New Pheromone Helps Female Flies Tell Suitors to ‘Buzz Off’

RELEVANCE:
The anti-aphrodisiac effects of copulation have been observed to last for over a week in female Drosophila melanogaster, even though the only previously identified fruit fly pheromone, cVA, stays active on the female for only 24 hours. The identification of the new pheromone, CH503, and the discovery that it remains active on the female for up to 10 days, may help solve this behavioral mystery.
PRINCIPAL INVESTIGATOR
Edward A. Kravitz, George Packer Berry Professor of Neurobiology, Harvard Medical School, http://www.hms.harvard.edu/bss/neuro/kravitz/
JOURNAL
Current Biology
FOR A COPY OF THE PAPER
veronica_meade-kelly@hms.harvard.edu
There she is again: the cute girl at the mall. Big eyes. Long legs. She smiles at you. You’re about to make your move… but wait! What’s she wearing? It’s a letterman jacket, one clearly belonging to a hulking football player named “Steve.” This girl is taken. Wisely, you move on.

Countless teen movies have told the same tale, but behind the fiction is an essential, biological reality: Humans base their behavioral decisions, such as whom to court, on cues gleaned from their environment. The same holds true for all of the animal world, as a paper due to be published this week in Current Biology reminds us. In it, Harvard Medical School (HMS) researchers, along with German colleagues, report on a newly discovered pheromone produced by male fruit flies. They found that the pheromone, which they named CH503 for its molecular mass, acts as the chemical equivalent of the “letterman jacket” when transferred to females during the mating process. CH503 remains on the female’s outer body, warding off male suitors for at least a week. This anti-aphrodisiac effect helps to account for previously noted mating behaviors in fruit flies that have until now gone unexplained.

Researchers discovered this unexpectedly while using a new form of high-resolution laser mass spectrometry to scan distinct regions on the fruit flies’ cuticle, or surface. Joanne Yew, at the time a postdoc in the lab of HMS Neurobiology professor Edward Kravitz, teamed with Klaus Dreisewerd and colleagues at the University of Münster for the study. They used the refined instrumentation, which allowed them to focus an ultraviolet, high-intensity laser on an area as small as 200 micrometers in diameter, to analyze and compare the chemical make-up of each discrete region. The new technology allowed the team to view the flies at high spatial resolution for the first time, and led to the discovery of nearly 30 new compounds not previously detected by traditional methods.

According to Yew, the technology also revealed a difference in the pheromone profiles of the leg and genital regions of the fruit flies. Among the compounds they found almost exclusively in the male genital region was cis-vaccenyl acetate (cVA), which has long been known to work as an anti-aphrodisiac in fruit flies when transferred to females during mating. A second compound — the hydrocarbon they came to identify as CH503 — was also discovered in the male genital region. Mass spectrometry revealed that this newly discovered compound was passed on to females during copulation, and remained on the surface of their bodies for at least 10 days after successful mating.

This led the researchers to hypothesize that the new compound might be the missing piece to a longstanding behavioral puzzle: The anti-aphrodisiac effects of copulation have been observed to last for over a week in fruit flies, even though cVA only stays active on the female for 24 hours. To test their theory, Yew’s team “perfumed virgin females with [CH503] and found that having this compound on the female’s surface inhibited courtship in males.”

Dr. Kravitz said of the findings, “Everyone already knew from behavioral experiments that the anti-aphrodisiac effects on female fruit flies can last up to a week, so it may be that this compound, CH503, explains why you have anti-aphrodisiac effects that last much longer. It also makes clear that cVA is only part of the story.”

The researchers hypothesize that their method might one day be used to identify pheromones from health-related insects such as mosquitoes, with possible implications for population control.

The paper is due to be published online July 16 in Current Biology.

This research is funded by a Human Frontier Science Program short-term fellowship; a National Institute of Mental Health National Research Service Award; and research grants from the National Institute of General Sciences, the National Science Foundation, and the Deutsche Forschungsgemeinschaft.

Written by Veronica Meade-Kelly

FULL CITATION

Current Biology, online July 16, scheduled to appear in the Aug. 11 print edition

“A new male sex-pheromone and novel cuticular cues for chemical communication in Drosophila”

Joanne Y. Yew (1*), Klaus Dreisewerd (2*), Heinrich Luftmann (3), Johannes Muething (4), Gottfried Pohlentz (2), and Edward A. Kravitz (1)

(1) Department of Neurobiology, Harvard Medical School, Boston, MA
(2) Institute of Medical Physics and Biophysics, University of Muenster, Muenster, Germany
(3) Institute of Organic Chemicstry, University of Muenster, Muenster, Germany
(4) Institute of Hygiene, University of Muenster, Muenster, Germany
*These authors contributed equally to this work
Harvard Medical School http://hms.harvard.edu has more than 7,500 full-time faculty working in 11 academic departments located at the School's Boston campus or in one of 47 hospital-based clinical departments at 17 Harvard-affiliated teaching hospitals and research institutes. Those affiliates include Beth Israel Deaconess Medical Center, Brigham and Women's Hospital, Cambridge Health Alliance, Children's Hospital Boston, Dana-Farber Cancer Institute, Forsyth Institute, Harvard Pilgrim Health Care, Hebrew SeniorLife, Joslin Diabetes Center, Judge Baker Children's Center, Massachusetts Eye and Ear Infirmary, Massachusetts General Hospital, McLean Hospital, Mount Auburn Hospital, Schepens Eye Research Institute, Spaulding Rehabilitation Hospital, and VA Boston Healthcare System.