Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

In vitro tests of Vype vapor reveal no cell stress, DNA damage or cell transformation

15.03.2016

In each test, Vype ePen, a commercially available e-cigarette, produced the same results as an untreated control -- there was no activity

A series of cell-based tests developed to compare the biological impact of cigarette smoke with e-cigarette vapour revealed no activity in cells exposed to vapour from Vype ePen, a commercially available e-cigarette. In contrast, when the cell culture systems were exposed to cigarette smoke, they exhibited a series of responses including stress responses, DNA damage and cellular transformation, depending on the assay used.


Vype vapor produces the same result as an untreated control -- no activity in tests for cellular stress, double-strand DNA break and tumor promotion.

Credit: British American Tobacco

The use of these tests to assess the biological impact of e-cigarettes was reported by scientists from British American Tobacco at the annual meeting of the Society of Toxicology in New Orleans today.

'The results of these tests show that toxicity and biological activity is unaffected by the vapour from the e-cigarette tested, Vype ePen,' said Dr Kevin McAdam, Head of Next Generation Product (NGP) Research at British American Tobacco. 'These tests are part of a suite of tests being developed to test novel tobacco and nicotine products and could be used to help develop standards for these products in the future,' he said.

E-cigarette vapour can contain nicotine, humectants, flavourings and thermal degradation products, so it is important to understand the potential impact on biological systems.

A number of tests were used to compare the biological impact of cigarette smoke and e-cigarette vapour: cell stress tests, looking at the production of intracellular antioxidants, free radicals and inflammatory markers; assessment of DNA damage, which can set the scene for cancer; and a transformation assay, which measures the transformation or conversion of normal cells into a cancerous cell phenotype.

Stress

Cells respond to stress in a number of ways. They can produce compounds that protect the cellular structures or they can recruit compounds from the immune system to help protect the cell or commit suicide.

By measuring the levels of the various compounds produced and the level of cell apoptosis/death, it is possible to determine the levels of cellular stress.

The cell culture systems tested conventional 3R4F reference cigarette and Vype ePen, a commercially available e-cigarette. When cells were exposed to the cigarette smoke, all cell stress responses were activated. These same cell stress responses were not activated on exposure to e-cigarette vapour.

Damage

Cellular DNA can become damaged by exposure to toxicants, especially when stressed. DNA double-strand breaks (DSB) in which both strands of the double helix are broken, is the most serious type of DNA damage. This is a possible precursor to cancer and potentially lethal to the cell. The cell attempts to repair the DNA damage by modifying the histone or protein around which the DNA is wrapped. The changes observed in this histone can be used as an indicator of the level of DSB.

When this test was used to compare the impact of conventional 3R4F reference cigarette and Vype ePen on DSBs, the results showed that cigarette smoke induced significant DNA damage in human lung cells. This was dose dependent, that is, the higher the dose, the more DNA damage was induced. E-cigarette vapour produced no affect, even when the dose used was 15 times higher than the equivalent smoke exposure.

Disease

Damaged cells often go on to become cancerous. The cells are transformed from normal cells to abnormal cells that clump together and grow uncontrollably, eventually becoming tumour-like. This process can be mimicked in the lab by using cells that are already damaged and testing the tumour-promoting activities of different compounds.

In this case, the cell culture system was used to test the ability of conventional 3R4F reference cigarette and Vype ePen to promote tumour formation in a specialised cell type called Bhas 42.

After exposure to reference cigarettes, the layers of cells were seen to become transformed, clumping together to create colonies, suggesting that the smoke is a tumour promoter. By contrast, the e-cigarette produced no activity.

In each test, the e-cigarette produced the same results as an untreated control - there was no activity.

Many in the public health community believe that e-cigarettes are substantially reduced risk compared to cigarettes. Public Health England, an executive body of the UK Department of Health, recently published a report saying that e-cigarettes are 95% safer than cigarettes. But there are still no internationally agreed testing protocols to establish this.

Media Contact

Marina Murphy
marina_murphy@bat.com
44-077-111-50135

 @BAT_Sci

http://www.bat-science.com 

Marina Murphy | EurekAlert!

Further reports about: DNA DNA damage Tobacco cell stress cigarette smoke culture e-cigarette e-cigarettes normal cells

More articles from Life Sciences:

nachricht Symbiotic bacteria: from hitchhiker to beetle bodyguard
28.04.2017 | Johannes Gutenberg-Universität Mainz

nachricht Nose2Brain – Better Therapy for Multiple Sclerosis
28.04.2017 | Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Making lightweight construction suitable for series production

More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.

Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...

Im Focus: Wonder material? Novel nanotube structure strengthens thin films for flexible electronics

Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.

"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...

Im Focus: Deep inside Galaxy M87

The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.

Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...

Im Focus: A Quantum Low Pass for Photons

Physicists in Garching observe novel quantum effect that limits the number of emitted photons.

The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...

Im Focus: Microprocessors based on a layer of just three atoms

Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.

Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Fighting drug resistant tuberculosis – InfectoGnostics meets MYCO-NET² partners in Peru

28.04.2017 | Event News

Expert meeting “Health Business Connect” will connect international medical technology companies

20.04.2017 | Event News

Wenn der Computer das Gehirn austrickst

18.04.2017 | Event News

 
Latest News

Wireless power can drive tiny electronic devices in the GI tract

28.04.2017 | Medical Engineering

Ice cave in Transylvania yields window into region's past

28.04.2017 | Earth Sciences

Nose2Brain – Better Therapy for Multiple Sclerosis

28.04.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>