DNA is not merely a carrier of genetic information; DNA is a useful building material for nanoscale structures. In a way similar to origami, the Japanese art of paper folding, a long single strand of DNA can be folded into nearly any three-dimensional shape desired with the use of short DNA fragments.
Various methods for binding proteins to DNA-origami structures have previously been developed, but in most cases they require modification of the protein. “A method based only on proteins is desirable,” says Morii, “because it would simplify and accelerate the binding of proteins to the origami.”
Morii and his team settled on the use of zinc-finger proteins as “adapters”. A polypeptide chain of zinc-finger protein grabs a zinc ion to form a stable compact fold; this fold referred to as a “zinc finger” and can bind to specific DNA patterns. It is possible to make zinc fingers that recognize any DNA pattern desired.
The scientists produced rectangular origami structures with several defined cavities. At these locations, the origamis contain various DNA-recognition patterns for different zinc fingers. The researchers then made proteins that contain zinc-finger units at one end and a fluorescing protein or biotin molecule at the other end. Biotin binds specifically to the large protein streptavidin. Atomic force microscopic images show that the streptavidin molecules always bind specifically to the intended cavity in the origami rectangle.
“Our results demonstrate that zinc fingers are suitable site-selective adapters for targeting specific locations within DNA-origami structures,” says Morii. “Several different adapters carrying different proteins can independently bind at defined locations on this type of nanostructure.”
Angewandte Chemie International Edition, Permalink to the article: http://dx.doi.org/10.1002/anie.201108199
Takashi Morii | Angewandte Chemie
Novel mechanisms of action discovered for the skin cancer medication Imiquimod
21.10.2016 | Technische Universität München
Second research flight into zero gravity
21.10.2016 | Universität Zürich
Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.
"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...
In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.
A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...
By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.
"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...
COMPAMED has become the leading international marketplace for suppliers of medical manufacturing. The trade fair, which takes place every November and is co-located to MEDICA in Dusseldorf, has been steadily growing over the past years and shows that medical technology remains a rapidly growing market.
In 2016, the joint pavilion by the IVAM Microtechnology Network, the Product Market “High-tech for Medical Devices”, will be located in Hall 8a again and will...
'Ferroelectric' materials can switch between different states of electrical polarization in response to an external electric field. This flexibility means they show promise for many applications, for example in electronic devices and computer memory. Current ferroelectric materials are highly valued for their thermal and chemical stability and rapid electro-mechanical responses, but creating a material that is scalable down to the tiny sizes needed for technologies like silicon-based semiconductors (Si-based CMOS) has proven challenging.
Now, Hiroshi Funakubo and co-workers at the Tokyo Institute of Technology, in collaboration with researchers across Japan, have conducted experiments to...
14.10.2016 | Event News
14.10.2016 | Event News
12.10.2016 | Event News
21.10.2016 | Health and Medicine
21.10.2016 | Information Technology
21.10.2016 | Materials Sciences