Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Nanobodies from Camels Enable the Study of Organ Growth

10.11.2015

Researchers at the Biozentrum of the University of Basel have developed a new technique using nanobodies. Employing the so-called “Morphotrap”, the distribution of the morphogen Dpp, which plays an important role in wing development, could be selectively manipulated and analyzed for the first time in the fruit fly. In the future, this tool may be applied for many further investigations of organ growth. The results of the study have been published in the current issue of “Nature”.

The two basic processes that control organ development are the regulation of growth and of the spatial pattern. The research group of Prof. Markus Affolter at the Biozentrum, University of Basel, has now developed a method named “Morphotrap” to study wing development in the fruit fly.


Drosophila wing size control depends on the spreading of the Dpp morphogen.

University of Basel, Biozentrum

Their results demonstrate that the signaling molecule Dpp, a so-called morphogen, influences growth in the center of the wing imaginal disc but not in the peripheral regions. It is the first time that an anti-GFP nanobody has been successfully employed in such an investigation. This tool also holds promise for future studies on organ development.

The new method “Morphotrap”: Nanobodies to study growth

Nanobodies are small antibody fragments derived from camels. They enable the research team of Markus Affolter to manipulate molecules in the living organism. The so-called “Morphotrap” method employs anti-GFP nanobodies. Using these Nanobodies, the functions of GFP-tagged proteins in living organisms can be studied faster and more effectively than by conventional methods.

“These anti-GFP nanobodies inhibit the dispersal of the morphogen Dpp at different locations in the wing. Therefore they allow us to identify the influence of Dpp spreading on wing growth,” explains Stefan Harmansa, the first author of the study.

Morphogen Dpp regulates growth in the middle of the imaginal disc

To determine the influence of the morphogen Decapentaplegic (Dpp) in more detail, the Affolter group examined the wing disc of the fruit fly, called the imaginal disc. This is the precursor tissue of the wing of the adult fly and serves as a model for studies on organ development.

“Our findings demonstrate that the morphogen Dpp only affects growth in the center of the imaginal disc. Growth continues in the periphery even when we fully block Dpp dispersal into this regions,” explains Harmansa. “Now, by employing anti GFP nanobodies, we have been able to show to which extent the morphogen Dpp determines the wing size and consequently we could disprove one of the two predominant theories in this field,” says Harmansa.

The fact that anti GFP-nanobodies can successfully be applied for research in complex living organism is a great achievement. Affolter also plans to apply this technique in future research: “In a next step, we will investigate at what time in development Dpp acts to control central growth. The correlation between the spatial and temporal influence of Dpp will provide new insights into organ growth and may uncover possible causes of organ malformation,” says Affolter.

Original source
Stefan Harmansa, Fisun Hamaratoglu, Markus Affolter & Emmanuel Caussinus
Dpp spreading is required for medial but not for lateral wing disc growth
Nature (2015), doi: 10.1038/nature15712

Further information
Prof. Dr. Markus Affolter, University of Basel, Biozentrum, tel. +41 61 267 20 72, email: markus.affolter@unibas.ch
Heike Sacher, University of Basel, Biozentrum, tel. +41 61 267 14 49, email: heike.sacher@unibas.ch

Weitere Informationen:

https://www.unibas.ch/en/News-Events/News/Uni-Research/Tuberculosis-bacteria-out...

Reto Caluori | Universität Basel

More articles from Life Sciences:

nachricht How brains surrender to sleep
23.06.2017 | IMP - Forschungsinstitut für Molekulare Pathologie GmbH

nachricht A new technique isolates neuronal activity during memory consolidation
22.06.2017 | Spanish National Research Council (CSIC)

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Can we see monkeys from space? Emerging technologies to map biodiversity

An international team of scientists has proposed a new multi-disciplinary approach in which an array of new technologies will allow us to map biodiversity and the risks that wildlife is facing at the scale of whole landscapes. The findings are published in Nature Ecology and Evolution. This international research is led by the Kunming Institute of Zoology from China, University of East Anglia, University of Leicester and the Leibniz Institute for Zoo and Wildlife Research.

Using a combination of satellite and ground data, the team proposes that it is now possible to map biodiversity with an accuracy that has not been previously...

Im Focus: Climate satellite: Tracking methane with robust laser technology

Heatwaves in the Arctic, longer periods of vegetation in Europe, severe floods in West Africa – starting in 2021, scientists want to explore the emissions of the greenhouse gas methane with the German-French satellite MERLIN. This is made possible by a new robust laser system of the Fraunhofer Institute for Laser Technology ILT in Aachen, which achieves unprecedented measurement accuracy.

Methane is primarily the result of the decomposition of organic matter. The gas has a 25 times greater warming potential than carbon dioxide, but is not as...

Im Focus: How protons move through a fuel cell

Hydrogen is regarded as the energy source of the future: It is produced with solar power and can be used to generate heat and electricity in fuel cells. Empa researchers have now succeeded in decoding the movement of hydrogen ions in crystals – a key step towards more efficient energy conversion in the hydrogen industry of tomorrow.

As charge carriers, electrons and ions play the leading role in electrochemical energy storage devices and converters such as batteries and fuel cells. Proton...

Im Focus: A unique data centre for cosmological simulations

Scientists from the Excellence Cluster Universe at the Ludwig-Maximilians-Universität Munich have establised "Cosmowebportal", a unique data centre for cosmological simulations located at the Leibniz Supercomputing Centre (LRZ) of the Bavarian Academy of Sciences. The complete results of a series of large hydrodynamical cosmological simulations are available, with data volumes typically exceeding several hundred terabytes. Scientists worldwide can interactively explore these complex simulations via a web interface and directly access the results.

With current telescopes, scientists can observe our Universe’s galaxies and galaxy clusters and their distribution along an invisible cosmic web. From the...

Im Focus: Scientists develop molecular thermometer for contactless measurement using infrared light

Temperature measurements possible even on the smallest scale / Molecular ruby for use in material sciences, biology, and medicine

Chemists at Johannes Gutenberg University Mainz (JGU) in cooperation with researchers of the German Federal Institute for Materials Research and Testing (BAM)...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Plants are networkers

19.06.2017 | Event News

Digital Survival Training for Executives

13.06.2017 | Event News

Global Learning Council Summit 2017

13.06.2017 | Event News

 
Latest News

Quantum thermometer or optical refrigerator?

23.06.2017 | Physics and Astronomy

A 100-year-old physics problem has been solved at EPFL

23.06.2017 | Physics and Astronomy

Equipping form with function

23.06.2017 | Information Technology

VideoLinks
B2B-VideoLinks
More VideoLinks >>>