The strategy promises to clarify the longstanding mystery of the role played by vast stretches of DNA sequence that do not code for the functional units—genes—that nevertheless may have a powerful regulatory influence. The research is described in the 12 February edition of the journal Nature.
"Our approach employs next generation sequencing technology to find regulatory regions, the 'switches' on a genome-wide scale and much more cost effectively," said Pennacchio. "It's the next layer of knowledge that's been missing."
The DOE JGI was founded in 1997 to accelerate the completion of the HGP and completed the DOE's commitment to sequence three (5, 16, 19) of the 23 chromosomes, totaling 11 percent of the human genome, and published the analysis in Nature back in 2004.
In this newly published study, Pennacchio, lead authors DOE scientist Axel Visel and postdoctoral fellow Matthew Blow, and their colleagues, describe a shortcut for identifying gene regulatory regions or the molecular switches that turn on or off gene expression.
Using what's called ChIP-Sequencing or ChIP-Seq, chromatin immunoprecipitation (ChIP) is combined with massively parallel DNA sequencing to identify binding sites of DNA-associated proteins.
Traditionally researchers have relied on evolution to guide them to non-coding sequences that are likely to have a function—such as enhancing the expression of genes. Via the public genome databases, they would align the entire human genome code with that of other vertebrate species (e.g. other mammals, birds, frogs, fish) and then look for sequences that are conserved in evolution.
"Most protein-coding sequences show signs of conservation between species, but there is also a large number of non-coding sequences that have been surprisingly well conserved for tens or even hundreds of millions of years," said Visel. "This suggests that these regions, formerly thought to be "junk" DNA, actually have some functional relevance and are under selection because sequence changes reduce fitness of affected individuals. Using such sequence conservation, we have in previous studies identified enhancer candidate regions and shown in transgenic mouse experiments that these conserved non-coding regions are in fact often enhancers that are active during embryonic development. Conservation-based methods are relatively good at finding enhancers in the genome, but an important limitation is that they don't tell us where and when that particular enhancer would be active and thereby drive the expression of its neighboring target gene(s).
The older methods lacked specificity, Blow said. "For example, if we have a gene that is important both for brain and for limb development, we would not have been able to specifically identify the enhancer sequences near that gene that would drive the expression in the brain or limb, the only way to find out was to test these activities in experiments one-by-one, which is slow and can't be done on a genomic scale.
"Using this new method, we can directly identify a genome-wide set of enhancers that are active in a particular anatomical region or tissue at a particular time-point, which is an important advantage over conservation-based methods because in addition to telling us where an enhancer is located in the genome, it also provides an initial experimental characterization where we should expect this enhancer to be active."
The team used ChIP linked with a particular enhancer-associated protein, p300, then directed DOE JGI's massively parallel next generation sequencing capacity to map several thousand sites in mouse embryonic forebrain, midbrain and limb tissue. Over 80 of these fragments were tested in transgenic mouse experiments indicating an almost perfect success rate of p300-ChIP-Seq for identifying enhancers active in vivo.
"Enhancers are especially important for regulating genes during embryonic development," said Pennacchio. "They can regulate genes over long distances and switch on their target genes during very specific time-points and in very specific anatomical structures during development. There are several examples of mutations in such enhancers that cause disease in humans because genes are not expressed at the right time or in the right place anymore. A fundamental problem in studying such enhancers is that until recently we did not have effective tools to even find them in the genome on a large scale.
Pennacchio said that this new method will prove useful to the greater genomics and biomedical community for characterizing the role of the vast non-coding regions—dubbed genome "dark matter"—about which little is known.
"These datasets will also help to identify mutations in enhancers that play a role in human disease," Pennacchio said. "Human genetic studies indicate that in many cases disease is caused by mutations in non-coding sequences, but it has been difficult to study this in detail because the function of most non-coding sequences is poorly understood. Eventually, this will be useful for purposes including disease detection and personalized medicine."
With the rapidly increasing efficiency and cost-savings of the next generation sequencing technologies, a deluge of data from individual human genomes are being to come to light, to the point where whole-genome sequencing of patients may soon become a standard diagnostic tool.
"While progress is being made towards this goal, it is important to keep in mind that our current understanding of the genome has focused on protein-coding sequences," said Pennacchio. "Datasets like the one provided through this study will be important to understand the remaining 98 percent of the genome and what its role in health and disease is."
The published study provides an important proof of principle to establish and validate a new method in three different mouse tissues at a single embryonic time-point, Pennacchio said. "We can now generate genome-wide enhancer datasets directly from human tissues and compare genome-wide sets of enhancer activities between healthy people and people suffering from disease, which may reveal how enhancer activities change on a global scale in these disease states."
David Gilbert | EurekAlert!
Further reports about: > ChIP-Seq > ChIP-Sequencing > DNA > DNA sequence > DNA-associated proteins > Genom > Nature Immunology > biofuels production > chromatin immunoprecipitation > embryonic development > human genome > molecular tools > multicellular organisms > non-coding sequence > on-off switches in genomes > protein-coding genes
The birth of a new protein
20.10.2017 | University of Arizona
Building New Moss Factories
20.10.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau
University of Maryland researchers contribute to historic detection of gravitational waves and light created by event
On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...
Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.
Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....
Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).
When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...
Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.
How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...
Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.
It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...
17.10.2017 | Event News
10.10.2017 | Event News
10.10.2017 | Event News
20.10.2017 | Information Technology
20.10.2017 | Materials Sciences
20.10.2017 | Interdisciplinary Research