Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Monell-led research identifies scent of melanoma

14.06.2013
New research may lead to early non-invasive detection and diagnosis

According to new research from the Monell Center and collaborating institutions, odors from human skin cells can be used to identify melanoma, the deadliest form of skin cancer.

In addition to detecting a unique odor signature associated with melanoma cells, the researchers also demonstrated that a nanotechnology-based sensor could reliably differentiate melanoma cells from normal skin cells. The findings suggest that non-invasive odor analysis may be a valuable technique in the detection and early diagnosis of human melanoma.

Melanoma is a tumor affecting melanocytes, skin cells that produce the dark pigment that gives skin its color. The disease is responsible for approximately 75 percent of skin cancer deaths, with chances of survival directly related to how early the cancer is detected. Current detection methods most commonly rely on visual inspection of the skin, which is highly dependent on individual self-examination and clinical skill.

The current study took advantage of the fact that human skin produces numerous airborne chemical molecules known as volatile organic compounds, or VOCs, many of which are odorous. "There is a potential wealth of information waiting to be extracted from examination of VOCs associated with various diseases, including cancers, genetic disorders, and viral or bacterial infections," notes George Preti, PhD, an organic chemist at Monell who is one of the paper's senior authors.

In the study, published online ahead of print in the Journal of Chromatography B, researchers used sophisticated sampling and analytical techniques to identify VOCs from melanoma cells at three stages of the disease as well as from normal melanocytes. All the cells were grown in culture.

The researchers used an absorbent device to collect chemical compounds from air in closed containers containing the various types of cells. Then, gas chromatography-mass spectrometry techniques were used to analyze the compounds and identified different profiles of VOCs emitting from melanoma cells relative to normal cells.

Both the types and concentrations of chemicals were affected. Melanoma cells produced certain compounds not detected in VOCs from normal melanocytes and also more or less of other chemicals. Further, the different types of melanoma cells could be distinguished from one another.

Noting that translation of these results into the clinical diagnostic realm would require a reliable and portable sensor device, the researchers went on to examine VOCs from normal melanocytes and melanoma cells using a previously described nano-sensor.

Constructed of nano-sized carbon tubes coated with strands of DNA, the tiny sensors can be bioengineered to recognize a wide variety of targets, including specific odor molecules. The nano-sensor was able to distinguish differences in VOCs from normal and several different types of melanoma cells.

"We are excited to see that the DNA-carbon nanotube vapor sensor concept has potential for use as a diagnostic. Our plan is to move forward with research into skin cancer and other diseases," said A.T. Charlie Johnson, PhD, Professor of Physics at the University of Pennsylvania, who led the development of the olfactory sensor.

Together, the findings provide proof-of-concept regarding the potential of the two analytical techniques to identify and detect biomarkers that distinguish normal melanocytes from different melanoma cell types.

"This study demonstrates the usefulness of examining VOCs from diseases for rapid and noninvasive diagnostic purposes," said Preti. "The methodology should also allow us to differentiate stages of the disease process."

Current studies are focusing on analysis of VOCs from tumor sites of patients diagnosed with primary melanoma.

Also contributing to the research were lead author Jae Kwak, Michelle Gallagher, Mehmet Hakan Ozdener, Charles J. Wysocki, Adam Faranda, and Amaka Isamah, all from Monell; A. T. Charlie Johnson, Brett R. Goldsmith, and Steven S. Fakharzadeh from the University of Pennsylvania; and Meenhard Herlyn from The Wistar Institute. Research reported in the publication was supported by The National Institute on Deafness and Other Communication Disorders of the National Institutes of Health under Award Number T 32 DC00014-26 to Monell.

The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Additional funds were donated to the Monell Center by Ms. Bonnie Hunt in memory of her parents, Ida and Percy Hunt. Support for Drs Johnson and Goldsmith came from the University of Pennsylvania Nano/Bio Interface Center through National Science Foundation grant NSEC DMR08-32802.

The Monell Chemical Senses Center is an independent nonprofit basic research institute based in Philadelphia, Pennsylvania. For 45 years, Monell has advanced scientific understanding of the mechanisms and functions of taste and smell to benefit human health and well-being. Using an interdisciplinary approach, scientists collaborate in the programmatic areas of sensation and perception; neuroscience and molecular biology; environmental and occupational health; nutrition and appetite; health and well-being; development, aging and regeneration; and chemical ecology and communication. For more information about Monell, visit http://www.monell.org.

Leslie Stein | EurekAlert!
Further information:
http://www.monell.org

More articles from Life Sciences:

nachricht New risk factors for anxiety disorders
24.02.2017 | Julius-Maximilians-Universität Würzburg

nachricht Stingless bees have their nests protected by soldiers
24.02.2017 | Johannes Gutenberg-Universität Mainz

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Breakthrough with a chain of gold atoms

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

Im Focus: DNA repair: a new letter in the cell alphabet

Results reveal how discoveries may be hidden in scientific “blind spots”

Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...

Im Focus: Dresdner scientists print tomorrow’s world

The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.

The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...

Im Focus: Mimicking nature's cellular architectures via 3-D printing

Research offers new level of control over the structure of 3-D printed materials

Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...

Im Focus: Three Magnetic States for Each Hole

Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".

Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Booth and panel discussion – The Lindau Nobel Laureate Meetings at the AAAS 2017 Annual Meeting

13.02.2017 | Event News

Complex Loading versus Hidden Reserves

10.02.2017 | Event News

International Conference on Crystal Growth in Freiburg

09.02.2017 | Event News

 
Latest News

Stingless bees have their nests protected by soldiers

24.02.2017 | Life Sciences

New risk factors for anxiety disorders

24.02.2017 | Life Sciences

MWC 2017: 5G Capital Berlin

24.02.2017 | Trade Fair News

VideoLinks
B2B-VideoLinks
More VideoLinks >>>