The researchers also determined that the type of facility where sperm is taken and its distance from the IVF laboratory has no bearing on pregnancy outcomes.
Pictured is a section of human testicular tissue and sperm cells under confocal microscopy.
The findings, published online in PLOS ONE, may benefit men with no sperm in their semen due to genetics, cancer diagnoses or testicular failure. These men do not have enough sperm to have their own children through IVF without being biopsied.
In these men, a biopsy in a testicle often results in enough sperm to be used in a procedure called intracytoplasmic sperm injection (ICSI) as part of the IVF process in men with severe infertility.
The biopsy, which is usually performed on an outpatient basis, involves obtaining tissue from testicles that is examined for sperm. If sperm are found, they are removed and used immediately in ICSI or frozen for future use.
In ICSI, a single sperm is injected into an egg that has been surgically retrieved from a woman’s ovaries. The fertilized egg, or embryo, then is transferred to the woman’s womb.
“The convenience and ease of being able to use frozen sperm taken by biopsy in ICSI offers many advantages over fresh sperm,” said Kenan Omurtag, MD, the study’s first author and assistant professor of obstetrics and gynecology.“
When fresh sperm is used, the biopsy for the sperm retrieval needs to be done the day before or on the same day as egg retrieval from the woman, and this may not be convenient for the couple. When frozen sperm is used, the man can have his biopsy first, and if sperm is found, it can be banked. Then, at the couple’s convenience, the woman’s eggs are retrieved and ICSI can be completed.
Before ICSI, a woman takes daily injections of fertility medications for a week to 10 days to stimulate her ovaries to produce eggs. Using an ultrasound to locate eggs, a doctor then removes them from the woman’s ovaries with a fine, hollow needle.
In this study, researchers analyzed data from 1995 through 2009 from the Washington University Infertility and Reproductive Medicine Center.
One hundred thirty-six men had testicular biopsies to be used in ICSI. Of those biopsies, 84 percent involved frozen sperm and the remaining 16 percent used fresh sperm. A statistically significant difference in fertilization rate was noted between frozen sperm (62 percent) and fresh (47 percent) sperm, respectively. There was no difference between the delivery rate.
Two urologists performed 150 testicular sperm biopsies in an operating room adjacent to the IVF lab, in an operating room in another building almost one mile from the IVF lab and in an ambulatory surgical center about 15 miles from the IVF lab. The maximum travel time from the third site was less than 30 minutes.
There was no statistically significant difference between the locations and pregnancy results.
“Men with no sperm in their semen now have more options to have children of their own,” said Randall Odem, MD, co-author of the study and professor of obstetrics and gynecology, who recommends that men with infertility issues seek treatment from a reproductive endocrinologist and a urologist trained in male infertility.
“This study demonstrates that using frozen sperm taken by biopsy works as well for most patients in what matters most —pregnancy rates,” he said.
Omurtag K, Cooper A, Bullock A, Naughton C, Ratts V, Odem R, Lanzendorf, SE. Sperm recovery and IVF after Testicular sperm extraction (TESE): Effect of male diagnosis and use of off-site surgical centers on sperm recovery and IVF. PLOS ONE vol. 8 (7), published online July 29, 2013.
Washington University School of Medicine’s 2,100 employed and volunteer faculty physicians also are the medical staff of Barnes-Jewish and St. Louis Children’s hospitals. The School of Medicine is one of the leading medical research, teaching and patient care institutions in the nation, currently ranked sixth in the nation by U.S. News & World Report. Through its affiliations with Barnes-Jewish and St. Louis Children’s hospitals, the School of Medicine is linked to BJC HealthCare.
Judy Martin | EurekAlert!
Symbiotic bacteria: from hitchhiker to beetle bodyguard
28.04.2017 | Johannes Gutenberg-Universität Mainz
Nose2Brain – Better Therapy for Multiple Sclerosis
28.04.2017 | Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB
More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.
Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...
Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.
"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...
The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.
Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...
The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...
Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.
Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...
28.04.2017 | Event News
20.04.2017 | Event News
18.04.2017 | Event News
28.04.2017 | Medical Engineering
28.04.2017 | Earth Sciences
28.04.2017 | Life Sciences