This powerful tool, described in a new article from Nature Methods, promises to help neuroscientists identify and monitor the behavior of neural circuits involved in motor activity, sensory perception and other functions.
Researchers have historically analyzed the complex electrical activity of the brain using an invasive approach involving tiny electrodes, whose large size relative to individual nerve cells has limited the number of locations from which neuronal activity can be sampled. Optimal imaging methods overcome this limitation with molecular size probes that transform the electrical signals into an optical reporter signal.
The voltage-sensitive fluorescent proteins (VSFPs) developed by Thomas Knöpfel’s team at the RIKEN Brain Science Institute represent an important step in this direction. These are engineered proteins that reside within the membranes of neurons, each of which is fused to two different fluorescent proteins. Whenever a neuron receives a stimulatory signal, the resulting change in voltage potential in the cell membrane causes the VSFPs to rearrange into a configuration that causes a readily detectable change in the optical signal generated by the VSFP, in a phenomenon known as Förster Resonance Energy Transfer.
Knöpfel’s laboratory pionered the development of these sensors for more than 10 years but up to now the function of these probes was only demonstrated by recording electrical activity from 2-dimensional networks of cultured nerve cells. In the latest edition of Nature Methods, the team presents the first experimental confirmation that these probes are able to report electrical activity of nerve cells in the brains of living mice. The researchers used genetically modified mice to localize the VSFP probe within specific subsets of cortical neurons within a brain area called the somatosensory cortex. Each mouse whisker is wired to discrete neural circuits in the somatosensory cortex, and the researchers found that they could readily detect changes in the membrane voltage of these circuit elements as each whisker was manipulated. Based on these experiments, they were essentially able to reconstruct maps of the cell populations that operate as ‘receptive areas’ for individual whiskers.
Being genetically encoded, VSFPs offer several advantages over other commonly-used approaches to monitoring neuronal activity. They can essentially be ‘programmed’ for selective expression within specific subtypes of neurons or particular regions of the brain, and could be used to chart long-range neural circuits extending over considerable distances, unlike fluorescent dyes that label cells non-specifically and can only be applied within a relatively limited volume of the brain. Other genetically-encoded sensors have been developed that respond to calcium flux in the immediate aftermath of neuronal firing, but these represent indirect indicators and generally respond more slowly to neuronal activity.
Given the high degree of spatial and temporal resolution displayed by the VSFPs in this study, Knöpfel is confident that they will prove a useful tool for researchers hoping to understand how patterns of neuronal activity correlate with behavior or physiological changes in the living brain. “The ability of VSFPs to report faster signals, along with genetic targeting, will allow new approaches to the study of the dynamic interaction of assemblies of neurons,” he says. “This will facilitate the investigation of fundamental questions of information processing in the brain, such as the circuit operations involved in sensing our environment and generation of body movements, but will also be applicable to directly visualize cognitive functions.”
For more information, please contact:Dr. Thomas Knöpfel
In living color: Brightly-colored bacteria could be used to 'grow' paints and coatings
20.02.2018 | University of Cambridge
Computers aid discovery of new, inexpensive material to make LEDs with high color quality
20.02.2018 | University of California - San Diego
For the first time, a team of researchers at the Max-Planck Institute (MPI) for Polymer Research in Mainz, Germany, has succeeded in making an integrated circuit (IC) from just a monolayer of a semiconducting polymer via a bottom-up, self-assembly approach.
In the self-assembly process, the semiconducting polymer arranges itself into an ordered monolayer in a transistor. The transistors are binary switches used...
Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale
Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...
For photographers and scientists, lenses are lifesavers. They reflect and refract light, making possible the imaging systems that drive discovery through the microscope and preserve history through cameras.
But today's glass-based lenses are bulky and resist miniaturization. Next-generation technologies, such as ultrathin cameras or tiny microscopes, require...
Scientists from the University of Zurich have succeeded for the first time in tracking individual stem cells and their neuronal progeny over months within the intact adult brain. This study sheds light on how new neurons are produced throughout life.
The generation of new nerve cells was once thought to taper off at the end of embryonic development. However, recent research has shown that the adult brain...
Theoretical physicists propose to use negative interference to control heat flow in quantum devices. Study published in Physical Review Letters
Quantum computer parts are sensitive and need to be cooled to very low temperatures. Their tiny size makes them particularly susceptible to a temperature...
15.02.2018 | Event News
13.02.2018 | Event News
12.02.2018 | Event News
20.02.2018 | Life Sciences
20.02.2018 | Life Sciences
20.02.2018 | Physics and Astronomy