Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Immunologists of Mainz University present improved mass spectrometric method for proteomic analyses

17.03.2014

Breakthrough discovery offers new perspectives for research on the immune and nervous system / Publication in Nature Methods

When it comes to analyzing cell components or body fluids or developing new medications, there is no way around mass spectrometry. Mass spectrometry is a highly sensitive method of measurement that has been used for many years for the analysis of chemical and biological materials.

Scientists at the Institute of Immunology of the University Medical Center of Johannes Gutenberg University Mainz (JGU) have now significantly improved this analytical method that is widely employed within their field. They have also developed a software program for the integrated analysis of measurement data called ISOQuant.

Their optimized mass spectrometric workflow allows to identify and quantify significantly more proteins than before. The development of this enhanced method of measurement and the specially designed software is described in an article recently published in the prestigious journal Nature Methods.

A proteome represents the entire set of proteins expressed by a cell. Through analysis of proteomes, it is thus possible to obtain a comprehensive picture of the proteins and peptides present in cells or body fluids. However, many of the traditional mass spectrometric methods used to date for proteomic analysis are relatively slow and do not always provide reproducible results.

Dr. Stefan Tenzer of the Institute of Immunology and his colleagues have perfected a relatively new, data-independent technique that facilitates a very accurate and reproducible quantitative analysis. With its help, many more proteins can be identified than before. "Figuratively speaking, the equipment we use is as exact as a scale that can tell whether a two-euro coin is present in a VW Beetle or not," explains Tenzer.

Tenzer's work group focuses in particular on developing novel techniques for quantitative proteomic analysis with the aid of so-called ion mobility mass spectrometry. This technique allows not only to measure the mass of a molecule but can also to determine its cross section.

This additional analytical dimension renders the technique optimally suited for the comprehensive investigation of highly complex samples. Tenzer and his colleagues have also managed to enhance the technique known as label-free quantification. This eliminates the need for samples to be labeled in the laboratory before being analyzed, an otherwise complex procedure.

"We are now able to directly analyze patient samples and specific immune cells without prior cost-intensive preparation," says Tenzer. The Mainz-based scientists specifically developed their ISOQuant software program for this purpose. This provides for standardized analysis of complex data material and generally simplifies the technique of quantitative mass spectrometric analysis. 

These groundbreaking innovations were developed under the aegis of the technology platforms "Quantitative Proteomic Analysis" of the JGU Research Center Immunology (FZI) and "ProTIC" of the Research Unit Translational Neurosciences (FTN) at the Mainz University Medical Center. They were now published in Nature Methods, one of the most respected international journals. This was already the third article published in the Nature journal group in 2013 by Dr. Stefan Tenzer and his colleagues.

"The years of work within the technology platform have paid off in terms of a quantum leap forward with regard to the improvement of the technique of proteomic analysis mass spectrometry," stated Professor Hansjörg Schild, Director of the Institute of Immunology and Coordinator of the Research Center Immunology (FZI) at the Mainz University Medical Center. "The results obtained by Dr. Stefan Tenzer and his colleagues reflect the quality of achievement of this team. I think we can look forward to new and exciting collaborations in future," said Schild. 

"Mass spectrometry is a technique that has now become indispensable within the field of the neurosciences. In this area, we specifically need highly sensitive analytical techniques and Dr. Tenzer has opened up new perspectives in this regard," emphasized Professor Robert Nitsch, Coordinator of the Research Unit Translational Neurosciences and of the Collaborative Research Center 1080 on "Molecular and Cellular Mechanisms of Neuronal Homeostasis" at the Mainz University Medical Center. "The collaboration between the Research Center Immunology and the Research Unit Translational Neurosciences in the field of mass spectrometry represents an excellent opportunity for us to gain new insights into the way the brain functions," claimed Nitsch.

Publication:
Ute Distler, Jörg Kuharev, Pedro Navarro, Yishai Levin, Hansjörg Schild & Stefan Tenzer, “Drift time-specific collision energies enable deep-coverage data- independent acquisition proteomics“,15 December 2013
doi:10.1038/nmeth.2767
http://www.nature.com/nmeth/journal/vaop/ncurrent/index.html

Contact:
Dr. Stefan Tenzer
Head of the Core Facility for Mass Spectrometry
Institute of Immunology
Mainz University Medical Center
D 55131 Mainz
phone +49 6131 17-6199
fax +49 6131 17-6202
e-mail: tenzer@uni-mainz.de
http://www.uni-mainz.de/FB/Medizin/immunologie/neu/en/mitarbeiter-info/Stefan-Te...

Professor Dr. Hansjörg Schild
Director of the Institute of Immunology and Coordinator of the Research Center Immunology Mainz University Medical Center
D 55131 Mainz
phone +49 6131 39-32401
fax +49 6131 39-35688
e-mail: schild@uni-mainz.de
http://www.uni-mainz.de/FB/Medizin/immunologie/neu/en/mitarbeiter-info/Hansjörg-Schild/54

Weitere Informationen:

http://www.nature.com/nmeth/journal/vaop/ncurrent/index.html - publication

Petra Giegerich | idw - Informationsdienst Wissenschaft

More articles from Life Sciences:

nachricht Antimicrobial substances identified in Komodo dragon blood
23.02.2017 | American Chemical Society

nachricht New Mechanisms of Gene Inactivation may prevent Aging and Cancer
23.02.2017 | Leibniz-Institut für Alternsforschung - Fritz-Lipmann-Institut e.V. (FLI)

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Breakthrough with a chain of gold atoms

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

Im Focus: DNA repair: a new letter in the cell alphabet

Results reveal how discoveries may be hidden in scientific “blind spots”

Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...

Im Focus: Dresdner scientists print tomorrow’s world

The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.

The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...

Im Focus: Mimicking nature's cellular architectures via 3-D printing

Research offers new level of control over the structure of 3-D printed materials

Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...

Im Focus: Three Magnetic States for Each Hole

Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".

Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Booth and panel discussion – The Lindau Nobel Laureate Meetings at the AAAS 2017 Annual Meeting

13.02.2017 | Event News

Complex Loading versus Hidden Reserves

10.02.2017 | Event News

International Conference on Crystal Growth in Freiburg

09.02.2017 | Event News

 
Latest News

From rocks in Colorado, evidence of a 'chaotic solar system'

23.02.2017 | Physics and Astronomy

'Quartz' crystals at the Earth's core power its magnetic field

23.02.2017 | Earth Sciences

Antimicrobial substances identified in Komodo dragon blood

23.02.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>