Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New microscopy system captures 'lost' fluorescence, improving resolution

12.08.2016

Taking a cue from medical imaging, scientists have invented a multi-view microscope that captures higher-resolution, 3D images of live cells and tissues without upping the dose of potentially harmful radiation the specimens receive.

The researchers, who work collaboratively at the Marine Biological Laboratory's Whitman Center, published their results this week in the journal Optica.


This is a macrophage actin labeled with green fluorescent protein, imaged with the new triSPIM microscope (left) and the diSPIM (right).

Credit: Yicong Wu and Valentin Jaumouille

"Everybody knows fluorescence imaging is inefficient in that the microscope only captures a portion of the light (spewing off the specimen)," says senior author Hari Shroff of the National Institute of Biomedical Imaging and Bioengineering. "In this paper, we showed you can not only capture that lost light, but use computation to fuse it to the existing image and make the image sharper."

Developed by Yicong Wu, a staff scientist in Shroff's lab, the new system achieved resolution of up to 235 x 235 x 340 nanometers, which is double the volumetric resolution of traditional fluorescence microscopy methods.

To collect more of the available light (which, in turn, provides more information about the specimen), the new microscope has three objective lenses acquiring views of the sample simultaneously. The views are then aligned and merged by a computational process known as deconvolution.

Those computations were worked out in collaboration with co-author Patrick La Rivière of the University of Chicago's Radiology Department, who typically develops algorithms for improving "dose efficiency" in human-scale medical imaging, such as CAT scans.

"In medical imaging, we are always worried about dose, about capturing every X-ray [used on the patient to improve scan resolution]. We are concerned with 'How can we do more with less?'" La Rivière says.

In microscopy, the amount of light used presents similar concerns. "If you use very intense illuminations to image something microscopic like a worm embryo, you might change its biology or even kill it. You need to be dose efficient with your light," La Rivière says.

La Rivière and Shroff began collaborating at the MBL in 2014, initially on algorithms to improve Shroff's diSPIM microscope (which has two objective lenses) and eventually on the new three-lensed microscope (called triSPIM).

La Rivière this year was named an MBL Fellow. Shroff is an MBL Whitman Center Scientist and co-director of the MBL's Optical Microscopy and Imaging in the Biomedical Sciences course.

###

Citation:

Yicong Wu, P. Chandris, P.W. Winter, E.Y. Kim, V. Jaumouillé, A. Kumar, M. Guo, J.M. Leung, C. Smith, I. Rey-Suarez, H. Liu, C.M. Waterman, K.S. Ramamurthi, P. La Riviere, H. Shroff (2016) Simultaneous multi-view capture and fusion improves spatial resolution in wide-field and light-sheet microscopy. Optica 3, 8: 897-920; doi: 10.1364/OPTICA.3.000897

The Marine Biological Laboratory (MBL) is dedicated to scientific discovery - exploring fundamental biology, understanding marine biodiversity and the environment, and informing the human condition through research and education. Founded in Woods Hole, Massachusetts in 1888, the MBL is a private, nonprofit institution and an affiliate of the University of Chicago.

Media Contact

Diana Kenney
dkenney@mbl.edu
508-289-7139

 @mblscience

http://www.mbl.edu 

Diana Kenney | EurekAlert!

More articles from Life Sciences:

nachricht The irresistible fragrance of dying vinegar flies
16.08.2017 | Max-Planck-Institut für chemische Ökologie

nachricht How protein islands form
15.08.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Exotic quantum states made from light: Physicists create optical “wells” for a super-photon

Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.

Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...

Im Focus: Circular RNA linked to brain function

For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.

While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...

Im Focus: RAVAN CubeSat measures Earth's outgoing energy

An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.

The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...

Im Focus: Scientists shine new light on the “other high temperature superconductor”

A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.

Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...

Im Focus: Scientists improve forecast of increasing hazard on Ecuadorian volcano

Researchers from the University of Miami (UM) Rosenstiel School of Marine and Atmospheric Science, the Italian Space Agency (ASI), and the Instituto Geofisico--Escuela Politecnica Nacional (IGEPN) of Ecuador, showed an increasing volcanic danger on Cotopaxi in Ecuador using a powerful technique known as Interferometric Synthetic Aperture Radar (InSAR).

The Andes region in which Cotopaxi volcano is located is known to contain some of the world's most serious volcanic hazard. A mid- to large-size eruption has...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Call for Papers – ICNFT 2018, 5th International Conference on New Forming Technology

16.08.2017 | Event News

Sustainability is the business model of tomorrow

04.08.2017 | Event News

Clash of Realities 2017: Registration now open. International Conference at TH Köln

26.07.2017 | Event News

 
Latest News

New thruster design increases efficiency for future spaceflight

16.08.2017 | Physics and Astronomy

Transporting spin: A graphene and boron nitride heterostructure creates large spin signals

16.08.2017 | Materials Sciences

A new method for the 3-D printing of living tissues

16.08.2017 | Interdisciplinary Research

VideoLinks
B2B-VideoLinks
More VideoLinks >>>