The researchers also determined that the type of facility where sperm is taken and its distance from the IVF laboratory has no bearing on pregnancy outcomes.
Pictured is a section of human testicular tissue and sperm cells under confocal microscopy.
The findings, published online in PLOS ONE, may benefit men with no sperm in their semen due to genetics, cancer diagnoses or testicular failure. These men do not have enough sperm to have their own children through IVF without being biopsied.
In these men, a biopsy in a testicle often results in enough sperm to be used in a procedure called intracytoplasmic sperm injection (ICSI) as part of the IVF process in men with severe infertility.
The biopsy, which is usually performed on an outpatient basis, involves obtaining tissue from testicles that is examined for sperm. If sperm are found, they are removed and used immediately in ICSI or frozen for future use.
In ICSI, a single sperm is injected into an egg that has been surgically retrieved from a woman’s ovaries. The fertilized egg, or embryo, then is transferred to the woman’s womb.
“The convenience and ease of being able to use frozen sperm taken by biopsy in ICSI offers many advantages over fresh sperm,” said Kenan Omurtag, MD, the study’s first author and assistant professor of obstetrics and gynecology.“
When fresh sperm is used, the biopsy for the sperm retrieval needs to be done the day before or on the same day as egg retrieval from the woman, and this may not be convenient for the couple. When frozen sperm is used, the man can have his biopsy first, and if sperm is found, it can be banked. Then, at the couple’s convenience, the woman’s eggs are retrieved and ICSI can be completed.
Before ICSI, a woman takes daily injections of fertility medications for a week to 10 days to stimulate her ovaries to produce eggs. Using an ultrasound to locate eggs, a doctor then removes them from the woman’s ovaries with a fine, hollow needle.
In this study, researchers analyzed data from 1995 through 2009 from the Washington University Infertility and Reproductive Medicine Center.
One hundred thirty-six men had testicular biopsies to be used in ICSI. Of those biopsies, 84 percent involved frozen sperm and the remaining 16 percent used fresh sperm. A statistically significant difference in fertilization rate was noted between frozen sperm (62 percent) and fresh (47 percent) sperm, respectively. There was no difference between the delivery rate.
Two urologists performed 150 testicular sperm biopsies in an operating room adjacent to the IVF lab, in an operating room in another building almost one mile from the IVF lab and in an ambulatory surgical center about 15 miles from the IVF lab. The maximum travel time from the third site was less than 30 minutes.
There was no statistically significant difference between the locations and pregnancy results.
“Men with no sperm in their semen now have more options to have children of their own,” said Randall Odem, MD, co-author of the study and professor of obstetrics and gynecology, who recommends that men with infertility issues seek treatment from a reproductive endocrinologist and a urologist trained in male infertility.
“This study demonstrates that using frozen sperm taken by biopsy works as well for most patients in what matters most —pregnancy rates,” he said.
Omurtag K, Cooper A, Bullock A, Naughton C, Ratts V, Odem R, Lanzendorf, SE. Sperm recovery and IVF after Testicular sperm extraction (TESE): Effect of male diagnosis and use of off-site surgical centers on sperm recovery and IVF. PLOS ONE vol. 8 (7), published online July 29, 2013.
Washington University School of Medicine’s 2,100 employed and volunteer faculty physicians also are the medical staff of Barnes-Jewish and St. Louis Children’s hospitals. The School of Medicine is one of the leading medical research, teaching and patient care institutions in the nation, currently ranked sixth in the nation by U.S. News & World Report. Through its affiliations with Barnes-Jewish and St. Louis Children’s hospitals, the School of Medicine is linked to BJC HealthCare.
Judy Martin | EurekAlert!
Single-stranded DNA and RNA origami go live
15.12.2017 | Wyss Institute for Biologically Inspired Engineering at Harvard
New antbird species discovered in Peru by LSU ornithologists
15.12.2017 | Louisiana State University
DNA molecules that follow specific instructions could offer more precise molecular control of synthetic chemical systems, a discovery that opens the door for engineers to create molecular machines with new and complex behaviors.
Researchers have created chemical amplifiers and a chemical oscillator using a systematic method that has the potential to embed sophisticated circuit...
MPQ scientists achieve long storage times for photonic quantum bits which break the lower bound for direct teleportation in a global quantum network.
Concerning the development of quantum memories for the realization of global quantum networks, scientists of the Quantum Dynamics Division led by Professor...
Researchers have developed a water cloaking concept based on electromagnetic forces that could eliminate an object's wake, greatly reducing its drag while...
Tiny pores at a cell's entryway act as miniature bouncers, letting in some electrically charged atoms--ions--but blocking others. Operating as exquisitely sensitive filters, these "ion channels" play a critical role in biological functions such as muscle contraction and the firing of brain cells.
To rapidly transport the right ions through the cell membrane, the tiny channels rely on a complex interplay between the ions and surrounding molecules,...
The miniaturization of the current technology of storage media is hindered by fundamental limits of quantum mechanics. A new approach consists in using so-called spin-crossover molecules as the smallest possible storage unit. Similar to normal hard drives, these special molecules can save information via their magnetic state. A research team from Kiel University has now managed to successfully place a new class of spin-crossover molecules onto a surface and to improve the molecule’s storage capacity. The storage density of conventional hard drives could therefore theoretically be increased by more than one hundred fold. The study has been published in the scientific journal Nano Letters.
Over the past few years, the building blocks of storage media have gotten ever smaller. But further miniaturization of the current technology is hindered by...
11.12.2017 | Event News
08.12.2017 | Event News
07.12.2017 | Event News
15.12.2017 | Power and Electrical Engineering
15.12.2017 | Materials Sciences
15.12.2017 | Life Sciences