Close collaboration between four laboratories at the BIMSB led by Stefan Kempa, Christoph Dieterich, Nikolaus Rajewsky and Wei Chen has led to the identification of thousands of gene products, many of which are expressed and are important in stem cell function. This was achieved by precise characterization of all RNA-molecules expressed in the animals’ cells, the so-called transcriptome, without using the genome sequence (Genome Research, July 2011 21: 1193-1200)*.
Planarians are famous for their almost unlimited ability to regenerate any tissue via pluripotent adult stem cells. Their spectacular regenerative capabilities have been studied for more than 100 years. With the development of new molecular and genetics approaches, planarians have recently re-emerged as a model system for the study of regeneration and stem cells.
The scientists at the BIMSB combined two existing and complementary sequencing methods to decipher the transcriptome of the planarian Schmidtea mediterranea without depending on genome sequences. Their approach is of great practical importance since the genomes of many organisms are known to be extremely difficult to assemble, even with the current sequencing technologies.
Furthermore, they also were able to identify several novel gene products (mRNAs) of which they proved that they are specifically expressed in the stem cells. It is the first proteomics study of such scale in this phylum (Platyhelminthes), as Wei Chen pointed out. The catalogue of transcripts assembled in their study, together with the identified peptides, dramatically expands and refines planarian research.
The Berlin Institute for Medical Systems Biology (BIMSB) was launched by the MDC in 2008, supported by start-up funding from the Federal Ministry of Education and Research (BMBF) and the Senate of Berlin. Medical Systems Biology focuses on molecular networks of genes and proteins, their regulation and interaction with each other and their relevance in disease processes. BIMSB works closely with research institutions and networks in Berlin and beyond, in particular with Humboldt University and Charité – Universitätsmedizin Berlin and also with New York University, USA.*De novo assembly and validation of Planaria transcriptome by massive parallel sequencing and shotgun proteomics
Barbara Bachtler | Max-Delbrück-Centrum
Cryo-electron microscopy achieves unprecedented resolution using new computational methods
24.03.2017 | DOE/Lawrence Berkeley National Laboratory
How cheetahs stay fit and healthy
24.03.2017 | Forschungsverbund Berlin e.V.
Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...
In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...
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24.03.2017 | Physics and Astronomy