The researchers also determined that the type of facility where sperm is taken and its distance from the IVF laboratory has no bearing on pregnancy outcomes.
Pictured is a section of human testicular tissue and sperm cells under confocal microscopy.
The findings, published online in PLOS ONE, may benefit men with no sperm in their semen due to genetics, cancer diagnoses or testicular failure. These men do not have enough sperm to have their own children through IVF without being biopsied.
In these men, a biopsy in a testicle often results in enough sperm to be used in a procedure called intracytoplasmic sperm injection (ICSI) as part of the IVF process in men with severe infertility.
The biopsy, which is usually performed on an outpatient basis, involves obtaining tissue from testicles that is examined for sperm. If sperm are found, they are removed and used immediately in ICSI or frozen for future use.
In ICSI, a single sperm is injected into an egg that has been surgically retrieved from a woman’s ovaries. The fertilized egg, or embryo, then is transferred to the woman’s womb.
“The convenience and ease of being able to use frozen sperm taken by biopsy in ICSI offers many advantages over fresh sperm,” said Kenan Omurtag, MD, the study’s first author and assistant professor of obstetrics and gynecology.“
When fresh sperm is used, the biopsy for the sperm retrieval needs to be done the day before or on the same day as egg retrieval from the woman, and this may not be convenient for the couple. When frozen sperm is used, the man can have his biopsy first, and if sperm is found, it can be banked. Then, at the couple’s convenience, the woman’s eggs are retrieved and ICSI can be completed.
Before ICSI, a woman takes daily injections of fertility medications for a week to 10 days to stimulate her ovaries to produce eggs. Using an ultrasound to locate eggs, a doctor then removes them from the woman’s ovaries with a fine, hollow needle.
In this study, researchers analyzed data from 1995 through 2009 from the Washington University Infertility and Reproductive Medicine Center.
One hundred thirty-six men had testicular biopsies to be used in ICSI. Of those biopsies, 84 percent involved frozen sperm and the remaining 16 percent used fresh sperm. A statistically significant difference in fertilization rate was noted between frozen sperm (62 percent) and fresh (47 percent) sperm, respectively. There was no difference between the delivery rate.
Two urologists performed 150 testicular sperm biopsies in an operating room adjacent to the IVF lab, in an operating room in another building almost one mile from the IVF lab and in an ambulatory surgical center about 15 miles from the IVF lab. The maximum travel time from the third site was less than 30 minutes.
There was no statistically significant difference between the locations and pregnancy results.
“Men with no sperm in their semen now have more options to have children of their own,” said Randall Odem, MD, co-author of the study and professor of obstetrics and gynecology, who recommends that men with infertility issues seek treatment from a reproductive endocrinologist and a urologist trained in male infertility.
“This study demonstrates that using frozen sperm taken by biopsy works as well for most patients in what matters most —pregnancy rates,” he said.
Omurtag K, Cooper A, Bullock A, Naughton C, Ratts V, Odem R, Lanzendorf, SE. Sperm recovery and IVF after Testicular sperm extraction (TESE): Effect of male diagnosis and use of off-site surgical centers on sperm recovery and IVF. PLOS ONE vol. 8 (7), published online July 29, 2013.
Washington University School of Medicine’s 2,100 employed and volunteer faculty physicians also are the medical staff of Barnes-Jewish and St. Louis Children’s hospitals. The School of Medicine is one of the leading medical research, teaching and patient care institutions in the nation, currently ranked sixth in the nation by U.S. News & World Report. Through its affiliations with Barnes-Jewish and St. Louis Children’s hospitals, the School of Medicine is linked to BJC HealthCare.
Judy Martin | EurekAlert!
Seeking structure with metagenome sequences
20.01.2017 | DOE/Joint Genome Institute
Snap, Digest, Respire
20.01.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau
An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...
Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...
Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.
While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...
Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales
Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...
Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.
As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...
19.01.2017 | Event News
10.01.2017 | Event News
09.01.2017 | Event News
20.01.2017 | Life Sciences
20.01.2017 | Physics and Astronomy
20.01.2017 | Materials Sciences