The results of Dr. Jana Bogum (MDC/FMP) from the MDC research group led by Professor Walter Rosenthal and PD Dr. Enno Klußmann could be important in the future for the treatment of excessive fluid retention in patients with chronic heart failure. Using a novel approach, the researchers have also discovered a new molecular mechanism controlling water homeostasis in the kidneys (Journal of the American Society of Nephrology, doi:10.1681/ASN.2012030295)*.
Graphic of a renal cell with water channels (aquaporin-2, AQP2) in the cell (left image) and with aquaporin-2 in the plasma membrane (right image, above). For example, when a person is thirsty, the hormone AVP triggers a signaling cascade and thereby elicits the redistribution of the water channels from the cell interior to the plasma membrane. The cell then directs water from primary urine via other water channels (AQP3 and 4, Fig. on the right, the right side of the cell) into the bloodstream and tissues.
(Graphic: Enno Klußmann/ Copyright: MDC)
Renal principal cells with aquaporin-2 (blue) in the cell interior (image 1) and in the plasma membranes (image 2). The substance discovered by the MDC research group (4-acetyldiphyllin; image 3), prevents the redistribution of aquaporin-2 to the cell membrane. Nuclei are shown in green.
(Immunofluorescence microscopy: Jana Bogum, Kerstin Zühlke, Burkhard Wiesner, Jenny Eichhorst/ Copyright: MDC/FMP)
Every day around 1 500 liters of blood flow through the kidneys. Of this total volume, the kidneys initially filter 180 liters of primary urine, which they concentrate to two liters and then excrete as the final urine. A key regulatory step of the concentration mechanism is the release of the hormone AVP (arginine-vasopressin) from the brain. This hormone triggers a multi-step signaling cascade in the kidneys which affects water channels (aquaporins) and in particular aquaporin-2. “The water channels, specifically aquaporin-2, and their redistribution play a key role in the regulation of the water balance,” said Dr. Klußmann.AVP, which is released from the brain upon thirst, induces aquaporin-2 located in the renal collecting duct principal cells to redistribute from the cell interior to the plasma membrane. The renal cells can then filter out the water from the primary urine flowing past the membrane via aquaporin-2. Dr. Klußmann explained: “To keep the renal cell from bursting and the body from dehydrating, the water is directed back via another group of water channels, aquaporin 3 and 4, into the bloodstream and body tissue. In contrast to aquaporin-2, these water channels are located in another domain of the plasma membrane in the renal principal cells and stay there permanently.” Once the thirst is quenched, the levels of the hormone AVP are reduced and aquaporin-2 is shuttled back into the interior of the renal cell until it is needed again.
The researchers used “small molecules“, low molecular weight organic compounds, which penetrate well into cells. They tested 17 700 such substances in renal cells and ultimately filtered out a substance that blocks the redistribution of aquaporin-2 to the plasma membrane. The substance (4-acetyldiphyllin) prevents phosphorylation, an important biological and regulatory activation step. In particular, the compound prevents a phosphorylation reaction that is catalyzed by a protein termed protein kinase A. This protein is activated in the signaling cascade that is triggered by AVP in the renal principal cells. In the presence of 4-acetyldiphillin protein kinase A cannot add a phosphate group to aquaporin-2, with the result that the water channels can no longer redistribute to the plasma membrane.The new research findings may not only be of interest for the treatment of edema but also for the treatment of depression. Here, by contrast, medical researchers are seeking a way to shuttle aquaporin-2 to the plasma membrane of the renal principal cell, because lithium, which is often used to treat depression, prevents aquaporin-2 from redistributing to the plasma membrane, thus causing diabetes insipidus. If AVP is not released from the brain, or if the receptor for AVP in the renal cell is defective, this likewise results in diabetes insipidus, as Professor Rosenthal discovered several years ago. The affected individuals excrete 20 liters of urine every day. A similar effect, but not quite as drastic, is caused by alcohol. Drinking lots of beer causes the body to excrete large amounts of urine. The reason – alcohol prevents the brain from releasing the hormone AVP and thus prevents the redistribution of aquaporin-2 to the plasma membrane.
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