The study is reported in Issue 18, Volume 54 (September 2009) of the Chinese Science Bulletin as one of the papers in a special issue about Biomedical Materials in this journal.
Cell-material interaction is a very important fundamental topic in natural science, yet is too complex to be revealed without unique research methods. Micropatterning technique, especially photolithography, a widely used technique in microelectronic industry, has recently been employed by material scientists and biologists to generate a surface with cell-adhesion contrast to control cell localization. The present study confirms that cells could be well orientated along a micropattern with cell-adhesive stripes in an adhesion-resistant background.
"While cell orientation on a micropattern is not the first observation, our work distinguishes itself by employing a PEG hydrogel instead of a PEG self assembly monolayer as background, and thus the cell adhesion contrast would be maintained for a long time, which guarantees more convenient and convincing observations," noted the corresponding author Jian-dong DING, director of the Key Laboratory of Molecular Engineering of Polymers of the Chinese Ministry of Education and professor of the Department of Macromolecular Science, Fudan University. "This paper further put forward five statistical parameters which describe cell orientation from different aspects."
In this paper, the authors prepared, by the photolithographic transfer technique, stable gold (Au) micropatterns on PEG hydrogel surfaces with defined cell-resistant (PEG hydrogel) and cell-adhesive (gold microstripes) properties. 3T3 fibroblasts were cultured on Au-microstripe surfaces to observe cell adhesion and orientation. Five statistical parameters were defined and used to describe cell orientation on micropatterns. With the increase of inter-stripe distance, the orientational order parameter, the ratio of long and short axes of a cell, and the occupation fraction of cells on stripes increased gradually, whereas the spreading area of a single cell decreased. The abrupt changes of these four parameters did not happen at the same inter-distance. The adhesion ratio of a cell on Au stripes over cell spreading area did not change monotonically as a function of inter-stripe distance. The combination of the five statistical parameters represented well the cell orientation behaviors semi-quantitatively.
This work was a 973 project of nanoscience and financially supported by the National Basic Research Program, and Professor Ding is the current leader of the 973 project of biomedical nanomaterials. Some early research was also partially supported by Key Project of the Chinese Ministry of Education, and Science and Technology Developing Foundation of Shanghai. The first and second authors, Jian-guo SUN and Jian TANG among the three authors of this paper are PhD students of Professor DING.
The present work is ready to be extended by developing pertinent micropatterning technique in three dimensions in Material Science, examination of various kinds of cells in Biology, exploring the underling mechanism of signal transduction in Cell Biology and Molecular Biology, application of the research in Regenerative Medicine such as Tissue Engineering which is related to various surgeries.
Reference: Jianguo Sun, Jian Tang, Jiandong Ding*. Cell orientation on a stripe-micropatterned surface. Chn. Sci. Bull. 2009; 54(18): 3154-3159. http://www.wjgnet.com/1007-9327/13/4873.asp
Jiandong DING | EurekAlert!
A novel socio-ecological approach helps identifying suitable wolf habitats
17.02.2017 | Universität Zürich
New, ultra-flexible probes form reliable, scar-free integration with the brain
16.02.2017 | University of Texas at Austin
In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport
Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...
The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.
The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...
Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...
Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".
Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...
13.02.2017 | Event News
10.02.2017 | Event News
09.02.2017 | Event News
20.02.2017 | Materials Sciences
20.02.2017 | Health and Medicine
20.02.2017 | Health and Medicine