyi BioTec/Bergisch Gladbach).

The project started on July 1st, 2013, and the consortium will be funded in the next three years by the BMBF with a total of 2.621 Mio. €.

Contact:
Laser Zentrum Hannover e.V. (LZH)
Lena Bennefeld
Hollerithallee 8
D-30419 Hannover
Tel.: +49 511 2788-238
Fax: +49 511 2788-100
E-Mail: l.bennefeld@lzh.de
The Laser Zentrum Hannover e.V. (LZH) carries out research and development in the field of laser technology and is supported by the Ministry of Economic Affairs, Labour and Transport of the State of Lower Saxony (Niedersächsisches Ministerium für Wirtschaft, Arbeit und Verkehr).

Michael Botts | Laser Zentrum Hannover e.V.
Further information:
http://www.lzh.de

More articles from Life Sciences:

nachricht Could this protein protect people against coronary artery disease?
17.11.2017 | University of North Carolina Health Care

nachricht Microbial resident enables beetles to feed on a leafy diet
17.11.2017 | Max-Planck-Institut für chemische Ökologie

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: A “cosmic snake” reveals the structure of remote galaxies

The formation of stars in distant galaxies is still largely unexplored. For the first time, astron-omers at the University of Geneva have now been able to closely observe a star system six billion light-years away. In doing so, they are confirming earlier simulations made by the University of Zurich. One special effect is made possible by the multiple reflections of images that run through the cosmos like a snake.

Today, astronomers have a pretty accurate idea of how stars were formed in the recent cosmic past. But do these laws also apply to older galaxies? For around a...

Im Focus: Visual intelligence is not the same as IQ

Just because someone is smart and well-motivated doesn't mean he or she can learn the visual skills needed to excel at tasks like matching fingerprints, interpreting medical X-rays, keeping track of aircraft on radar displays or forensic face matching.

That is the implication of a new study which shows for the first time that there is a broad range of differences in people's visual ability and that these...

Im Focus: Novel Nano-CT device creates high-resolution 3D-X-rays of tiny velvet worm legs

Computer Tomography (CT) is a standard procedure in hospitals, but so far, the technology has not been suitable for imaging extremely small objects. In PNAS, a team from the Technical University of Munich (TUM) describes a Nano-CT device that creates three-dimensional x-ray images at resolutions up to 100 nanometers. The first test application: Together with colleagues from the University of Kassel and Helmholtz-Zentrum Geesthacht the researchers analyzed the locomotory system of a velvet worm.

During a CT analysis, the object under investigation is x-rayed and a detector measures the respective amount of radiation absorbed from various angles....

Im Focus: Researchers Develop Data Bus for Quantum Computer

The quantum world is fragile; error correction codes are needed to protect the information stored in a quantum object from the deteriorating effects of noise. Quantum physicists in Innsbruck have developed a protocol to pass quantum information between differently encoded building blocks of a future quantum computer, such as processors and memories. Scientists may use this protocol in the future to build a data bus for quantum computers. The researchers have published their work in the journal Nature Communications.

Future quantum computers will be able to solve problems where conventional computers fail today. We are still far away from any large-scale implementation,...

Im Focus: Wrinkles give heat a jolt in pillared graphene

Rice University researchers test 3-D carbon nanostructures' thermal transport abilities

Pillared graphene would transfer heat better if the theoretical material had a few asymmetric junctions that caused wrinkles, according to Rice University...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Ecology Across Borders: International conference brings together 1,500 ecologists

15.11.2017 | Event News

Road into laboratory: Users discuss biaxial fatigue-testing for car and truck wheel

15.11.2017 | Event News

#Berlin5GWeek: The right network for Industry 4.0

30.10.2017 | Event News

 
Latest News

NASA detects solar flare pulses at Sun and Earth

17.11.2017 | Physics and Astronomy

NIST scientists discover how to switch liver cancer cell growth from 2-D to 3-D structures

17.11.2017 | Health and Medicine

The importance of biodiversity in forests could increase due to climate change

17.11.2017 | Studies and Analyses

VideoLinks
B2B-VideoLinks
More VideoLinks >>>