In a report in the current online issue of the journal Nature Methods, Dr. Hugo Bellen (http://flypush.imgen.bcm.tmc.edu/lab/), a professor of molecular and human genetics at BCM and a Howard Hughes Medical Institute investigator, and his colleagues describe the new libraries.
P[acman]– developed by Dr. Koen Venken (http://flypush.imgen.bcm.tmc.edu/lab/koenv/index.html) in Bellen's laboratory– allows scientists to study large chunks of DNA in living flies. The vector – officially P/phiC31 artificial chromosome for manipulation – combines different technologies: a specially designed bacterial artificial chromosome (BAC) that allows maintenance of large pieces of DNA in bacteria, recombineering that allows the manipulation of large pieces of DNA in bacteria, and the ability to insert the genomic DNA into the genome of the fly at a specific site using phiC31-mediated transgenesis.
Venken adapted the P[acman] vector to create genomic libraries, so that a researcher can choose a gene and find the corresponding clones in the library that cover that gene. Their collaborators at Lawrence Berkeley National Laboratory, Drs. Roger Hoskins and Joseph Carlson, played a key role in the design, construction, and annotation of the libraries.
"You can insert a single copy of a gene and rescue a mutation, or do a structure/function analysis of the gene," Bellen said. "If you don't know where the gene is expressed, you can tag it, put it back and locate where it is expressed."
The library is available at http://pacmanfly.org/.
Others who took part in this work include Karen L. Schulze, Hongling Pan and Yuchun He of BCM, Ken Wan (LBNL), Rebecca Spokony and Kevin P. White of the University of Chicago, and Maxim Koriabine and Pieter J. de Jong of Children's Hospital Oakland Research Institute in California.
Funding for this work came from the Howard Hughes Medical Institute, the National Institutes of Health and the BCM Intellectual and Developmental Disabilities Research Center.
When the embargo lifts, the report will be available at http://www.nature.com/nmeth/index.html
For more information on basic science research at Baylor College of Medicine, please go to www.bcm.edu/fromthelab.
First time-lapse footage of cell activity during limb regeneration
25.10.2016 | eLife
Phenotype at the push of a button
25.10.2016 | Institut für Pflanzenbiochemie
Ultrafast lasers have introduced new possibilities in engraving ultrafine structures, and scientists are now also investigating how to use them to etch microstructures into thin glass. There are possible applications in analytics (lab on a chip) and especially in electronics and the consumer sector, where great interest has been shown.
This new method was born of a surprising phenomenon: irradiating glass in a particular way with an ultrafast laser has the effect of making the glass up to a...
Terahertz excitation of selected crystal vibrations leads to an effective magnetic field that drives coherent spin motion
Controlling functional properties by light is one of the grand goals in modern condensed matter physics and materials science. A new study now demonstrates how...
Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.
"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...
In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.
A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...
By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.
"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...
14.10.2016 | Event News
14.10.2016 | Event News
12.10.2016 | Event News
25.10.2016 | Earth Sciences
25.10.2016 | Power and Electrical Engineering
25.10.2016 | Process Engineering