Genome researchers from Bielefeld University’s Center for Biotechnology (CeBiTec) headed by Professor Dr. Alfred Pühler have succeeded in sequencing the genome of the Chinese hamster.
The genome sequencing started with the Chinese hamster (picture). Photo: Bielefeld University. Photo: Kerstin Molthagen
The Chinese hamster supplies the cell cultures used by the pharmaceutical industry to produce biopharmaceutical products such as antibodies used in medicine. This costly project was only possible thanks to a cooperation between Bielefeld University and its international project partners. The researchers have now published their results in the internationally renowned scientific journal ‘Nature Biotechnology‘.
To carry out this project, the CeBiTec research team cooperated with the University of Natural Resources and Life Sciences in Vienna (where the project was headed by Professor Dr. Nicole Borth), the Austrian Centre of Industrial Biotechnology (acib), and two pharmaceutical companies: Novartis (in Switzerland) and Pfizer (in the USA).
Professor Dr. Thomas Noll, Scientific Director of CeBiTec, is confident that the data they have obtained will be of great interest to science and industry. ‘In future, the decoded hamster genome will greatly advance the use of cell lines to produce pharmaceuticals’, says Noll, who runs the Cell Culture Technology research group at the Faculty of Technology and participated in the research project.
The genome of the Chinese hamster is composed of eleven pairs of chromosomes. Decoding such a large genome calls for the generation of large datasets that then have to be processed with bioinformatics. To facilitate the resulting data analysis, the researchers in Bielefeld and their colleagues in this project applied a completely new procedure that sorts the single chromosomes of the genome. The sequencing of the hamster chromosomes was performed by Dr. Karina Brinkrolf at CeBiTec. More than 1.4 billion short DNA sequences were generated with the help of modern instruments for next-generation sequencing. ‘The major challenge in this project was subsequently piecing these short DNA sequences together to form single total sequences of chromosomes’, explains the head of the project Professor Alfred Pühler. This work can only be done with powerful computers. ‘We had to complete the new CeBiTec computer cluster and apply new software before we could determine the genome sequence’, says the bioinformatics expert Dr. Alexander Goesmann who also worked on the project. ‘By decoding the hamster genome sequence’, notes Goesmann, ‘bioinformatics at Bielefeld University has broken new ground.’ With approximately 2.3 billion bases, the magnitude of the genome sequence of the Chinese hamster is comparable to that of the human genome.
The head of the project Alfred Pühler views this research as a milestone in the work at CeBiTec: ‘The decoding of the hamster genome successfully concludes a major CeBiTec project. The hamster sequence is available in the public domain and can be used for research throughout the world.’ The project greatly enhances the status of Bielefeld as a basis for current research on the cell cultures of the Chinese hamster, says Pühler. A further project has already been agreed with the University of Natural Resources and Life Sciences in Vienna and the Austrian Center of Industrial Biotechnology. ‘This places Bielefeld University in a good position to carry on contributing to this highly competitive field of research.’
Researchers develop eco-friendly, 4-in-1 catalyst
25.04.2017 | Brown University
Transfecting cells gently – the LZH presents a GNOME prototype at the Labvolution 2017
25.04.2017 | Laser Zentrum Hannover e.V.
More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.
Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...
Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.
"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...
The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.
Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...
The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...
Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.
Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...
20.04.2017 | Event News
18.04.2017 | Event News
03.04.2017 | Event News
25.04.2017 | Physics and Astronomy
25.04.2017 | Materials Sciences
25.04.2017 | Life Sciences