Our lungs are permanently exposed to harmful environmental factors that can damage or even destroy their cells. In a specific regenerative process these injured cells must be replaced as soon as possible. In collaboration with colleagues from the Max Planck Institute (MPI) of Biochemistry, scientists at the Helmholtz Zentrum München have now, for the first time, gained detailed insights into the dynamic remodeling of the tissue during lung repair. The results have just been published in EMBO Molecular Systems Biology.
According to the World Health Organization (WHO), lung diseases are the third most common cause of death worldwide: toxic particles, infections, and chronic inflammatory responses pose a permanent threat to our lungs.
Source: Fotolia [M] Herbert Schiller
To date, the regenerative mechanisms leading to healing of lung injury remain incompletely understood. Since few to no causal therapies are in place for most lung diseases, it is important to understand how these healing processes, which involve initial inflammation, fibrosis, and then resolution thereof, occur in the lung.
Using novel mass spectrometry techniques, an interdisciplinary team of scientists led by Prof. Matthias Mann, Director at the MPI of Biochemistry, and Prof. Oliver Eickelberg, Chairman of the Comprehensive Pneumology Center (CPC) at the Helmholtz Zentrum München and University Hospital of the Ludwig-Maximilians-Universität, has now succeeded, for the first time, to quantify and profile dynamic changes in the composition of the lung tissue throughout the different phases of lung regeneration.
More than 8,000 proteins examined
When the pulmonary alveoli are damaged, various proteins are secreted into the extracellular space, where they form the so-called extracellular matrix (ECM). These proteins are crucial for tissue healing by instructing various processes, including the activation of specific stem cell populations, ensuring that lung tissue can be restored to its original condition.
Now, the scientists succeeded for the first time in identifying and quantifying the abundance and solubility of more than 8,000 proteins in the lung proteome throughout the multistage tissue repair processes. “The information we have gained about the dynamic changes in ECM composition and its interactions with various secreted growth factor proteins enables us to develop new hypotheses for the activation of stem cells in the lung,” explains Dr. Herbert Schiller, first author of the study.
The findings of the research team will provide an important basis for further translational research on the development of pulmonary fibrosis* and chronic lung diseases in general, the scientists say. “These novel mass spectrometry techniques enable us to analyze variations in the type and abundance of proteins in patients with lung fibrosis and healthy individuals and will therefore likely lead to new approaches for the treatment of chronic lung diseases in general and lung fibrosis in particular”, Eickelberg predicts.
* Pulmonary fibrosis is a chronic lung disease, characterized by pathological accumulation of ECM proteins, which results in hardening of the lung and thus difficulties to breathe. The development of fibrosis is believed to be a result of defective regeneration mechanisms.
Schiller, HB et al. (2015) Time- and compartment-resolved proteome profiling of the extracellular niche in lung injury and repair, Molecular Systems Biology, DOI: : 10.15252/msb.20156123
As German Research Center for Environmental Health, Helmholtz Zentrum München pursues the goal of developing personalized medical approaches for the prevention and therapy of major common diseases such as diabetes mellitus and lung diseases. To achieve this, it investigates the interaction of genetics, environmental factors and lifestyle. The Helmholtz Zentrum München has about 2,300 staff members and is headquartered in Neuherberg in the north of Munich. Helmholtz Zentrum München is a member of the Helmholtz Association, a community of 18 scientific-technical and medical-biological research centers with a total of about 37,000 staff members.
The Comprehensive Pneumology Center (CPC) is a joint research project of the Helmholtz Zentrum München, the Ludwig-Maximilians-Universität Clinic Complex and the Asklepios Fachkliniken München-Gauting. The CPC's objective is to conduct research on chronic lung diseases in order to develop new diagnosis and therapy strategies. The CPC maintains a focus on experimental pneumology with the investigation of cellular, molecular and immunological mechanisms involved in lung diseases. The CPC is a site of the Deutsches Zentrum für Lungenforschung (DZL).
Contact for the media:
Department of Communication, Helmholtz Zentrum München – German Research Center for Environmental Health (GmbH), Ingolstädter Landstr. 1, 85764 Neuherberg - Phone: +49 89 3187 2238 - Fax: +49 89 3187 3324 – E-mail: firstname.lastname@example.org
Scientific contact at Helmholtz Zentrum München:
Prof. Dr. Oliver Eickelberg, Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Institut für Lungenbiologie, Ingolstädter Landstr. 1, 85764 Neuherberg - Phone: +49 89 3187 4666 - E-mail: email@example.com
http://msb.embopress.org/cgi/doi/10.15252/msb.20156123 - Link to the publication
http://www.helmholtz-muenchen.de/en/news/press-releases/2015/index.html - Press Releases of the Helmholtz Zentrum München
http://www.helmholtz-muenchen.de/en/ilbd/index.html - Institut für Lungenbiologie/Comprehensive Pneumology Center
Helmholtz Kommunikation | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt
Closing the carbon loop
08.12.2016 | University of Pittsburgh
Newly discovered bacteria-binding protein in the intestine
08.12.2016 | University of Gothenburg
In recent years, lasers with ultrashort pulses (USP) down to the femtosecond range have become established on an industrial scale. They could advance some applications with the much-lauded “cold ablation” – if that meant they would then achieve more throughput. A new generation of process engineering that will address this issue in particular will be discussed at the “4th UKP Workshop – Ultrafast Laser Technology” in April 2017.
Even back in the 1990s, scientists were comparing materials processing with nanosecond, picosecond and femtosesecond pulses. The result was surprising:...
Have you ever wondered how you see the world? Vision is about photons of light, which are packets of energy, interacting with the atoms or molecules in what...
A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.
Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...
In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.
“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...
The Max Planck Institute for Physics (MPP) is opening up a new research field. A workshop from November 21 - 22, 2016 will mark the start of activities for an innovative axion experiment. Axions are still only purely hypothetical particles. Their detection could solve two fundamental problems in particle physics: What dark matter consists of and why it has not yet been possible to directly observe a CP violation for the strong interaction.
The “MADMAX” project is the MPP’s commitment to axion research. Axions are so far only a theoretical prediction and are difficult to detect: on the one hand,...
16.11.2016 | Event News
01.11.2016 | Event News
14.10.2016 | Event News
08.12.2016 | Life Sciences
08.12.2016 | Physics and Astronomy
08.12.2016 | Materials Sciences