Breakthrough discovery offers new perspectives for research on the immune and nervous system / Publication in Nature Methods
When it comes to analyzing cell components or body fluids or developing new medications, there is no way around mass spectrometry. Mass spectrometry is a highly sensitive method of measurement that has been used for many years for the analysis of chemical and biological materials.
Scientists at the Institute of Immunology of the University Medical Center of Johannes Gutenberg University Mainz (JGU) have now significantly improved this analytical method that is widely employed within their field. They have also developed a software program for the integrated analysis of measurement data called ISOQuant.
Their optimized mass spectrometric workflow allows to identify and quantify significantly more proteins than before. The development of this enhanced method of measurement and the specially designed software is described in an article recently published in the prestigious journal Nature Methods.
A proteome represents the entire set of proteins expressed by a cell. Through analysis of proteomes, it is thus possible to obtain a comprehensive picture of the proteins and peptides present in cells or body fluids. However, many of the traditional mass spectrometric methods used to date for proteomic analysis are relatively slow and do not always provide reproducible results.
Dr. Stefan Tenzer of the Institute of Immunology and his colleagues have perfected a relatively new, data-independent technique that facilitates a very accurate and reproducible quantitative analysis. With its help, many more proteins can be identified than before. "Figuratively speaking, the equipment we use is as exact as a scale that can tell whether a two-euro coin is present in a VW Beetle or not," explains Tenzer.
Tenzer's work group focuses in particular on developing novel techniques for quantitative proteomic analysis with the aid of so-called ion mobility mass spectrometry. This technique allows not only to measure the mass of a molecule but can also to determine its cross section.
This additional analytical dimension renders the technique optimally suited for the comprehensive investigation of highly complex samples. Tenzer and his colleagues have also managed to enhance the technique known as label-free quantification. This eliminates the need for samples to be labeled in the laboratory before being analyzed, an otherwise complex procedure.
"We are now able to directly analyze patient samples and specific immune cells without prior cost-intensive preparation," says Tenzer. The Mainz-based scientists specifically developed their ISOQuant software program for this purpose. This provides for standardized analysis of complex data material and generally simplifies the technique of quantitative mass spectrometric analysis.
These groundbreaking innovations were developed under the aegis of the technology platforms "Quantitative Proteomic Analysis" of the JGU Research Center Immunology (FZI) and "ProTIC" of the Research Unit Translational Neurosciences (FTN) at the Mainz University Medical Center. They were now published in Nature Methods, one of the most respected international journals. This was already the third article published in the Nature journal group in 2013 by Dr. Stefan Tenzer and his colleagues.
"The years of work within the technology platform have paid off in terms of a quantum leap forward with regard to the improvement of the technique of proteomic analysis mass spectrometry," stated Professor Hansjörg Schild, Director of the Institute of Immunology and Coordinator of the Research Center Immunology (FZI) at the Mainz University Medical Center. "The results obtained by Dr. Stefan Tenzer and his colleagues reflect the quality of achievement of this team. I think we can look forward to new and exciting collaborations in future," said Schild.
"Mass spectrometry is a technique that has now become indispensable within the field of the neurosciences. In this area, we specifically need highly sensitive analytical techniques and Dr. Tenzer has opened up new perspectives in this regard," emphasized Professor Robert Nitsch, Coordinator of the Research Unit Translational Neurosciences and of the Collaborative Research Center 1080 on "Molecular and Cellular Mechanisms of Neuronal Homeostasis" at the Mainz University Medical Center. "The collaboration between the Research Center Immunology and the Research Unit Translational Neurosciences in the field of mass spectrometry represents an excellent opportunity for us to gain new insights into the way the brain functions," claimed Nitsch.
Ute Distler, Jörg Kuharev, Pedro Navarro, Yishai Levin, Hansjörg Schild & Stefan Tenzer, “Drift time-specific collision energies enable deep-coverage data- independent acquisition proteomics“,15 December 2013
Dr. Stefan Tenzer
Head of the Core Facility for Mass Spectrometry
Institute of Immunology
Mainz University Medical Center
D 55131 Mainz
phone +49 6131 17-6199
fax +49 6131 17-6202
Professor Dr. Hansjörg Schild
Director of the Institute of Immunology and Coordinator of the Research Center Immunology Mainz University Medical Center
D 55131 Mainz
phone +49 6131 39-32401
fax +49 6131 39-35688
Petra Giegerich | idw - Informationsdienst Wissenschaft
Tiny songbird discovered to migrate non-stop, 1,500 miles over the Atlantic
01.04.2015 | University of Massachusetts at Amherst
The 'intraterrestrials': New viruses discovered in ocean depths
01.04.2015 | National Science Foundation
Spring is here and ectotherms, or animals dependent on external sources to raise their body temperature, are becoming more active. Recent studies have shown...
Glass-fronted office buildings are some of the biggest energy consumers, and regulating their temperature is a big job. Now a façade element developed by Fraunhofer researchers and designers for glass fronts is to reduce energy consumption by harnessing solar thermal energy. A demonstrator version will be on display at Hannover Messe.
In Germany, buildings account for almost 40 percent of all energy usage. Heating, cooling and ventilating homes, offices and public spaces is expensive – and...
Outstanding chemical, thermal and tribological properties predestine silicon carbide for the production of ceramic components of high volume. A novel method now overcomes the procedural and technical limitations of conventional design methods for the production of components with large differences in wall thickness and demanding undercuts.
Extremely hard as diamond, shrinking-free manufacturing, resistance to chemicals, wear and temperatures up to 1300 °C: Silicon carbide (SiSiC) bundles all...
In an experiment at the Department of Energy's SLAC National Accelerator Laboratory, scientists precisely measured the temperature and structure of aluminum as...
The IPH presents a solution at HANNOVER MESSE 2015 to make ship traffic more reliable while decreasing the maintenance costs at the same time. In cooperation with project partners, the research institute from Hannover, Germany, has developed a sensor system which continuously monitors the condition of the marine gearbox, thus preventing breakdowns. Special feature: the monitoring system works wirelessly and energy-autonomously. The required electrical power is generated where it is needed – directly at the sensor.
As well as cars need to be certified regularly (in Germany by the TÜV – Technical Inspection Association), ships need to be inspected – if the powertrain stops...
25.03.2015 | Event News
19.03.2015 | Event News
17.03.2015 | Event News
01.04.2015 | Earth Sciences
01.04.2015 | Information Technology
01.04.2015 | Physics and Astronomy