Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Planned Coincidence

22.05.2012
Antibody-based search for new chemical reactions

Many discoveries are made by chance, but it is also possible to help it along: The chance of finding something interesting increases when the number of experiments rises. French researchers have now applied this principle to the search for new chemical reactions.

In the journal Angewandte Chemie, they have introduced a new concept based on antibodies and a “sandwich” immunoassay.

Is there any value in randomly mixing substances together like an alchemist to see what happens? When it is carried out systematically and on a large scale, this promising approach, known as high-throughput screening, has become an established technique used in the search for pharmaceutical agents and catalysts.

This concept is now being applied more broadly to the search for novel types of chemical reactions, particularly in the search for new, easier, faster, or more elegant synthetic pathways for natural products, specialty chemicals, and drugs.

French scientists led by Frédéric Taran (Institute of Biology and Technology, Saclay, iBiTec-S, Gif-sur-Yvette) have now developed a new immunoassay-based approach to searching for new coupling reactions that link two organic molecules together.

Reactants A and B are added to the wells of a microtiter plate. In some wells, various transition metals are added as possible reaction promotors. Reactant A carries a marker that is recognized and bound by antibody AK1; reactant B carries a marker for antibody AK2. If a coupling occurs, the product has both markers. After the reaction, the solutions are transferred to new plates that are coated with AK1. After a washing step, only molecules with a binding site for AK1 remain on the plate.

A solution of AK2 is next applied, followed by another washing step. Wherever AK2 binds, a product must be present that carries both markers – the result is a “sandwich” in which the product is the filling between two antibody “slices” of bread. Successful reactions are made visible by an enzyme that is bound to AK2 and causes the color to change to yellow. Wherever the color is clearly yellow, the reaction product is analyzed to determine if the reaction that formed it is of a known type or is previously unknown.

In order to prove that this concept works, the researchers examined 2260 reactions in parallel. The reactants they selected have both conventional and unconventional reactive groups. They were thus able to identify two new types of reaction promoted by copper: the reaction of thioureas to form isoureas and a cyclization reaction to form thiazole derivatives from alkynes and N-hydroxy thioureas.

About the Author
Dr Frédéric Taran is head of a chemistry laboratory at the Life Science Division of the CEA located in Saclay, near Paris. He has been working in the fields of labelling, catalysis and reaction discovery, notably by the use of high-throughput screening techniques, for over 10 years.
Author: Frédéric Taran, CEA, iBiTecS, Gif-sur-Yvette (France), http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/units/molecular-labelling-and-bio-organic-chemistry-scbm/14c-labeling-laboratory-lmc/14c-labelling-f.-taran
Title: Reaction Discovery by Using a Sandwich Immunoassay
Angewandte Chemie International Edition, Permalink to the article: http://dx.doi.org/10.1002/anie.201201451

Frédéric Taran | Angewandte Chemie
Further information:
http://pressroom.angewandte.org

More articles from Life Sciences:

nachricht Topologische Quantenchemie
21.07.2017 | Max-Planck-Institut für Chemische Physik fester Stoffe

nachricht Topological Quantum Chemistry
21.07.2017 | Max-Planck-Institut für Chemische Physik fester Stoffe

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Manipulating Electron Spins Without Loss of Information

Physicists have developed a new technique that uses electrical voltages to control the electron spin on a chip. The newly-developed method provides protection from spin decay, meaning that the contained information can be maintained and transmitted over comparatively large distances, as has been demonstrated by a team from the University of Basel’s Department of Physics and the Swiss Nanoscience Institute. The results have been published in Physical Review X.

For several years, researchers have been trying to use the spin of an electron to store and transmit information. The spin of each electron is always coupled...

Im Focus: The proton precisely weighted

What is the mass of a proton? Scientists from Germany and Japan successfully did an important step towards the most exact knowledge of this fundamental constant. By means of precision measurements on a single proton, they could improve the precision by a factor of three and also correct the existing value.

To determine the mass of a single proton still more accurate – a group of physicists led by Klaus Blaum and Sven Sturm of the Max Planck Institute for Nuclear...

Im Focus: On the way to a biological alternative

A bacterial enzyme enables reactions that open up alternatives to key industrial chemical processes

The research team of Prof. Dr. Oliver Einsle at the University of Freiburg's Institute of Biochemistry has long been exploring the functioning of nitrogenase....

Im Focus: The 1 trillion tonne iceberg

Larsen C Ice Shelf rift finally breaks through

A one trillion tonne iceberg - one of the biggest ever recorded -- has calved away from the Larsen C Ice Shelf in Antarctica, after a rift in the ice,...

Im Focus: Laser-cooled ions contribute to better understanding of friction

Physics supports biology: Researchers from PTB have developed a model system to investigate friction phenomena with atomic precision

Friction: what you want from car brakes, otherwise rather a nuisance. In any case, it is useful to know as precisely as possible how friction phenomena arise –...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Closing the Sustainability Circle: Protection of Food with Biobased Materials

21.07.2017 | Event News

»We are bringing Additive Manufacturing to SMEs«

19.07.2017 | Event News

The technology with a feel for feelings

12.07.2017 | Event News

 
Latest News

NASA looks to solar eclipse to help understand Earth's energy system

21.07.2017 | Earth Sciences

Stanford researchers develop a new type of soft, growing robot

21.07.2017 | Power and Electrical Engineering

Vortex photons from electrons in circular motion

21.07.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>