Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

ISU researchers develop hybrid protein tools for gene cutting and editing

31.08.2010
An Iowa State University team of researchers has developed a type of hybrid proteins that can make double-strand DNA breaks at specific sites in living cells, possibly leading to better gene replacement and gene editing therapies.

Bing Yang, assistant professor of genetics, development and cell biology, and his colleagues developed the hybrid protein by joining parts of two different bacterial proteins. One is called a TAL effector, which functions to find the specific site on the gene that needs to be cut, and the other is an enzyme called a nuclease that cuts the DNA strands.

Yang hopes the research will lead to the ability to modify genomes by cutting out defective or undesirable parts of DNA, or by replacing defective or undesirable gene segments with a functioning piece of replacement DNA - a process called homologous recombination.

Yang says that his hybrid proteins can be constructed to locate specific segments of the DNA in any type of organism.

"This breakthrough could eventually make it possible to efficiently modify plant, animal and even human genomes," said Yang. "It should be effective in a range of organisms."

The proteins work by binding onto the specific segment of DNA the researcher wants to change. Yang's proteins do this by reading the DNA sequence and finding the specific area to be cut.

Once the protein binds onto the DNA at the correct spot, the other half of Yang's protein then cuts the double-stranded DNA.

Bad or undesirable DNA can be resected (removed) and good or more desirable DNA can be introduced. When the DNA heals, the good DNA is included in the gene.

Yang started his research about a year ago after seeing the results of research by Adam Bogdanove, ISU associate professor of plant pathology, showing that TAL effectors use a very straightforward code to bind to a specific DNA sequence.

This discovery allowed Yang to predict exactly where the TAL effector nuclease will bind on the DNA to make the cut.

Another study had similar results.

The concept has also been proven by Bogdanove and Dan Voytas, collaborator in genetics, development and cell biology at Iowa State, and director of the Center for Genome Engineering at the University of Minnesota, Twin Cities.

The TAL effector-nuclease approach improves on tools currently available for genome modification. It should be faster and less expensive to make TAL effector nucleases, and easier to design them to recognize specific DNA sequences, according to Yang.

Yang's findings recently appeared in the online version of the journal Nucleic Acids Research. Voytas' and Bogdanove's study also appeared recently in the journal Genetics.

Voytas and Bogdanove were also able to show that the TAL effector part of the hybrid protein can be customized to target new DNA sequences.

Yang's team includes Ting Li, graduate assistant; Sheng Huang, post doctoral researcher; David Wright, associate scientist; and Martin Spalding, professor and chair; all of the genetics, development and cell biology department at Iowa State; Wen Zhi Jiang, research associate; and Donald Weeks, professor; both from the University of Nebraska, Lincoln.

Bing Yang | EurekAlert!
Further information:
http://www.iastate.edu

Further reports about: Bogdanove DNA DNA sequence DNA strand ISU Iowa TAL Yang bacterial protein cell biology human genome

More articles from Life Sciences:

nachricht Cryo-electron microscopy achieves unprecedented resolution using new computational methods
24.03.2017 | DOE/Lawrence Berkeley National Laboratory

nachricht How cheetahs stay fit and healthy
24.03.2017 | Forschungsverbund Berlin e.V.

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Argon is not the 'dope' for metallic hydrogen

24.03.2017 | Materials Sciences

Astronomers find unexpected, dust-obscured star formation in distant galaxy

24.03.2017 | Physics and Astronomy

Gravitational wave kicks monster black hole out of galactic core

24.03.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>