Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Genome researchers at Bielefeld University decode the hamster genome

22.08.2013
Scientists present their findings in ‘Nature Biotechnology‘

Genome researchers from Bielefeld University’s Center for Biotechnology (CeBiTec) headed by Professor Dr. Alfred Pühler have succeeded in sequencing the genome of the Chinese hamster.


The genome sequencing started with the Chinese hamster (picture). Photo: Bielefeld University. Photo: Kerstin Molthagen

The Chinese hamster supplies the cell cultures used by the pharmaceutical industry to produce biopharmaceutical products such as antibodies used in medicine. This costly project was only possible thanks to a cooperation between Bielefeld University and its international project partners. The researchers have now published their results in the internationally renowned scientific journal ‘Nature Biotechnology‘.

To carry out this project, the CeBiTec research team cooperated with the University of Natural Resources and Life Sciences in Vienna (where the project was headed by Professor Dr. Nicole Borth), the Austrian Centre of Industrial Biotechnology (acib), and two pharmaceutical companies: Novartis (in Switzerland) and Pfizer (in the USA).

Professor Dr. Thomas Noll, Scientific Director of CeBiTec, is confident that the data they have obtained will be of great interest to science and industry. ‘In future, the decoded hamster genome will greatly advance the use of cell lines to produce pharmaceuticals’, says Noll, who runs the Cell Culture Technology research group at the Faculty of Technology and participated in the research project.

The genome of the Chinese hamster is composed of eleven pairs of chromosomes. Decoding such a large genome calls for the generation of large datasets that then have to be processed with bioinformatics. To facilitate the resulting data analysis, the researchers in Bielefeld and their colleagues in this project applied a completely new procedure that sorts the single chromosomes of the genome. The sequencing of the hamster chromosomes was performed by Dr. Karina Brinkrolf at CeBiTec. More than 1.4 billion short DNA sequences were generated with the help of modern instruments for next-generation sequencing. ‘The major challenge in this project was subsequently piecing these short DNA sequences together to form single total sequences of chromosomes’, explains the head of the project Professor Alfred Pühler. This work can only be done with powerful computers. ‘We had to complete the new CeBiTec computer cluster and apply new software before we could determine the genome sequence’, says the bioinformatics expert Dr. Alexander Goesmann who also worked on the project. ‘By decoding the hamster genome sequence’, notes Goesmann, ‘bioinformatics at Bielefeld University has broken new ground.’ With approximately 2.3 billion bases, the magnitude of the genome sequence of the Chinese hamster is comparable to that of the human genome.

The head of the project Alfred Pühler views this research as a milestone in the work at CeBiTec: ‘The decoding of the hamster genome successfully concludes a major CeBiTec project. The hamster sequence is available in the public domain and can be used for research throughout the world.’ The project greatly enhances the status of Bielefeld as a basis for current research on the cell cultures of the Chinese hamster, says Pühler. A further project has already been agreed with the University of Natural Resources and Life Sciences in Vienna and the Austrian Center of Industrial Biotechnology. ‘This places Bielefeld University in a good position to carry on contributing to this highly competitive field of research.’

Original publication:
Karina Brinkrolf, Oliver Rupp, Holger Laux, Florian Kollin, Wolfgang Ernst, Burkhard Linke, Rudolf Kofler, Sandrine Romand, Friedemann Hesse, Wolfgang E. Budach, Sybille Galosy, Dethardt Müller, Thomas Noll, Johannes Wienberg, Thomas Jostock, Mark Leonard, Johannes Grillari, Andreas Tauch, Alexander Goesmann, Bernhard Helk, John E. Mott, Alfred Pühler, and Nicole Borth: Chinese hamster genome sequenced from sorted chromosomes, Nature Biotechnology, http://dx.doi.org/10.1038/nbt.2645, published online on 8 August 2013
For further information in the Internet, go to:
www.cebitec.uni-bielefeld.de

Dr. Alfred Pühler | EurekAlert!
Further information:
http://www.cebitec.uni-bielefeld.de
http://www.uni-bielefeld.de

More articles from Life Sciences:

nachricht Multi-institutional collaboration uncovers how molecular machines assemble
02.12.2016 | Salk Institute

nachricht Fertilized egg cells trigger and monitor loss of sperm’s epigenetic memory
02.12.2016 | IMBA - Institut für Molekulare Biotechnologie der Österreichischen Akademie der Wissenschaften GmbH

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Novel silicon etching technique crafts 3-D gradient refractive index micro-optics

A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.

Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...

Im Focus: Quantum Particles Form Droplets

In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.

“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...

Im Focus: MADMAX: Max Planck Institute for Physics takes up axion research

The Max Planck Institute for Physics (MPP) is opening up a new research field. A workshop from November 21 - 22, 2016 will mark the start of activities for an innovative axion experiment. Axions are still only purely hypothetical particles. Their detection could solve two fundamental problems in particle physics: What dark matter consists of and why it has not yet been possible to directly observe a CP violation for the strong interaction.

The “MADMAX” project is the MPP’s commitment to axion research. Axions are so far only a theoretical prediction and are difficult to detect: on the one hand,...

Im Focus: Molecules change shape when wet

Broadband rotational spectroscopy unravels structural reshaping of isolated molecules in the gas phase to accommodate water

In two recent publications in the Journal of Chemical Physics and in the Journal of Physical Chemistry Letters, researchers around Melanie Schnell from the Max...

Im Focus: Fraunhofer ISE Develops Highly Compact, High Frequency DC/DC Converter for Aviation

The efficiency of power electronic systems is not solely dependent on electrical efficiency but also on weight, for example, in mobile systems. When the weight of relevant components and devices in airplanes, for instance, is reduced, fuel savings can be achieved and correspondingly greenhouse gas emissions decreased. New materials and components based on gallium nitride (GaN) can help to reduce weight and increase the efficiency. With these new materials, power electronic switches can be operated at higher switching frequency, resulting in higher power density and lower material costs.

Researchers at the Fraunhofer Institute for Solar Energy Systems ISE together with partners have investigated how these materials can be used to make power...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ICTM Conference 2017: Production technology for turbomachine manufacturing of the future

16.11.2016 | Event News

Innovation Day Laser Technology – Laser Additive Manufacturing

01.11.2016 | Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

 
Latest News

UTSA study describes new minimally invasive device to treat cancer and other illnesses

02.12.2016 | Medical Engineering

Plasma-zapping process could yield trans fat-free soybean oil product

02.12.2016 | Agricultural and Forestry Science

What do Netflix, Google and planetary systems have in common?

02.12.2016 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>