Thrombin plays a key role in various pathologies of the haemostatic system. Overexpression of thrombin can result in thrombosis, whereas its underexpression might lead to haemophilia.
Therefore, accurate monitoring of thrombin activity is crucial for determining the proper treatment of a given patient, as this correlates with the ability of blood to coagulate. A tool for monitoring the activity of thrombin over time is the so-called thrombin generation test (TGT).
In a collaborative project headed by Floris Rutjes (Radboud University Nijmegen, Netherlands), novel thrombin-specific fluorogenic peptides were developed for accurately determining thrombin concentrations by the TGT. Adding such a peptide substrate to a clotting plasma sample results in its hydrolysis by thrombin, thereby releasing the fluorophore.
Spectrophotometric measurement of fluorophore release increases the sensitivity and thus accuracy of the TGT, yielding various essential coagulation parameters. The use of fluorogenic peptides in combination with the TGT is expected to find broad application in the field of haemostasis and thrombosis.About the Author
Author: Floris P. J. T. Rutjes, Radboud Universiteit Nijmegen (The Netherlands), http://www.soc.science.ru.nl/
Floris P. J. T. Rutjes | Wiley-VCH
Not of Divided Mind
19.01.2017 | Hertie-Institut für klinische Hirnforschung (HIH)
CRISPR meets single-cell sequencing in new screening method
19.01.2017 | CeMM Forschungszentrum für Molekulare Medizin der Österreichischen Akademie der Wissenschaften
An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...
Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...
Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.
While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...
Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales
Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...
Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.
As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...
19.01.2017 | Event News
10.01.2017 | Event News
09.01.2017 | Event News
19.01.2017 | Earth Sciences
19.01.2017 | Life Sciences
19.01.2017 | Physics and Astronomy