For the first time, researchers have analyzed the multitude of microorganisms residing in the human gut as a complex, integrated biological system, rather than a set of separate species. Their approach has revealed patterns that correspond with excess body weight.
The collection of microbes inside the human gut is a bustling network of genetic interplays and energy use. By constructing models of these microbial communities, scientists have discovered novel differences between obese and lean people.
They were able to detect organizational shifts away from a normal "lean" state in the gut flora of people who were significantly overweight, as well as in people with inflammatory bowel disease. The findings are reported in the Proceedings of the National Academy of Sciences.
The senior author of the paper, Elhanan Borenstein, assistant professor of genome sciences at the University of Washington, said, "Our research introduces a novel framework, applying systems biology and in-silico (computer) modeling to study the human microbiome – the complex ensemble of microorganisms that populate the human body – as a single cohesive system."Sharon Greenblum of the UW Department of Genome Sciences and Peter J. Turnbaugh of the FAS Center for Systems Biology at Harvard University were on the research team. Borenstein also holds an adjunct appointment at the UW's Department of Computer Science & Engineering and is an External Professor at the Santa Fe Institute.
World-wide research initiatives, Borenstein said, highlight how the microbiome influences human health. The microbiome is essential for human development, immunity and nutrition.
People harbor more than 100 trillion microbes. These microbes live in various habitats on and within the human anatomy; the gut houses the densest population of all, containing hundreds of bacterial species. Their collective gene set is enormous: 150 times larger than the set of human genes.
The gut microbiome helps keep us alive by manufacturing vitamins and the building blocks of proteins, extracting energy from food, and conferring disease resistance. Previous research on the gut microbiome, including the transfer of a donor microbiome to a recipient, suggests that manipulation of the gut flora may have useful clinical applications.
"Characterizing the gut microbiome and its interactions with its human host has the potential to provide deep insight into normal human physiology and disease states," said Greenblum, the first author on the paper.
Researchers mapping the human microbiome are discovering previously uncharted species and genes. Genetic studies determining the relative abundance of different species in the human microbiome have linked various combinations of species to certain human conditions. Researchers have already observed that obese and lean people have differences in their gut microbiome.What preliminary findings are still missing, according to Borenstein, is a comprehensive, system-level understanding of how these variations in the genetic makeup of the microbiome affect its organization and consequently its metabolic potential (energy production, use and storage) and its effects on the human host.
The team used advanced computational techniques to reconstruct models of these microbial communities and the interactions between the various genes. The group also estimated the change in abundance of enzymes associated with the various host states: lean, obese, or affected with inflammatory bowel disease.
Their models reflected metabolic interdependencies between enzymes, not their physical location in the gut. Certain interactions were central to the microbial community's metabolism. However, those enzymes that typified obesity or leanness were mostly remote from the core network and its key metabolic functions. These enzymes worked in the periphery of the modeled network. These peripheral enzymes may represent metabolic first steps relying on substances not manufactured by the microbiome or end points releasing products not used by the microbiome, the researchers surmised.
Such enzymes, Borenstein explained, are likely to directly use or produce substances that characterize the gut environment, and form an interface between microbial and human metabolism.
"Our results suggest that the enzyme-level variation associated with obesity and inflammatory bowel disease relates to changes in how the microbiome interacts with the human gut environment, rather than a variation in the microbiome's core metabolic processes," Borenstein said.
He said other findings point to the obese microbiomes' potential ability to use diverse energy sources, which may account for their increased capacity to extract energy from the diet. This system approach also suggests possible biomarkers for obesity and inflammatory bowel disease. The markers may indicate common underlying triggers of disease or a response of the gut microbiome to disease.
Comparisons between the obese and lean microbiome network models also showed that obese microbiomes are associated with lower levels of a topological trait called "modularity." The reduced modularity of obese microbiome communities resembles that of single species that inhabit more constant environments.
While the associations drawn from the study may not clearly implicate a specific mechanism for complex and poorly understood diseases such as obesity and inflammatory bowel disease, Borenstein noted that they demonstrate the promise of using a systems biology approach to study the human microbiome and its contribution to human health.
The research was funded by the National Human Genome Research Institute, the National Institutes of Health, and the Alfred P. Sloan Foundation.
Leila Gray | EurekAlert!
A Map of the Cell’s Power Station
18.08.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau
On the way to developing a new active ingredient against chronic infections
21.08.2017 | Deutsches Zentrum für Infektionsforschung
Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.
As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...
Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.
Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...
For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.
While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...
An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.
The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...
A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.
Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...
16.08.2017 | Event News
04.08.2017 | Event News
26.07.2017 | Event News
21.08.2017 | Materials Sciences
21.08.2017 | Health and Medicine
21.08.2017 | Materials Sciences