Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New microscopy system captures 'lost' fluorescence, improving resolution

12.08.2016

Taking a cue from medical imaging, scientists have invented a multi-view microscope that captures higher-resolution, 3D images of live cells and tissues without upping the dose of potentially harmful radiation the specimens receive.

The researchers, who work collaboratively at the Marine Biological Laboratory's Whitman Center, published their results this week in the journal Optica.


This is a macrophage actin labeled with green fluorescent protein, imaged with the new triSPIM microscope (left) and the diSPIM (right).

Credit: Yicong Wu and Valentin Jaumouille

"Everybody knows fluorescence imaging is inefficient in that the microscope only captures a portion of the light (spewing off the specimen)," says senior author Hari Shroff of the National Institute of Biomedical Imaging and Bioengineering. "In this paper, we showed you can not only capture that lost light, but use computation to fuse it to the existing image and make the image sharper."

Developed by Yicong Wu, a staff scientist in Shroff's lab, the new system achieved resolution of up to 235 x 235 x 340 nanometers, which is double the volumetric resolution of traditional fluorescence microscopy methods.

To collect more of the available light (which, in turn, provides more information about the specimen), the new microscope has three objective lenses acquiring views of the sample simultaneously. The views are then aligned and merged by a computational process known as deconvolution.

Those computations were worked out in collaboration with co-author Patrick La Rivière of the University of Chicago's Radiology Department, who typically develops algorithms for improving "dose efficiency" in human-scale medical imaging, such as CAT scans.

"In medical imaging, we are always worried about dose, about capturing every X-ray [used on the patient to improve scan resolution]. We are concerned with 'How can we do more with less?'" La Rivière says.

In microscopy, the amount of light used presents similar concerns. "If you use very intense illuminations to image something microscopic like a worm embryo, you might change its biology or even kill it. You need to be dose efficient with your light," La Rivière says.

La Rivière and Shroff began collaborating at the MBL in 2014, initially on algorithms to improve Shroff's diSPIM microscope (which has two objective lenses) and eventually on the new three-lensed microscope (called triSPIM).

La Rivière this year was named an MBL Fellow. Shroff is an MBL Whitman Center Scientist and co-director of the MBL's Optical Microscopy and Imaging in the Biomedical Sciences course.

###

Citation:

Yicong Wu, P. Chandris, P.W. Winter, E.Y. Kim, V. Jaumouillé, A. Kumar, M. Guo, J.M. Leung, C. Smith, I. Rey-Suarez, H. Liu, C.M. Waterman, K.S. Ramamurthi, P. La Riviere, H. Shroff (2016) Simultaneous multi-view capture and fusion improves spatial resolution in wide-field and light-sheet microscopy. Optica 3, 8: 897-920; doi: 10.1364/OPTICA.3.000897

The Marine Biological Laboratory (MBL) is dedicated to scientific discovery - exploring fundamental biology, understanding marine biodiversity and the environment, and informing the human condition through research and education. Founded in Woods Hole, Massachusetts in 1888, the MBL is a private, nonprofit institution and an affiliate of the University of Chicago.

Media Contact

Diana Kenney
dkenney@mbl.edu
508-289-7139

 @mblscience

http://www.mbl.edu 

Diana Kenney | EurekAlert!

More articles from Life Sciences:

nachricht Symbiotic bacteria: from hitchhiker to beetle bodyguard
28.04.2017 | Johannes Gutenberg-Universität Mainz

nachricht Nose2Brain – Better Therapy for Multiple Sclerosis
28.04.2017 | Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Making lightweight construction suitable for series production

More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.

Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...

Im Focus: Wonder material? Novel nanotube structure strengthens thin films for flexible electronics

Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.

"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...

Im Focus: Deep inside Galaxy M87

The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.

Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...

Im Focus: A Quantum Low Pass for Photons

Physicists in Garching observe novel quantum effect that limits the number of emitted photons.

The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...

Im Focus: Microprocessors based on a layer of just three atoms

Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.

Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Fighting drug resistant tuberculosis – InfectoGnostics meets MYCO-NET² partners in Peru

28.04.2017 | Event News

Expert meeting “Health Business Connect” will connect international medical technology companies

20.04.2017 | Event News

Wenn der Computer das Gehirn austrickst

18.04.2017 | Event News

 
Latest News

Wireless power can drive tiny electronic devices in the GI tract

28.04.2017 | Medical Engineering

Ice cave in Transylvania yields window into region's past

28.04.2017 | Earth Sciences

Nose2Brain – Better Therapy for Multiple Sclerosis

28.04.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>