Zehr said the newly described organism seems to be an atypical member of the cyanobacteria, a group of photosynthetic bacteria formerly known as blue-green algae. Unlike all other known free-living cyanobacteria, this one lacks some of the genes needed to carry out photosynthesis, the process by which plants use light energy to make sugars out of carbon dioxide and water. The mysterious microbe can do something very important, though: It provides natural fertilizer to the oceans by "fixing" nitrogen from the atmosphere into a form useable by other organisms.
"For it to have such an unusual metabolism is very exciting," Zehr said. "We're trying to understand how something like this can live and grow with so many missing parts."
A paper describing the new findings appears in the November 14 issue of the journal Science.
Earlier research by Zehr's group had revealed surprisingly large numbers of novel nitrogen-fixing cyanobacteria, including the one that is the focus of this study, in the open ocean. Although 80 percent of Earth's atmosphere is nitrogen, most organisms cannot use it unless it is "fixed" to other elements to make molecules like ammonia and nitrate. Because nitrogen is essential for all forms of life, nitrogen fixation is a major factor controlling overall biological productivity in the oceans.
The new microbe is one of the most abundant nitrogen fixers in many parts of the ocean, Zehr said. New DNA sequencing technology provided by 454 Life Sciences, a Roche company, enabled rapid sequencing of the organism's genome. The results showed that it is missing the entire set of genes needed for photosystem II and carbon fixation, essential parts of the molecular machinery that carries out photosynthesis in plants and cyanobacteria.
"That has multiple implications," Zehr said. "It must have a 'lifestyle' that's very different from other cyanobacteria. Ecologically, it's important to understand its role in the ecosystem and how it affects the balance of carbon and nitrogen in the ocean."
During photosynthesis, photosystem II generates oxygen by splitting water molecules. Because oxygen inhibits nitrogen fixation, most nitrogen-fixing cyanobacteria only fix nitrogen at night, or do it in specialized cells. The lack of photosystem II enables the new microbe to fix nitrogen during the day, Zehr said.
But without photosynthesis, it can't take carbon dioxide from the atmosphere and convert it into sugars. So it's not clear how the new microbe feeds itself. Either it has some way of feeding on organic matter in its environment, or it lives in close association with other organisms that provide it with food, Zehr said.
"It would make a perfect symbiont because it could feed nitrogen to its host and live on the carbon provided by the host," he said.
Zehr's team was able to obtain a mostly pure sample of these unique cyanobacteria using a flow cytometer--a device that rapidly sorts individual cells based on size and color. The flow cytometer can take the cell soup from an ocean sample, classify each cell as it passes through laser beams, and sort different cell types into separate containers. A cell type that makes up a small percentage of the original sample can be concentrated to nearly pure levels using flow cytometry.
The team harvested DNA from the concentrated sample of cyanobacteria and sequenced it using the Genome Sequencer FLX system from 454 Life Sciences. The company's new sequencing reagents and software produce extra-long "reads" of DNA, greatly increasing the speed of the sequencing process.
"We were able to gain early access to this new high-throughput technology for rapid sequencing of the microbe's genome," Zehr said. "I had begun to suspect that there was something missing in this organism's genome, and the genome sequencing confirmed that."
Photosystem II is large complex of multiple proteins and chlorophyll molecules, but the team was unable to find any of the genes for the photosystem II core proteins. The genes for photosystem I appeared in the sequencing data, as did genes for both photosystems from the small numbers of contaminating cyanobacteria in the sample.
Zehr said he plans to continue his research on the new microbe and fill some gaps in the present knowledge. Efforts are currently under way to map the microbe's presence in the oceans and determine its global abundance. Zehr is also interested in how its metabolism differs from other known cyanobacteria. If it can be cultured, there may be ways to exploit this organism's unusual metabolism in biotechnology applications, he said.
Tim Stephens | EurekAlert!
Fine organic particles in the atmosphere are more often solid glass beads than liquid oil droplets
21.04.2017 | Max-Planck-Institut für Chemie
Study overturns seminal research about the developing nervous system
21.04.2017 | University of California - Los Angeles Health Sciences
The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.
Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...
The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...
Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.
Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...
Two researchers at Heidelberg University have developed a model system that enables a better understanding of the processes in a quantum-physical experiment...
Glaciers might seem rather inhospitable environments. However, they are home to a diverse and vibrant microbial community. It’s becoming increasingly clear that they play a bigger role in the carbon cycle than previously thought.
A new study, now published in the journal Nature Geoscience, shows how microbial communities in melting glaciers contribute to the Earth’s carbon cycle, a...
20.04.2017 | Event News
18.04.2017 | Event News
03.04.2017 | Event News
21.04.2017 | Physics and Astronomy
21.04.2017 | Health and Medicine
21.04.2017 | Physics and Astronomy