A team of researchers at the University of Wisconsin-Madison has identified the genes and enzymes that create a promising compound — the 19 carbon furan-containing fatty acid (19Fu-FA). The compound has a variety of potential uses as a biological alternative for compounds currently derived from fossil fuels.
Researchers from the Great Lakes Bioenergy Research Center (GLBRC), which is headquartered at UW-Madison and funded by the U.S. Department of Energy, discovered the cellular genomes that direct 19Fu-FA’s synthesis and published the new findings Aug. 4 in the journal Proceedings of the National Academy of Sciences.
“We’ve identified previously uncharacterized genes in a bacterium that are also present in the genomes of many other bacteria,” says Tim Donohue, GLBRC director and UW-Madison bacteriology professor. “So, we are now in the exciting position to mine these other bacterial genomes to produce large quantities of fatty acids for further testing and eventual use in many industries, including the chemical and fuel industries.”
The novel 19Fu-FAs were initially discovered as “unknown” products that accumulated in mutant strains of Rhodobacter sphaeroides, an organism being studied by the GLBRC because of its ability to overproduce hydrophobic, or water-insoluble, compounds.
These types of compounds have value to the chemical and fuel industries as biological replacements for plasticizers, solvents, lubricants or fuel additives that are currently derived from fossil fuels. The team also provides additional evidence that these fatty acids are able to scavenge toxic reactive oxygen species, showing that they could be potent antioxidants in both the chemical industry and cells.
Cellular genomes are the genetic blueprints that define a cell’s features or characteristics with DNA. Since the first genome sequences became available, researchers have known that many cells encode proteins with unknown functions according to the instructions specified by the cell’s DNA. But without known or obvious activity, the products derived from these blueprints remained a mystery.
As time has gone on, however, researchers have realized that significant pieces of these genetic blueprints are directing the production of enzymes — proteins that allow cells to build or take apart molecules in order to survive. These enzymes, it turned out, create new and useful compounds for society.
“I see this work as a prime example of the power of genomics,” Donohue says. “It is not often that one identifies genes for a new or previously unknown compound in cells. It is an added benefit that each of these compounds has several potential uses as chemicals, fuels or even cellular antioxidants.”
A cross-disciplinary, collaborative effort between GLBRC chemists, biochemists and bacteriologists in departments at UW-Madison and Michigan State University yielded the chemical identity of the fatty acid compounds and identified the specific genes that direct their synthesis in bacteria.
“I don’t think this discovery would have been possible,” says Rachelle Lemke, the paper’s lead author and a research specialist in Donohue’s lab, “without the analytical and intellectual expertise of the members from this center.”
Tim Donohue, 608-262-4663, firstname.lastname@example.org
Tim Donohue | newswise
New photocatalyst speeds up the conversion of carbon dioxide into chemical resources
29.05.2017 | DGIST (Daegu Gyeongbuk Institute of Science and Technology)
Copper hydroxide nanoparticles provide protection against toxic oxygen radicals in cigarette smoke
29.05.2017 | Johannes Gutenberg-Universität Mainz
The world's highest gain high power laser amplifier - by many orders of magnitude - has been developed in research led at the University of Strathclyde.
The researchers demonstrated the feasibility of using plasma to amplify short laser pulses of picojoule-level energy up to 100 millijoules, which is a 'gain'...
Staphylococcus aureus is a feared pathogen (MRSA, multi-resistant S. aureus) due to frequent resistances against many antibiotics, especially in hospital infections. Researchers at the Paul-Ehrlich-Institut have identified immunological processes that prevent a successful immune response directed against the pathogenic agent. The delivery of bacterial proteins with RNA adjuvant or messenger RNA (mRNA) into immune cells allows the re-direction of the immune response towards an active defense against S. aureus. This could be of significant importance for the development of an effective vaccine. PLOS Pathogens has published these research results online on 25 May 2017.
Staphylococcus aureus (S. aureus) is a bacterium that colonizes by far more than half of the skin and the mucosa of adults, usually without causing infections....
Physicists from the University of Würzburg are capable of generating identical looking single light particles at the push of a button. Two new studies now demonstrate the potential this method holds.
The quantum computer has fuelled the imagination of scientists for decades: It is based on fundamentally different phenomena than a conventional computer....
An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.
We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...
Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.
Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...
24.05.2017 | Event News
23.05.2017 | Event News
22.05.2017 | Event News
29.05.2017 | Life Sciences
29.05.2017 | Physics and Astronomy
29.05.2017 | Statistics