Known to the public for their use in yogurt and other foods to improve human digestion and health, probiotic bacteria isolated from other sources can also be used to improve survival, nutrition and disease prevention in larvae grown in shellfish hatcheries.
Researchers at NOAA’s Milford Laboratory in Milford, Conn. have shown that naturally-occurring bacteria isolated from the digestive glands of adult eastern oysters (Crassostrea virginica) and northern bay scallops (Argopecten irradians irradians) may be used as potential probiotic candidates in oyster larviculture.
Two related research studies published in the Journal of Shellfish Research identify a new probiotic bacterium, designated OY15, which has been shown to significantly improve larval survival in pilot-scale trials during the first two weeks of life, the most critical stage for the organism when mortality rates are among the highest.
“We are cautiously optimistic that this probiotic candidate, OY15, will offer a number of significant benefits to the shellfish industry,” said Gary Wikfors, co-author of both studies and head of the Milford Laboratory’s Biotechnology Branch. “Commercial and public shellfish hatcheries can have low survival rates for shellfish seed during the first two weeks, so improving those survival rates and the health of the organisms beyond that point is a pretty significant step forward.”
Hatcheries produce shellfish seed to supplement natural seed, which is often limited by loss of habitat, contamination from pollution, climate change and other factors. Bacterial diseases caused mainly by pathogenic bacteria such as Vibrio are a major cause of mortality in hatchery shellfish, particularly at the very early larval stage. The result: significant financial losses to commercial growers and to production of farmed shellfish, which accounts for 25 percent of the total world aquaculture product.
Antimicrobial drugs approved for use in aquaculture in some countries, but not the US, have traditionally been used to treat bacterial diseases, but overuse of antibiotics can result in the development of resistant strains of bacterial pathogens. The use of probiotic bacteria has become increasingly popular for improved nutrition, healthy digestion and disease prevention and is used in human foods like yogurt and in pet foods.
As demand for environmentally-friendly aquaculture grows, the use of probiotics for disease prevention and improved nutrition in shellfish aquaculture is also growing. While a number of research studies have shown promise, development of probiotics that can be used in aquaculture is a multistep process requiring fundamental research and full-scale trials.
“The objective of the first part of this study was to isolate and evaluate new probiotic bacteria which, when incorporated into foods used in shellfish hatcheries, might significantly improve larval survival,” said co-author Diane Kapareiko, a microbiologist at the Milford Laboratory. The second part of the study was to test the new probiotic candidate on the survival of oyster larvae in pilot-scale trials during their first two weeks of life.
“We conducted a very cautious, step by step study, to identify the best candidates under a variety of scenarios," Wikfors said. "Our bench-scale challenge studies indicated that oyster larvae exposed to probiotic candidate OY15 had the highest survival rate, and that the survival of pathogen-challenged larvae was further improved by the presence of OY 15 compared to the pathogen alone. It is somewhat analogous to a human building up immunity to a certain organism by being exposed to it, but without the involvement of antibodies.”
The Milford scientists isolated 26 candidate probiotic bacteria from oysters and scallops of which 16 had an inhibitory effect against a known shellfish-larval pathogen (B183) of the Vibrio species of bacteria. Further screening for safe use in culturing the oyster larvae and their microalgal feed indicated which probiotic candidates would inhibit growth of the pathogen most effectively and therefore could confer a protective effect upon oyster larval survival.
Lab studies indicated that survival of two-day old oyster larvae during two-week pilot scale trials improved when supplemented with the probiotic candidate OY15 strain. Four treatments were conducted: a larval control with no bacteria, a pathogen control with larvae and pathogen B183 only, a probiotic control with larvae and probiotic candidate OY15 only, and a combination treatment comprised of larvae and both probiotic and pathogen.
“Our research focused on the critical first stage of larval growth, when mortality rates are among the highest,” Kapareiko said. Positive effects of probiotic candidate OY15 were found on the survival of oyster larvae (short term), on growth of phytoplankton used as larval feed, and upon oyster survival during pilot-scale larviculture conditions.
“This two-part study confirms that use of naturally-occurring probiotic bacteria confers protection to oyster larvae against bacterial disease and improves their survival,” Kapareiko said. “The results can be used as guidelines for isolating and screening other potential probiotic candidates for similar aquaculture applications, and provide the basis for developing functional foods for use in shellfish hatcheries that incorporate a naturally occurring, probiotic bacteria.”
NOAA Fisheries Service is dedicated to protecting and preserving our nation’s living marine resources and their habitat through scientific research, management and enforcement. NOAA Fisheries Service provides effective stewardship of these resources for the benefit of the nation, supporting coastal communities that depend upon them, and helping to provide safe and healthy seafood to consumers and recreational opportunities for the American public.
NOAA’s mission is to understand and predict changes in the Earth's environment, from the depths of the ocean to the surface of the sun, and to conserve and manage our coastal and marine resources. Join us on Facebook, Twitter and our other social media channels.
Shelley Dawicki | EurekAlert!
A novel socio-ecological approach helps identifying suitable wolf habitats
17.02.2017 | Universität Zürich
New, ultra-flexible probes form reliable, scar-free integration with the brain
16.02.2017 | University of Texas at Austin
In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport
Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...
The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.
The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...
Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...
Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".
Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...
13.02.2017 | Event News
10.02.2017 | Event News
09.02.2017 | Event News
17.02.2017 | Medical Engineering
17.02.2017 | Medical Engineering
17.02.2017 | Health and Medicine