Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Microfluidics: Bubble power

15.03.2012
The latest microfluidic chip can generate microbubbles to break open cells for biochemical analysis

Scientists have made many important discoveries in biology and medicine through studying the internal contents of cells. Some have isolated or identified nucleic acids or proteins with special functions, while others have unravelled the working and regulatory mechanisms underlying biochemical or pharmaceutical components within cells.


Micrographs of GFP-expressing bacteria before and after lysis

Dave Ow and co-workers at the A*STAR Bioprocessing Technology Institute and Institute of High Performance Computing have now developed a novel method to expose the internal contents of cells for biochemical analysis1.

Currently there is a wide range of methods to disintegrate or lyse cell membranes and to release the biomolecules contained within. However, most of these methods can cause denaturation of proteins or interfere with subsequent assaying. Ow and co-workers explored the possibility of using ultrasound in microfluidics to lyse cells. They applied short bursts of ultrasound with periods of rest to prevent the proteins from overheating as a result of dissipation of mechanical energy.

When the rapid changes of pressure generated with ultrasound are applied to a liquid, small bubbles are formed which oscillate in size and generate a cyclic shear stress. These rapidly oscillating bubbles generate a mini shockwave when they implode, which can be strong enough to cause the cell membrane to rupture. The researchers generated microbubbles in the meandering microfluidic channel by introducing a gas via a separate inlet to generate a gas–liquid interface and subsequently applying ultrasound to the system.

As a proof of principle, the researchers tested the performance of their microfluidic device on genetically engineered bacteria and yeast that express the green fluorescent protein. The researchers found that the bacteria are completely disintegrated after only 0.4 seconds of ultrasound exposure (see image). The concentration of DNA released from yeast cells reached a plateau after only one second exposure (which contained six bursts of ultrasound each of 0.154 seconds), indicating that most cells are successfully lysed. Importantly the temperature of the sample was shown not to rise above 3.3 °C. “The large surface to volume ratio of the microfluidic environment means that the small amount of heat that is generated rapidly diffuses away,” says Ow.

The researchers have proposed many ideas for applications. “In collaboration with another institute, we are developing a rapid and sensitive label-free optical method for on-chip detection of bioanalytes from lysed cells,” says Ow. “We also want to modify the device to break more difficult-to-lyse endospores, and to develop a rapid on-chip detection device to counter the threats of bioterrorism.”

The A*STAR-affiliated researchers contributing to this research are from the Bioprocessing Technology Institute and Institute of High Performance Computing.

Lee Swee Heng | Research asia research news
Further information:
http://www.research.a-star.edu.sg
http://www.researchsea.com

More articles from Life Sciences:

nachricht Ion treatments for cardiac arrhythmia — Non-invasive alternative to catheter-based surgery
20.01.2017 | GSI Helmholtzzentrum für Schwerionenforschung GmbH

nachricht Seeking structure with metagenome sequences
20.01.2017 | DOE/Joint Genome Institute

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Traffic jam in empty space

New success for Konstanz physicists in studying the quantum vacuum

An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...

Im Focus: How gut bacteria can make us ill

HZI researchers decipher infection mechanisms of Yersinia and immune responses of the host

Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

Im Focus: Designing Architecture with Solar Building Envelopes

Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.

As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Sustainable Water use in Agriculture in Eastern Europe and Central Asia

19.01.2017 | Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

 
Latest News

Helmholtz International Fellow Award for Sarah Amalia Teichmann

20.01.2017 | Awards Funding

An innovative high-performance material: biofibers made from green lacewing silk

20.01.2017 | Materials Sciences

Ion treatments for cardiac arrhythmia — Non-invasive alternative to catheter-based surgery

20.01.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>