Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New molecular mechanism for the regulation of plastic phases in brain development discovered.

16.06.2015

Scientists from Göttingen University discovered a new mechanism to control time windows of high adaptability in the brain: a signaling scaffold of the postsynapse governs the duration of critical periods in early brain development. Published in the renowned American journal Proceedings of the National Academy of Sciences USA (PNAS).

Our ability to see or hear only develops if also the brain has learned to “see” or “hear”. To succeed, this learning has to happen in particular time windows of high adaptability (plasticity), so-called “critical periods”, during brain development.


Down-regulation of PSD-95 expression in the visual cortex of adult mice (PSD-95 knock-down, right), restores juvenile plasticity, i.e. adaptability, in their visual cortex.

Loewel/Schlüter

The mechanisms underlying the regulation of these critical periods and which factors govern their termination is not only important from a basic research perspective, but in addition, this knowledge may open up new avenues to develop clinically relevant concepts to promote regeneration and rehabilitation for diseased and injured brains.

The research groups of Prof. Dr. Siegrid Löwel, Department of Systems Neuroscience at the Institut für Zoologie und Anthropologie of Göttingen University, and Dr. Dr. Oliver Schlüter, Research Group Molecular Neurobiology at the European Neuroscience Institute Göttingen, have discovered a new mechanism controlling the timing of „critical periods” during brain development.

The researchers identified how the maturation of nerve cell contacts (synapses) and the termination of the critical period for a particular form of adaptability (plasticity) in the visual cortex are governed.

The prevailing notion is that early sensitive phases of high adaptability end because the inhibitory circuitry matures and so-called plasticity “brakes” are expressed. Results of the two research groups now provided direct evidence that a single molecule – the signaling scaffold postsynaptic density 95 (PSD-95) - is sufficient to govern the duration of a critical period, independently of cortical inhibition.

In comparative studies, mice without PSD-95, so-called PSD-95 knock-out mice, displayed a lifelong and juvenile plasticity in the visual cortex, similar to young wild type mice during their critical period. The findings were obtained in a close cooperation of the research groups of Prof. Dr. Siegrid Löwel and Dr. Dr. Oliver Schlüter, within the Collaborative Research Center „Cellular Mechanisms of Sensory Processing” (CRC 889). The research results were published in the renowned American journal PNAS.

Original publication:
Huang, X.*, Stodieck, S.K.*, Goetze, B., Schmidt, K.-F., Cui, L., Wenzel, C., Hosang, L., Dong, Y., Löwel, S.* and Schlüter, O.M.* (2015) The Progressive Mat-uration of Silent Synapses Governs the Duration of a Critical Period. Proc. Natl. Acad. Sci. U.S.A. 2015 May 26. pii: 201506488. [Epub ahead of print]. *equal contribution.

WHAT IS THE FUNCTION OF PSD-95?
The protein PSD-95 is necessary for the maturation of contact sites between nerve cell (synapses). „Without PSD-95 half of the visual cortical synapses remain „silent” lifelong. Such „silent synapses“ contain only NMDA-receptors, which are not activated under basal conditions. Hence, the silent synapses do not trigger electrical discharges at the target cell – and thus do not transmit any information“, explains Dr. Dr. Oliver Schlüter. „Typically, synaptic maturation includes the integration of AMPA-receptors into the postsynapse. PSD-95 is essential for this step.“ The „matured“ synapses are no longer silent, but transmit signals from one nerve cell to the next.

“We were also able to show that PSD-95 is not only essential for the maturation of young synapses but also for the stabilization of matured synapses: Down-regulation of PSD-95 expression (knock-down) in already matured nerve cell circuits, that is after the critical period, restored both the “young” synaptic state and juvenile plasticity”, explains Prof. Dr. Siegrid Löwel.

CRC 889: COMBINING EXPERTISE AS THE BASIS OF SUCCESS
Key to arriving at these new insights about „critical periods“ during brain development was the combination of many different techniques within the CRC 889: used methods range from the manipulation of the expression of single molecules to patch-clamp recordings in brain slices and biochemical analyses to optical imaging of neuronal activity in the cortex and behavioral measurements of visual capabilities of the genetically modified mice. Only the combined expertise and intensive collaboration of the two Göttingen laboratories of Prof. Dr. Siegrid Löwel and Dr. Dr. Oliver Schlüter made it possible to perform all the necessary experiments for such a comprehensive analysis. „This close teamwork is a prime example of what a CRC can optimally achieve”, says Prof. Dr. Tobias Moser, speaker of the CRC 889. „By bringing together research groups with different expertise who work together on a new subject, you can achieve more than any single group would have been able to do on its own.“

