Many discoveries are made by chance, but it is also possible to help it along: The chance of finding something interesting increases when the number of experiments rises. French researchers have now applied this principle to the search for new chemical reactions.
In the journal Angewandte Chemie, they have introduced a new concept based on antibodies and a “sandwich” immunoassay.
Is there any value in randomly mixing substances together like an alchemist to see what happens? When it is carried out systematically and on a large scale, this promising approach, known as high-throughput screening, has become an established technique used in the search for pharmaceutical agents and catalysts.
This concept is now being applied more broadly to the search for novel types of chemical reactions, particularly in the search for new, easier, faster, or more elegant synthetic pathways for natural products, specialty chemicals, and drugs.
French scientists led by Frédéric Taran (Institute of Biology and Technology, Saclay, iBiTec-S, Gif-sur-Yvette) have now developed a new immunoassay-based approach to searching for new coupling reactions that link two organic molecules together.
Reactants A and B are added to the wells of a microtiter plate. In some wells, various transition metals are added as possible reaction promotors. Reactant A carries a marker that is recognized and bound by antibody AK1; reactant B carries a marker for antibody AK2. If a coupling occurs, the product has both markers. After the reaction, the solutions are transferred to new plates that are coated with AK1. After a washing step, only molecules with a binding site for AK1 remain on the plate.
A solution of AK2 is next applied, followed by another washing step. Wherever AK2 binds, a product must be present that carries both markers – the result is a “sandwich” in which the product is the filling between two antibody “slices” of bread. Successful reactions are made visible by an enzyme that is bound to AK2 and causes the color to change to yellow. Wherever the color is clearly yellow, the reaction product is analyzed to determine if the reaction that formed it is of a known type or is previously unknown.
In order to prove that this concept works, the researchers examined 2260 reactions in parallel. The reactants they selected have both conventional and unconventional reactive groups. They were thus able to identify two new types of reaction promoted by copper: the reaction of thioureas to form isoureas and a cyclization reaction to form thiazole derivatives from alkynes and N-hydroxy thioureas.About the Author
Frédéric Taran | Angewandte Chemie
Nanoparticle Exposure Can Awaken Dormant Viruses in the Lungs
16.01.2017 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt
Cholera bacteria infect more effectively with a simple twist of shape
13.01.2017 | Princeton University
Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.
As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...
At TU Wien, an alternative for resource intensive formwork for the construction of concrete domes was developed. It is now used in a test dome for the Austrian Federal Railways Infrastructure (ÖBB Infrastruktur).
Concrete shells are efficient structures, but not very resource efficient. The formwork for the construction of concrete domes alone requires a high amount of...
Many pathogens use certain sugar compounds from their host to help conceal themselves against the immune system. Scientists at the University of Bonn have now, in cooperation with researchers at the University of York in the United Kingdom, analyzed the dynamics of a bacterial molecule that is involved in this process. They demonstrate that the protein grabs onto the sugar molecule with a Pac Man-like chewing motion and holds it until it can be used. Their results could help design therapeutics that could make the protein poorer at grabbing and holding and hence compromise the pathogen in the host. The study has now been published in “Biophysical Journal”.
The cells of the mouth, nose and intestinal mucosa produce large quantities of a chemical called sialic acid. Many bacteria possess a special transport system...
UMD, NOAA collaboration demonstrates suitability of in-orbit datasets for weather satellite calibration
"Traffic and weather, together on the hour!" blasts your local radio station, while your smartphone knows the weather halfway across the world. A network of...
Fiber-reinforced plastics (FRP) are frequently used in the aeronautic and automobile industry. However, the repair of workpieces made of these composite materials is often less profitable than exchanging the part. In order to increase the lifetime of FRP parts and to make them more eco-efficient, the Laser Zentrum Hannover e.V. (LZH) and the Apodius GmbH want to combine a new measuring device for fiber layer orientation with an innovative laser-based repair process.
Defects in FRP pieces may be production or operation-related. Whether or not repair is cost-effective depends on the geometry of the defective area, the tools...
10.01.2017 | Event News
09.01.2017 | Event News
05.01.2017 | Event News
16.01.2017 | Power and Electrical Engineering
16.01.2017 | Information Technology
16.01.2017 | Power and Electrical Engineering