Now, a new screening process will simplify non-viral transfection, providing a method researchers and clinicians use to find an optimal set of biomaterials to deliver genes to cells.
Developed by William Murphy, the Harvey D. Spangler professor of biomedical engineering at the University of Wisconsin-Madison, the method gives researchers greater control over how cells react to the gene delivery mechanism. The broader implication is more nuanced, effective control over cell behavior. "We've been exploring using this concept for reprogramming of adult cells, as well as controlling differentiation of stem cell types,” Murphy says.
Murphy and his collaborators published news of their advance in the March 28, 2013 issue of Nature Scientific Reports. http://www.nature.com/srep/2013/130328/srep01567/full/srep01567.html
In a current successful approach, researchers use specialized viruses to deliver genetic material to cells. While efficient, that method also carries a greater risk of turning on unwanted genes or provoking an immune response from the body —making it less attractive for sensitive biomedical applications like controlling stem cell behavior, says Murphy.
His team has developed a process that does not rely on viruses. Rather, the researchers can grow specific calcium phosphate coatings that serve as a medium via which genetic material can be delivered to cells more efficiently. By matching a coating to a specific application for delivering genes, Murphy has seen up to a 70-fold increase in successful expression of those genes in human stem cells.
“From an application standpoint, the advance could be really impactful, and could enable gene delivery to become an integral part of medical device design and tissue engineering applications,” says Murphy.
The process could be critical to further advances in regenerative medicine. Since researchers can apply it to any size or shape of tissue engineering structure, it could help provide engineers a simpler way to build the complex tissue structures required to deliver next-generation drug screening and patient therapies.
The advance was made possible with funding support from the AO Foundation and the National Institutes of Health.
—Mark Riechers, 608-265-8592, firstname.lastname@example.org
Mark Riechers | Newswise
127 at one blow...
18.01.2017 | Stiftung Zoologisches Forschungsmuseum Alexander Koenig, Leibniz-Institut für Biodiversität der Tiere
How gut bacteria can make us ill
18.01.2017 | Helmholtz-Zentrum für Infektionsforschung
Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...
Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.
While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...
Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales
Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...
Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.
As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...
At TU Wien, an alternative for resource intensive formwork for the construction of concrete domes was developed. It is now used in a test dome for the Austrian Federal Railways Infrastructure (ÖBB Infrastruktur).
Concrete shells are efficient structures, but not very resource efficient. The formwork for the construction of concrete domes alone requires a high amount of...
10.01.2017 | Event News
09.01.2017 | Event News
05.01.2017 | Event News
18.01.2017 | Materials Sciences
18.01.2017 | Information Technology
18.01.2017 | Ecology, The Environment and Conservation