Using nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography, they have determined the 3-D structure and interactions of the histone chaperone Rtt106 down to the atomic details. The findings are published in the journal Nature.
“The interactions we described are important for gene silencing and the DNA damage response,” says senior author Georges Mer, Ph.D., a Mayo Clinic structural biologist. “This is exciting because our newfound knowledge will help us better understand these fundamental cellular processes.”
In cells, our DNA is part of a structure called chromatin, comprised of proteins, the majority called histones, which are wrapped with the DNA. Associated with the histones is another group of proteins called histone chaperones, which promote the proper assembly or disassembly of the chromatin during the times our DNA is replicated or repaired when damaged. Their dysfunction has been linked to cancer, aging and other diseases.
Mayo researchers discovered two novel domains in Rtt106 that take on this role. One, termed the homodimerization domain, allows two molecules of Rtt106 to be linked so they can cooperate in binding histones H3 and H4. The other, called the double PH domain, is responsible for sensing the acetylated lysine of H3 and further reinforces the interaction. The combined actions of the two domains of Rtt106 enable it to perform the chaperoning function efficiently and properly. This is the first time anyone has described this mode of specific association between a histone chaperone and a modified histone complex.
Other authors of the study are Dan Su, M.D., Ph.D.; Qi Hu, Ph.D.; Qing Li, Ph.D.; James Thompson, Ph.D.; Gaofeng Cui, Ph.D.; Ahmed Fazly, Ph.D.; Brian Davies, Ph.D.; Maria Victoria Botuyan, Ph.D.; and Zhiguo Zhang, Ph.D.; all of Mayo Clinic. The study was a collaborative effort between the groups of Dr. Mer and Dr. Zhang and was funded by the National Institutes of Health and Mayo Clinic.
Robert Nellis | Newswise Science News
Water forms 'spine of hydration' around DNA, group finds
26.05.2017 | Cornell University
How herpesviruses win the footrace against the immune system
26.05.2017 | Helmholtz-Zentrum für Infektionsforschung
Staphylococcus aureus is a feared pathogen (MRSA, multi-resistant S. aureus) due to frequent resistances against many antibiotics, especially in hospital infections. Researchers at the Paul-Ehrlich-Institut have identified immunological processes that prevent a successful immune response directed against the pathogenic agent. The delivery of bacterial proteins with RNA adjuvant or messenger RNA (mRNA) into immune cells allows the re-direction of the immune response towards an active defense against S. aureus. This could be of significant importance for the development of an effective vaccine. PLOS Pathogens has published these research results online on 25 May 2017.
Staphylococcus aureus (S. aureus) is a bacterium that colonizes by far more than half of the skin and the mucosa of adults, usually without causing infections....
Physicists from the University of Würzburg are capable of generating identical looking single light particles at the push of a button. Two new studies now demonstrate the potential this method holds.
The quantum computer has fuelled the imagination of scientists for decades: It is based on fundamentally different phenomena than a conventional computer....
An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.
We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...
Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.
Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...
An Australian-Chinese research team has created the world's thinnest hologram, paving the way towards the integration of 3D holography into everyday...
24.05.2017 | Event News
23.05.2017 | Event News
22.05.2017 | Event News
26.05.2017 | Life Sciences
26.05.2017 | Life Sciences
26.05.2017 | Physics and Astronomy