Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New guideline for DNA sequences could prevent erroneous data

05.11.2012
DNA sequence data is an indispensable source of research information in biology. But not all data are reliable.
Almost 10% of all fungal DNA sequences are, for example, incorrectly identified to species level. A international team of researchers, with it’s core at the University of Gothenburg, Sweden, has therefore prepared a guide to assist the scientific community in the quality control process.

A new scientific study sees the researchers putting together a number of guidelines to help other researchers to ensure a high level of quality among their newly generated DNA sequences.

Detailed studies
DNA sequences make it possible to study biological samples and environments at a level of detail that traditional tools, such as microscopes, cannot provide. It is, for example, possible to investigate what species are present in seemingly barren substrates such as soil and seawater. Such studies often reveal an astonishing and hitherto unimagined diversity, and biology has made major advances as the use of DNA-based methods has become more widespread.

Quality varies
But as with many other sources of information, DNA sequences vary in quality and reliability. Several studies have found considerable quality problems in existing DNA sequence databases.

To verify ones DNA Sequence dataset for basic quality and authenticity has thus become an important part of biological research.

“Many researchers perceive quality control as difficult,” says Henrik Nilsson at the University of Gothenburg. “There are, quite simply, no guidelines that you can hand out to new or established researchers so that everyone is using the same approach. Which is why there are major differences in how, and to what extent, quality control is carried out in the research community.”

Nilsson is the lead author of a new scientific article on DNA sequence quality which has been published in the open-access journal MycoKeys.

Cumbersome software
One complication is that the software that is available to carry out parts of the quality control is cumbersome and often requires considerable computer capacity. The research group feels that it is not appropriate to require all biologists to have access to and be able to use such complex computer systems.

This is why they have written an article describing how quality control can be carried out manually without any tools beyond an Internet browser.

A guide that will help many
The article features a number of principles and observations on DNA sequences at different quality stages. Although the guidelines focus on fungi, where DNA sequences have had a particularly significant impact as a research instrument, they are general and can be used for most genes and groups of organisms.

The guidelines relate to traditional DNA sequencing as it is used in systematics, taxonomy and ecology.

The researchers hope that it will help readers to improve their DNA sequences and so halt the trend of increasing noise in the public DNA sequence databases.

Contact:
Henrik Nilsson, researcher, Department of Biological and Environmental Sciences
Tel: +46 (0)31 786 2623, e-mail: henrik.nilsson@bioenv.gu.se

Carina Eliasson | idw
Further information:
http://www.gu.se
http://dx.doi.org/10.3897/mycokeys.4.3606

More articles from Life Sciences:

nachricht Scientists unlock ability to generate new sensory hair cells
22.02.2017 | Brigham and Women's Hospital

nachricht New insights into the information processing of motor neurons
22.02.2017 | Max Planck Florida Institute for Neuroscience

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Breakthrough with a chain of gold atoms

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

Im Focus: DNA repair: a new letter in the cell alphabet

Results reveal how discoveries may be hidden in scientific “blind spots”

Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...

Im Focus: Dresdner scientists print tomorrow’s world

The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.

The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...

Im Focus: Mimicking nature's cellular architectures via 3-D printing

Research offers new level of control over the structure of 3-D printed materials

Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...

Im Focus: Three Magnetic States for Each Hole

Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".

Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Booth and panel discussion – The Lindau Nobel Laureate Meetings at the AAAS 2017 Annual Meeting

13.02.2017 | Event News

Complex Loading versus Hidden Reserves

10.02.2017 | Event News

International Conference on Crystal Growth in Freiburg

09.02.2017 | Event News

 
Latest News

Microhotplates for a smart gas sensor

22.02.2017 | Power and Electrical Engineering

Scientists unlock ability to generate new sensory hair cells

22.02.2017 | Life Sciences

Prediction: More gas-giants will be found orbiting Sun-like stars

22.02.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>