Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Insights into a Virus Proteome

23.11.2012
Max Planck Scientists Identify Unknown Proteins of the Herpesvirus

The genome encodes the complete information needed by an organism, including that required for protein production. Viruses, which are up to a thousand times smaller than human cells, have considerably smaller genomes.


Herpesvirus

Using a type of herpesvirus as a model system, the scientists of the Max Planck Institute (MPI) of Biochemistry in Martinsried near Munich and their collaboration partners at the University of California in San Francisco have shown that the genome of this virus contains much more information than previously assumed.

The researchers identified several hundred novel proteins, many of which were surprisingly small. The results of the study have now been published in Science.

More than 80 percent of the world’s population is infected with the herpesvirus, which can cause severe diseases in newborns and in persons with weakened immune system. Researchers had already sequenced the herpesvirus genome 20 years ago, thinking they could then predict all proteins that the virus produces (virus proteome). Now scientists from the research department of Matthias Mann, director at the MPI of Biochemistry, and their American colleagues have analyzed the information content of the genome more precisely.

Small but highly complex

To carry out their study, the scientists infected cells with herpesvirus and observed which proteins the virus produced inside the cell over a period of 72 hours. In order for proteins to be produced at all, the cell machinery must first make copies of the genetic material as intermediate products (RNA). While investigating the intermediate products of the herpesvirus, the American collaborators discovered many novel RNA molecules which were in large part surprisingly short. They also found that the organization of information required for protein production in the virus genome was far more complex than previously assumed. Annette Michalski, a scientist in the Department of Proteomics and Signal Transduction at the MPI of Biochemistry, was subsequently able to confirm directly the predicted viral proteins in the infected cell using mass spectrometry. This method enables an overview of the complete proteome of the virus-infected cell.

The results of the American and German researchers provide detailed insight into the complex mechanisms used by the virus. “We showed that it’s not enough merely to know the virus genome to understand the biology of the herpesvirus,” Annette Michalski said. “What is important is to look at the products actually produced from the genome.” Even human genes may be much more complex than the genome sequence itself indicates, commented the researchers. Matthias Mann and his colleagues plan to investigate this question further in the coming years.

Original publication:
N. Stern-Ginossar , B. Weisburd, A. Michalski, V. T. Khanh Le, M. Y. Hein, S.-X. Huang, M. Ma, B. Shen, S.-B. Qian, H. Hengel, M. Mann, N. T. Ingolia, J. S. Weissmann: Decoding Human Cytomegalovirus, Science, November 23, 2012.
DOI:10.1126/science.1227919

Contact:
Prof. Dr. Matthias Mann
Proteomics and Signal Transduction
Max Planck Institute of Biochemistry
Am Klopferspitz 18
82152 Martinsried
Germany
e-mail: mmann@biochem.mpg.de
www.biochem.mpg.de/mann

Anja Konschak
Public Relations
Max Planck Institute of Biochemistry
Am Klopferspitz 18
82152 Martinsried
Germany
Phone: +49 89 8578-2824
e-mail: konschak@biochem.mpg.de
Further information:

http://www.biochem.mpg.de/en/news/pressroom/086_Mann_HCMV.html

http://www.biochem.mpg.de/mann
(Research Department Proteomics and Signal Transduction)

http://weissmanlab.ucsf.edu/
Hompage of Weissmann Lab at UCSF)

Anja Konschak | Max-Planck-Institut
Further information:
http://www.biochem.mpg.de

More articles from Life Sciences:

nachricht Quasi-sexual gene transfer drives genetic diversity of hot spring bacteria
29.05.2015 | Carnegie Institution

nachricht Scientists use unmanned aerial vehicle to study gray whales from above
29.05.2015 | NOAA National Marine Fisheries Service

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Lasers are the key to mastering challenges in lightweight construction

Many joining and cutting processes are possible only with lasers. New technologies make it possible to manufacture metal components with hollow structures that are significantly lighter and yet just as stable as solid components. In addition, lasers can be used to combine various lightweight construction materials and steels with each other. The Fraunhofer Institute for Laser Technology ILT in Aachen is presenting a range of such solutions at the LASER World of Photonics trade fair from June 22 to 25, 2015 in Munich, Germany, (Hall A3, Stand 121).

Lightweight construction materials are popular: aluminum is used in the bodywork of cars, for example, and aircraft fuselages already consist in large part of...

Im Focus: Solid-state photonics goes extreme ultraviolet

Using ultrashort laser pulses, scientists in Max Planck Institute of Quantum Optics have demonstrated the emission of extreme ultraviolet radiation from thin dielectric films and have investigated the underlying mechanisms.

In 1961, only shortly after the invention of the first laser, scientists exposed silicon dioxide crystals (also known as quartz) to an intense ruby laser to...

Im Focus: Advance in regenerative medicine

The only professorship in Germany to date, one master's programme, one laboratory with worldwide unique equipment and the corresponding research results: The University of Würzburg is leading in the field of biofabrication.

Paul Dalton is presently the only professor of biofabrication in Germany. About a year ago, the Australian researcher relocated to the Würzburg department for...

Im Focus: Basel Physicists Develop Efficient Method of Signal Transmission from Nanocomponents

Physicists have developed an innovative method that could enable the efficient use of nanocomponents in electronic circuits. To achieve this, they have developed a layout in which a nanocomponent is connected to two electrical conductors, which uncouple the electrical signal in a highly efficient manner. The scientists at the Department of Physics and the Swiss Nanoscience Institute at the University of Basel have published their results in the scientific journal “Nature Communications” together with their colleagues from ETH Zurich.

Electronic components are becoming smaller and smaller. Components measuring just a few nanometers – the size of around ten atoms – are already being produced...

Im Focus: IoT-based Advanced Automobile Parking Navigation System

Development and implementation of an advanced automobile parking navigation platform for parking services

To fulfill the requirements of the industry, PolyU researchers developed the Advanced Automobile Parking Navigation Platform, which includes smart devices,...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International symposium: trends in spatial analysis and modelling for a more sustainable land use

20.05.2015 | Event News

15th conference of the International Association of Colloid and Interface Scientists

18.05.2015 | Event News

EHFG 2015: Securing health in Europe. Balancing priorities, sharing responsibilities

12.05.2015 | Event News

 
Latest News

Quasi-sexual gene transfer drives genetic diversity of hot spring bacteria

29.05.2015 | Life Sciences

First Eastern Pacific tropical depression runs ahead of dawn

29.05.2015 | Earth Sciences

Donuts, math, and superdense teleportation of quantum information

29.05.2015 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>