BACKGROUND INFORMATION
Nerve cell networks in the mammalian cortex are initially generated primarily under genetic control, and then need experience and training to form their functional and structural architecture and to optimize their capabilities. This experience-dependent refinement is considered to be a general developmental process for all functional cortical domains and typically peaks during their respective time window, so-called „critical periods”: during these phases, young brains are significantly more „plastic“, i.e. more adaptive, than older brains. Known examples for such critical periods span functional domains as diverse as filial imprinting and courtship song learning in birds; cognitive functions, such as linguistic or musical skills in humans; and likely best studied, the different features of sensory modalities. Critical periods are characterized by the absolute requirement for experience in a restricted time window for neural network and performance optimization. Lack of specific sensory stimuli during these time windows, e.g. of visual experience (caused by a cloudy lens or a drooping eyelid) during development of the visual cortex, i.e. the region analyzing visual stimuli, can cause irreversible visual impairment during later life.

CRC 889 „CELLULAR MECHANISMS OF SENSORY PROCESSING“
The interdisciplinary Collaborative Research Center „Cellular Mechanisms of Sensory Processing“ (CRC 889) aims to better understand the major human senses „vision, hearing, smell, touch“. Since 2011, research of the CRC 889 is supported by the German Research Foundation (DFG). Prof. Dr. Tobias Moser, director of the Institute for Auditory Neurosciences of the University Medical Center Göttingen (UMG) is the Speaker of the CRC. Scientists from 23 different neuroscience research groups located in Göttingen cooperate in 21 projects. Researchers from hospitals and Institutes of the Medical School, from the European Neuroscience Institute (ENI-G), the Biological Faculty of Göttingen University, from the Max-Planck-Institut für biophysikalische Chemie, the Max-Planck-Institut für experimentelle Medizin as well as from the Max-Planck-Institut für Dynamik und Selbstorganisation and the German Primate Center participate.

FIGURE: Down-regulation of PSD-95 expression in the visual cortex of adult mice (PSD-95 knock-down, right), restores juvenile plasticity, i.e. adaptability, in their visual cortex. This neuronal plasticity can be visualized using modern imaging techniques: a specific visual stimulation of one eye (ipsi, white dot) activates the visual cortex equally well (dark grey) as the formerly stronger eye (contra, black dot); moreover, map colors change from warm (yellow-orange) to cold (blue-green) colors. In mice with normal PSD-95 expression (wild type, left), visual cortical activation is not changed. Figure modified from Huang et al. (2015) PNAS, published ahead of print May 26, 2015, doi:10.1073/pnas.1506488112.

FURTHER INFORMATIONEN:
Prof. Dr. Siegrid Löwel
Georg-August-Universität Göttingen
Fakultät für Biologie und Psychologie
Johann-Friedrich-Blumenbach-Institut für Zoologie und Anthropologie
Department Systems Neuroscience
Von-Siebold-Straße 6, 37075 Göttingen, phone +49 (0) 551/39-20161/-20160
sloewel@gwdg.de, Internet: http://systemsneuroscience.uni-goettingen.de/

Dr. Dr. Oliver M. Schlüter
European Neuroscience Institute (ENI)
Molecular Neurobiology
Grisebachstr. 5, 37077 Göttingen, phone +49 (0) 551/39-10374
oschlue@gwdg.de, Internet: http://www.eni.gwdg.de/index.php?id=101

Stefan Weller | idw - Informationsdienst Wissenschaft

More articles from Life Sciences:

nachricht Modern genetic sequencing tools give clearer picture of how corals are related
17.08.2017 | University of Washington

nachricht The irresistible fragrance of dying vinegar flies
16.08.2017 | Max-Planck-Institut für chemische Ökologie

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Fizzy soda water could be key to clean manufacture of flat wonder material: Graphene

Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.

As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...

Im Focus: Exotic quantum states made from light: Physicists create optical “wells” for a super-photon

Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.

Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...

Im Focus: Circular RNA linked to brain function

For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.

While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...

Im Focus: RAVAN CubeSat measures Earth's outgoing energy

An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.

The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...

Im Focus: Scientists shine new light on the “other high temperature superconductor”

A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.

Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Call for Papers – ICNFT 2018, 5th International Conference on New Forming Technology

16.08.2017 | Event News

Sustainability is the business model of tomorrow

04.08.2017 | Event News

Clash of Realities 2017: Registration now open. International Conference at TH Köln

26.07.2017 | Event News

 
Latest News

Gold shines through properties of nano biosensors

17.08.2017 | Physics and Astronomy

Greenland ice flow likely to speed up: New data assert glaciers move over sediment, which gets more slippery as it gets wetter

17.08.2017 | Earth Sciences

Mars 2020 mission to use smart methods to seek signs of past life

17.08.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>