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High-speed imaging method captures entire brain activity


Researchers at the Institute of Molecular Pathology (IMP) and the Max F. Perutz Laboratories (MFPL) in Vienna, Austria, collaborated with scientists at the MIT to create an imaging system that reveals neural activity throughout the entire nervous system of living animals. This technique, the first that can generate 3-D movies of entire brains at the millisecond timescale, could help to discover how neuronal networks process sensory information and generate behavior. The new approach is described in an online publication by the journal Nature Methods on May 18, 2014.

The team used the new system to simultaneously image the activity of every neuron in the worm Caenorhabditis elegans, as well as the entire brain of a zebrafish larva, offering a more complete picture of nervous system activity than has been previously possible.

This image shows the head region and the majority of the brain of a zebrafish larvae, as recorded and reconstructed using the light-field microscope. IMP

A microlens array captures different perspectives of the sample, in this case a live larval zebrafish. IMP

“The new method is an indispensible tool to understand how the brain represents and processes sensory information and how this leads to cognitive functions and behaviour,” says physicist Alipasha Vaziri, a joint group leader at the IMP and MFPL and head of the research platform „Quantum Phenomena & Nanoscale Biological Systems“ (QuNaBioS) of the University of Vienna, who led the project. “Because of the enormous density of the interconnection of nerve cells in the brain, relevant information is often encoded in states of this densely interconnected network of neurons rather than in the activity of individual neurons.”

Vaziri’s team developed the brain-mapping method together with researchers in the lab of Edward Boyden, an associate professor of biological engineering and brain and cognitive sciences at the Massachusetts Institute of Technology.

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High-speed functional 3-D imaging

Neurons encode information - sensory data, motor plans, emotional states, and thoughts - using electrical impulses called action potentials, which provoke calcium ions to stream into each cell as it fires. By engineering model organisms that carry fluorescent proteins which glow when they bind calcium, scientists can visualize this electrical firing of neurons in live animals. However, until now there has been no way to image this neural activity over a large volume, in three dimensions, and at high speed.

Scanning the brain with a laser beam can produce 3-D images of neural activity, but it takes a long time to capture an image because each point must be scanned individually. The research-team wanted to achieve similar 3-D functional images but accelerate the process so they could see neuronal firing, which takes only milliseconds, as it occurs.

The new method is based on a technology known as light-field imaging, which creates 3-D images by capturing angular information of incoming rays of light. In the new paper, the researchers in Vienna and Cambridge built a light-field microscope which was optimized to have single neuron resolution and applied it, for the first time, to imaging of neural activity.

With this kind of microscope, the light emitted by the sample is sent through an array of lenses that refracts the light in different directions. Each point of the sample generates about 400 different points of light, which can then be recombined using a computer algorithm to recreate 3-D structures.

“Compared to existing methods, our new technology allows us to capture neuronal activity in volumes up to a thousand times larger at ten times higher speed”, says Robert Prevedel, a postdoc in the Vaziri Lab and first author of the paper. ”We have eliminated the need to scan multiple layers, thus the temporal resolution is only limited by the camera sensor and the properties of the molecules themselves.” Prevedel built the microscope at the IMP in Vienna. Young-Gyu Yoon, a graduate student at MIT and co-first author, devised the computational strategies that reconstruct the 3-D images.

Neurons in action

The researchers used the technique to image neural activity in the worm C. elegans, the only organism for which the entire neural wiring diagram is known. This one-millimeter worm has 302 neurons, each of which the researchers imaged as the worm performed natural behaviors, such as crawling.

To demonstrate the power of the new technology in higher organisms, they also studied larvae of zebrafish. Their nervous system consists of over 100 000 neurons that fire at a much faster rate, rather like humans. In the tiny larvae, the scientists were able to induce neuronal response to odor stimuli in around 500 neurons and track the nerve signals simultaneously in about 5000 activated neurons.

The findings could be ultimately useful in developing new types of algorithms that simulate functions of the brain and predict behaviour. Such models are in high demand in the area of machine learning and object recognition and classification.

The work in Vienna was funded by the Vienna Science and Technology Fund (WWTF), the Research Platform Quantum Phenomena and Nanoscale Biological Systems (QuNaBioS), the Human Frontiers Science Program, the European Commission, the VIPS Program of the Austrian Federal Ministry of Science and Research,the City of Vienna, and the Vienna Scientific Cluster (VSC).The IMP is funded by Boehringer Ingelheim.

Original Publication
Prevedel R, Yoon Y-G, Hoffmann M, Pak N, Wetzstein G, Kato S, Schrödel T, Raskar R, Zimmer M, Boyden ES und Vaziri A. Simultaneous whole-animal 3D-imaging of neuronal activity using light-field microscopy. Nature Methods Advance Online Publication, 18 March, 2014. DOI 10.1038/nmeth.2964.

Images and videos to illustrate this press release are available from the IMP Website at

About the IMP
The Research Institute of Molecular Pathology (IMP) in Vienna is a basic biomedical research institute largely sponsored by Boehringer Ingelheim. With over 200 scientists from 37 nations, the IMP is committed to scientific discovery of fundamental molecular and cellular mechanisms underlying complex biological phenomena. Research areas include cell and molecular biology, neurobiology, disease mechanisms and computational biology.

About the MFPL
The Max F. Perutz Laboratories (MFPL) are a center established by the
University of Vienna and the Medical University of Vienna to provide an
environment for excellent, internationally recognized research and
education in the field of Molecular Biology. Currently, the MFPL host around 60
independent research groups, involving more than 500 people from 40 nations.

Scientific Contact
Ass. Prof. Dr. Alipasha Vaziri, Gruppenleiter
Research Institute of Molecular Pathology (IMP)
Max F. Perutz Laboratories (MFPL) und Research Platform Quantum Phenomena & Nanoscale Biological Systems (QuNaBioS), University of Vienna
T +43-1-79730-3540

Media Contact
Dr. Heidemarie Hurtl, Communications
IMP Research Institute of Molecular Pathology
T +43-1-79730 3625

Weitere Informationen:

Dr. Heidemarie Hurtl | idw - Informationsdienst Wissenschaft

Further reports about: 3-D IMP MFPL Molecular Pathology QuNaBioS Quantum activity elegans neurons

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