Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Genome researchers at Bielefeld University decode the hamster genome

22.08.2013
Scientists present their findings in ‘Nature Biotechnology‘

Genome researchers from Bielefeld University’s Center for Biotechnology (CeBiTec) headed by Professor Dr. Alfred Pühler have succeeded in sequencing the genome of the Chinese hamster.


The genome sequencing started with the Chinese hamster (picture). Photo: Bielefeld University. Photo: Kerstin Molthagen

The Chinese hamster supplies the cell cultures used by the pharmaceutical industry to produce biopharmaceutical products such as antibodies used in medicine. This costly project was only possible thanks to a cooperation between Bielefeld University and its international project partners. The researchers have now published their results in the internationally renowned scientific journal ‘Nature Biotechnology‘.

To carry out this project, the CeBiTec research team cooperated with the University of Natural Resources and Life Sciences in Vienna (where the project was headed by Professor Dr. Nicole Borth), the Austrian Centre of Industrial Biotechnology (acib), and two pharmaceutical companies: Novartis (in Switzerland) and Pfizer (in the USA).

Professor Dr. Thomas Noll, Scientific Director of CeBiTec, is confident that the data they have obtained will be of great interest to science and industry. ‘In future, the decoded hamster genome will greatly advance the use of cell lines to produce pharmaceuticals’, says Noll, who runs the Cell Culture Technology research group at the Faculty of Technology and participated in the research project.

The genome of the Chinese hamster is composed of eleven pairs of chromosomes. Decoding such a large genome calls for the generation of large datasets that then have to be processed with bioinformatics. To facilitate the resulting data analysis, the researchers in Bielefeld and their colleagues in this project applied a completely new procedure that sorts the single chromosomes of the genome. The sequencing of the hamster chromosomes was performed by Dr. Karina Brinkrolf at CeBiTec. More than 1.4 billion short DNA sequences were generated with the help of modern instruments for next-generation sequencing. ‘The major challenge in this project was subsequently piecing these short DNA sequences together to form single total sequences of chromosomes’, explains the head of the project Professor Alfred Pühler. This work can only be done with powerful computers. ‘We had to complete the new CeBiTec computer cluster and apply new software before we could determine the genome sequence’, says the bioinformatics expert Dr. Alexander Goesmann who also worked on the project. ‘By decoding the hamster genome sequence’, notes Goesmann, ‘bioinformatics at Bielefeld University has broken new ground.’ With approximately 2.3 billion bases, the magnitude of the genome sequence of the Chinese hamster is comparable to that of the human genome.

The head of the project Alfred Pühler views this research as a milestone in the work at CeBiTec: ‘The decoding of the hamster genome successfully concludes a major CeBiTec project. The hamster sequence is available in the public domain and can be used for research throughout the world.’ The project greatly enhances the status of Bielefeld as a basis for current research on the cell cultures of the Chinese hamster, says Pühler. A further project has already been agreed with the University of Natural Resources and Life Sciences in Vienna and the Austrian Center of Industrial Biotechnology. ‘This places Bielefeld University in a good position to carry on contributing to this highly competitive field of research.’

Original publication:
Karina Brinkrolf, Oliver Rupp, Holger Laux, Florian Kollin, Wolfgang Ernst, Burkhard Linke, Rudolf Kofler, Sandrine Romand, Friedemann Hesse, Wolfgang E. Budach, Sybille Galosy, Dethardt Müller, Thomas Noll, Johannes Wienberg, Thomas Jostock, Mark Leonard, Johannes Grillari, Andreas Tauch, Alexander Goesmann, Bernhard Helk, John E. Mott, Alfred Pühler, and Nicole Borth: Chinese hamster genome sequenced from sorted chromosomes, Nature Biotechnology, http://dx.doi.org/10.1038/nbt.2645, published online on 8 August 2013
For further information in the Internet, go to:
www.cebitec.uni-bielefeld.de

Dr. Alfred Pühler | EurekAlert!
Further information:
http://www.cebitec.uni-bielefeld.de
http://www.uni-bielefeld.de

More articles from Life Sciences:

nachricht Newly designed molecule binds nitrogen
23.02.2018 | Julius-Maximilians-Universität Würzburg

nachricht Atomic Design by Water
23.02.2018 | Max-Planck-Institut für Eisenforschung GmbH

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Attoseconds break into atomic interior

A newly developed laser technology has enabled physicists in the Laboratory for Attosecond Physics (jointly run by LMU Munich and the Max Planck Institute of Quantum Optics) to generate attosecond bursts of high-energy photons of unprecedented intensity. This has made it possible to observe the interaction of multiple photons in a single such pulse with electrons in the inner orbital shell of an atom.

In order to observe the ultrafast electron motion in the inner shells of atoms with short light pulses, the pulses must not only be ultrashort, but very...

Im Focus: Good vibrations feel the force

A group of researchers led by Andrea Cavalleri at the Max Planck Institute for Structure and Dynamics of Matter (MPSD) in Hamburg has demonstrated a new method enabling precise measurements of the interatomic forces that hold crystalline solids together. The paper Probing the Interatomic Potential of Solids by Strong-Field Nonlinear Phononics, published online in Nature, explains how a terahertz-frequency laser pulse can drive very large deformations of the crystal.

By measuring the highly unusual atomic trajectories under extreme electromagnetic transients, the MPSD group could reconstruct how rigid the atomic bonds are...

Im Focus: Developing reliable quantum computers

International research team makes important step on the path to solving certification problems

Quantum computers may one day solve algorithmic problems which even the biggest supercomputers today can’t manage. But how do you test a quantum computer to...

Im Focus: In best circles: First integrated circuit from self-assembled polymer

For the first time, a team of researchers at the Max-Planck Institute (MPI) for Polymer Research in Mainz, Germany, has succeeded in making an integrated circuit (IC) from just a monolayer of a semiconducting polymer via a bottom-up, self-assembly approach.

In the self-assembly process, the semiconducting polymer arranges itself into an ordered monolayer in a transistor. The transistors are binary switches used...

Im Focus: Demonstration of a single molecule piezoelectric effect

Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale

Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

2nd International Conference on High Temperature Shape Memory Alloys (HTSMAs)

15.02.2018 | Event News

Aachen DC Grid Summit 2018

13.02.2018 | Event News

How Global Climate Policy Can Learn from the Energy Transition

12.02.2018 | Event News

 
Latest News

Basque researchers turn light upside down

23.02.2018 | Physics and Astronomy

Finnish research group discovers a new immune system regulator

23.02.2018 | Health and Medicine

Attoseconds break into atomic interior

23.02.2018 | Physics and Astronomy

VideoLinks
Science & Research
Overview of more VideoLinks >>>