Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Planned Coincidence

22.05.2012
Antibody-based search for new chemical reactions

Many discoveries are made by chance, but it is also possible to help it along: The chance of finding something interesting increases when the number of experiments rises. French researchers have now applied this principle to the search for new chemical reactions.

In the journal Angewandte Chemie, they have introduced a new concept based on antibodies and a “sandwich” immunoassay.

Is there any value in randomly mixing substances together like an alchemist to see what happens? When it is carried out systematically and on a large scale, this promising approach, known as high-throughput screening, has become an established technique used in the search for pharmaceutical agents and catalysts.

This concept is now being applied more broadly to the search for novel types of chemical reactions, particularly in the search for new, easier, faster, or more elegant synthetic pathways for natural products, specialty chemicals, and drugs.

French scientists led by Frédéric Taran (Institute of Biology and Technology, Saclay, iBiTec-S, Gif-sur-Yvette) have now developed a new immunoassay-based approach to searching for new coupling reactions that link two organic molecules together.

Reactants A and B are added to the wells of a microtiter plate. In some wells, various transition metals are added as possible reaction promotors. Reactant A carries a marker that is recognized and bound by antibody AK1; reactant B carries a marker for antibody AK2. If a coupling occurs, the product has both markers. After the reaction, the solutions are transferred to new plates that are coated with AK1. After a washing step, only molecules with a binding site for AK1 remain on the plate.

A solution of AK2 is next applied, followed by another washing step. Wherever AK2 binds, a product must be present that carries both markers – the result is a “sandwich” in which the product is the filling between two antibody “slices” of bread. Successful reactions are made visible by an enzyme that is bound to AK2 and causes the color to change to yellow. Wherever the color is clearly yellow, the reaction product is analyzed to determine if the reaction that formed it is of a known type or is previously unknown.

In order to prove that this concept works, the researchers examined 2260 reactions in parallel. The reactants they selected have both conventional and unconventional reactive groups. They were thus able to identify two new types of reaction promoted by copper: the reaction of thioureas to form isoureas and a cyclization reaction to form thiazole derivatives from alkynes and N-hydroxy thioureas.

About the Author
Dr Frédéric Taran is head of a chemistry laboratory at the Life Science Division of the CEA located in Saclay, near Paris. He has been working in the fields of labelling, catalysis and reaction discovery, notably by the use of high-throughput screening techniques, for over 10 years.
Author: Frédéric Taran, CEA, iBiTecS, Gif-sur-Yvette (France), http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/units/molecular-labelling-and-bio-organic-chemistry-scbm/14c-labeling-laboratory-lmc/14c-labelling-f.-taran
Title: Reaction Discovery by Using a Sandwich Immunoassay
Angewandte Chemie International Edition, Permalink to the article: http://dx.doi.org/10.1002/anie.201201451

Frédéric Taran | Angewandte Chemie
Further information:
http://pressroom.angewandte.org

More articles from Life Sciences:

nachricht Water forms 'spine of hydration' around DNA, group finds
26.05.2017 | Cornell University

nachricht How herpesviruses win the footrace against the immune system
26.05.2017 | Helmholtz-Zentrum für Infektionsforschung

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Can the immune system be boosted against Staphylococcus aureus by delivery of messenger RNA?

Staphylococcus aureus is a feared pathogen (MRSA, multi-resistant S. aureus) due to frequent resistances against many antibiotics, especially in hospital infections. Researchers at the Paul-Ehrlich-Institut have identified immunological processes that prevent a successful immune response directed against the pathogenic agent. The delivery of bacterial proteins with RNA adjuvant or messenger RNA (mRNA) into immune cells allows the re-direction of the immune response towards an active defense against S. aureus. This could be of significant importance for the development of an effective vaccine. PLOS Pathogens has published these research results online on 25 May 2017.

Staphylococcus aureus (S. aureus) is a bacterium that colonizes by far more than half of the skin and the mucosa of adults, usually without causing infections....

Im Focus: A quantum walk of photons

Physicists from the University of Würzburg are capable of generating identical looking single light particles at the push of a button. Two new studies now demonstrate the potential this method holds.

The quantum computer has fuelled the imagination of scientists for decades: It is based on fundamentally different phenomena than a conventional computer....

Im Focus: Turmoil in sluggish electrons’ existence

An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.

We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...

Im Focus: Wafer-thin Magnetic Materials Developed for Future Quantum Technologies

Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.

Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...

Im Focus: World's thinnest hologram paves path to new 3-D world

Nano-hologram paves way for integration of 3-D holography into everyday electronics

An Australian-Chinese research team has created the world's thinnest hologram, paving the way towards the integration of 3D holography into everyday...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Marine Conservation: IASS Contributes to UN Ocean Conference in New York on 5-9 June

24.05.2017 | Event News

AWK Aachen Machine Tool Colloquium 2017: Internet of Production for Agile Enterprises

23.05.2017 | Event News

Dortmund MST Conference presents Individualized Healthcare Solutions with micro and nanotechnology

22.05.2017 | Event News

 
Latest News

How herpesviruses win the footrace against the immune system

26.05.2017 | Life Sciences

Water forms 'spine of hydration' around DNA, group finds

26.05.2017 | Life Sciences

First Juno science results supported by University of Leicester's Jupiter 'forecast'

26.05.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>