Gap closed in the genetic map of kingdom fungi

Today, the genomes of more than 250 fungi have been sequenced. Among the basal filamentous ascomycetes – a group of ascomycetes that includes e.g. truffles and morels – only one representative has been analysed so far: the truffle Tuber melanosporum.

“With 125 million base pairs, the truffle genome is unusually big, yet it is coding for relatively few genes, namely some 7,500,” says Minou Nowrousian from the Department of General and Molecular Botany. “Until now, it was not clear whether this is typical of basal filamentous ascomycetes or whether it is caused by the truffle's 'atypical' lifestyle.”

Unlike other filamentous ascomycetes, the truffle does not develop reproductive organs – so-called fruiting bodies – above ground but rather below ground. Moreover, it only grows in symbiosis with plant roots (mycorrhiza). Pyronema, on the other hand, is a typical representative of its group.

Intermediary evolutionary stage

The genome of Pyronema confluens contains 50 million base pairs and some 13,000 genes; it is thus smaller than that of the truffle, and yet it contains more genes. These findings confirm the truffle's special position and provide new insights into the evolution of ascomycetes. “Pyronema confluens bears a stronger resemblance to higher ascomycetes than to the truffle,” concludes Minou Nowrousian. However, the scientists have also discovered differences to higher ascomycetes, for example in the DNA sequence containing the genetic blueprint for mating type genes. Mating type genes are the main regulators of sexual development and, in Pyronema confluens, they do not show the standardised structure that is typical for higher ascomycetes. “Pyronema confluens may represent an intermediary evolutionary stage in the evolution of mating type genes,” says the Bochum biologist.

Light-activated genes

One characteristic feature of the fungus under investigation is the fact that it produces fruiting bodies only in light. Fittingly, the researchers discovered genes in the Pyronema genome containing blueprints for photoreceptors for different wavelengths of visible light. The activity of some of those genes increased in light.

Pyronema – a typical representative of its systematic group

Project funding

The German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) funded the project headed by PD Dr Minou Nowrousian (NO 407/4-1).
Bibliographic record

Traeger S, Altegoer F, Freitag M, Gabaldon T, Kempken F, Kumar F, Marcet-Houben M, Pöggeler S, Stajich JE, Nowrousian M (2013) The genome and development-dependent transcriptomes of Pyronema confluens: a window into fungal evolution. PLoS Genet 9(9): e1003820. doi:10.1371/journal.pgen.1003820
Further information

PD Dr Minou Nowrousian
Department of General and Molecular Botany
Faculty of Biology and Biotechnology at the Ruhr-Universität
44780 Bochum, Germany
phone: +49/234/32-24588
e-mail: minou.nowrousian@rub.de