Because olfactory receptors belong to a larger class of proteins that are involved in passing signals through the cell membrane, these devices could have applications beyond odor sensing, such as pharmaceutical research.
The research was led by professor A. T. Charlie Johnson, postdoctoral fellow Brett R. Goldsmith and graduate student Mitchell T. Lerner of the Department of Physics and Astronomy in the School of Arts and Sciences, along with assistant professor Bohdana M. Discher and postdoctoral fellow Joseph J. Mitala Jr. of the Department of Biophysics and Biochemistry at Penn’s Perelman School of Medicine. They collaborated with researchers from the Monell Chemical Senses Center, the University of Miami, the University of Illinois, Princeton University and two private companies, Nanosense Inc. and Evolved Machines Inc.
Their work was published in the journal ACS Nano.
The Penn team worked with olfactory receptors derived from mice, but all olfactory receptors are part of a class of proteins known as G Protein Coupled Receptors, or GPCRs. These receptors sit on the outer membrane of cells, where certain chemicals in the environment can bind to them. The binding action is the first step in a chemical cascade that leads to a cellular response; in the case of an olfactory receptor, this cascade leads to the perception of a smell.
The Penn team succeeded in building an interface between this complicated protein and a carbon nanotube transistor, allowing them to convert the chemical signals the receptor normally produces to electrical signals, which could be incorporated in any number of tools and gadgets.
“Our nanotech devices are read-out elements; they eavesdrop on what the olfactory receptors are doing, specifically what molecules are bound to them,” Johnson said.
As the particular GPCR the team worked with was an olfactory receptor, the test case for their nanotube device was to function as sensor for airborne chemicals.
“If there’s something in the atmosphere that wants to bind to this molecule, the signal we get through the nanotube is about what fraction of the time is something bound or not. That means we can get a contiguous read out that’s indicative of the concentration of the molecule in the air,” Johnson said.
While one could imagine scaling up these nanotube devices into a synthetic nose — making one for each of the approximately 350 olfactory GPCRs in a human nose, or the 1,000 found in a dog’s — Johnson thinks that medical applications are much closer to being realized.
“GPCRs are common drug targets,” he said. “Since they are known to be very important in cell-environment interactions, they’re very important in respect to disease pathology. In that respect, we now have a pathway into interrogating what those GPCRs actually respond to. You can imagine building a chip with many of these devices, each with different GPCRs, and exposing them all at once to various drugs to see which is effective at triggering a response.”
Figuring out what kinds of drugs bind most effectively to GPCRs is important because pathogens often attack through those receptors as well. The better a harmless chemical attaches to a relevant GPCR, the better it is at blocking the disease.
The Penn team also made a technical advancement in stabilizing GPCRs for future research.
“In the past, if you take a protein out of a cell and put it onto a device, it might last for a day. But here, we embedded it in a nanoscale artificial cell membrane, which is called a nanodisc,” Johnson said. “When we did that, they lasted for two and half months, instead of a day.”
Increasing the lifespans of such devices could be beneficial to two scientific fields with increasing overlap, as the as evidenced by the large, interdisciplinary research team involved in the study.
“The big picture is integrating nanotechnology with biology, “ Johnson said. “These complicated molecular machines are the prime method of communication between the interior of the cell and the exterior, and now we’re incorporating their functionality with our nanotech devices.”
In addition to Johnson, Goldsmith, Lerner, Discher and Mitala, the research was conducted by Jesusa Josue and Joseph G. Brand of Monell; Alan Gelperin of Monell and Princeton; Ana Castro and Charles W. Luetje of the University of Miami; Timothy H. Bayburt and Stephen G. Sligar of the University of Illinois, Urbana; Samuel M. Khamis of Adamant Technologies, Ryan A. Jones of Nanosense Inc.; and Paul A. Rhodes of Nanosense Inc. and Evolved Machines Inc.
The research was supported by the Defense Advanced Research Projects Agency’s RealNose project, Penn’s Nano/Bio Interface Center, the National Science Foundation and the Department of Defense.
Evan Lerner | EurekAlert!
Cloud Formation: How Feldspar Acts as Ice Nucleus
09.12.2016 | Karlsruher Institut für Technologie
Closing the carbon loop
08.12.2016 | University of Pittsburgh
In recent years, lasers with ultrashort pulses (USP) down to the femtosecond range have become established on an industrial scale. They could advance some applications with the much-lauded “cold ablation” – if that meant they would then achieve more throughput. A new generation of process engineering that will address this issue in particular will be discussed at the “4th UKP Workshop – Ultrafast Laser Technology” in April 2017.
Even back in the 1990s, scientists were comparing materials processing with nanosecond, picosecond and femtosesecond pulses. The result was surprising:...
Have you ever wondered how you see the world? Vision is about photons of light, which are packets of energy, interacting with the atoms or molecules in what...
A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.
Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...
In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.
“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...
The Max Planck Institute for Physics (MPP) is opening up a new research field. A workshop from November 21 - 22, 2016 will mark the start of activities for an innovative axion experiment. Axions are still only purely hypothetical particles. Their detection could solve two fundamental problems in particle physics: What dark matter consists of and why it has not yet been possible to directly observe a CP violation for the strong interaction.
The “MADMAX” project is the MPP’s commitment to axion research. Axions are so far only a theoretical prediction and are difficult to detect: on the one hand,...
16.11.2016 | Event News
01.11.2016 | Event News
14.10.2016 | Event News
08.12.2016 | Life Sciences
08.12.2016 | Physics and Astronomy
08.12.2016 | Materials Sciences