Three years ago Dr. Philipp G. Maass from the Experimental and Clinical Research Center (ECRC), a joint cooperation between the Charité and the Max Delbrück Center (MDC) in Berlin, has discovered an epigenetic mechanism, which, when dysregulated, causes this condition. Now, he and Dr. Sylvia Bähring were able to show how this regulator functions and influences the development of the bones and shed light on a new principle of gene regulation (JCI, doi: 10.1172/JCI65508)*.
The hand on the left X-ray is normal. The hand to the right shows shortened single bones in the fingers of a patient with brachydaktyly type E (see arrows). The shortened extremity is due to the translocation of the gene PTHLH from chromosome 12 to chromosome 4 followed by dysregulation of the gene PTHLH. The regulator CISTR-ACT with its long non-coding RNA (lncRNA) was disrupted by the translocation and supported the dysregulation.
(Graphics and X-rays: Philipp Maass/Copyright: ECRC)
The gene causing brachydactyly type E (BDE) is PTHLH (the abbreviation stands for parathyroid hormone like hormone), and belongs to a group of genes that regulate the development of cartilage and determine subsequent skeletal structure. The researchers investigated two families with BDE. The patients exhibit shortened metacarpals, involved in forming the hands and feet, but had no other clinical symptoms.
Up to now, more than ten different forms of brachydactyly are known. The features of the hands and feet are variable depending upon which type of brachydactyly a patient has. Sometimes, the brachydactyly can be associated with hypertension, mental retardation, or other medical problems.
First, they could show, that the gene regulator interacts with genes over very long distances on the same chromosome (cis) and that it also is able to regulate genes on other chromosomes (trans). Thus, the tongue-twister name for this regulator: cis and trans-chromosomal communicator acting through DNA and noncoding RNA (CISTR-ACT).
Second, the team showed that because of a balanced translocation, CISTR-ACT is misplaced, so that the regulator no longer can properly influence PTHLH function.
Third, CISTR-ACT encodes a so-called long noncoding RNA that participates in the regulatory functions. This finding encompasses a new principle in gene regulation. Epigenetics refers to inherited mechanisms that occur without alterations in the DNA gene sequence. In this form of BDE, no change in the DNA sequence of coding genes is responsible for the condition.
Back to the first finding, the epigenetic regulator CISTR-ACT on chromosome 12 manages to get in touch with the gene PTHLH over a distance of 24 million base pairs. “The largest ever measured distance between a gene regulator and a gene on the same chromosome was around one million base-pairs”, explains Dr. Maass. Furthermore, CISTR-ACT regulates another developmental gene (SOX9) on chromosome 17. “This finding is extraordinary,” comments Dr. Maass.
How is this regulation possible? The researchers found the solution at the chromatin level, in which the chromosomes are densely packed. “Just imagine a ball of wool in which different threads actually touch each other at special points. At one point you have the gene, the other point symbolizes the gene regulator. “It is through this physical contact that CISTR-ACT regulates certain genes such as PTHLH very precisely in a specific tissue,” Dr. Maass and Dr. Bähring explain. The researchers could thus show that huge chromosomal loops build up on chromosome 12. Moreover, the epigenetic regulator, CISTR-ACT on chromosome 12 is somehow able to get in touch with its target SOX9 on chromosome 17.Translocation on different chromosomes
Translocations also influence gene expression, that is the production of proteins which built up and maintain the body`s tissues. Dr. Maass and Dr. Bähring found out that in their patients with BDE these translocations separate the gene PTHLH from its regulator CISTR-ACT, which reduces the expression of the gene during the development of cartilage. This state-of-affairs results in the premature maturation of the cartilage cells during the development of the extremities, leading to single shortened bones in the hands or feet of the patients with BDE.New insights into the dogma of gene regulation
The researchers in Berlin also showed that CISTR-ACT not only functions as a DNA-regulator, but also encodes a long non-coding RNA (lncRNA). Recently, researchers have begun looking at these lncRNA, because they appear to play an important role in organ development. Contrary to protein-coding genes these lncRNA do not produce proteins but instead serve their function in an epigenetic fashion. lncRNAs are distinguished by their length (greater than 200 nucleotides). Often, lncRNAs are encoded in many exons spread over large, intergenic DNA regions. Various diverse functions have been proposed for lncRNAs, including roles in regulating DNA metabolism, chromatin structure, and gene expression.*A misplaced lncRNA causes brachydactyly in humans
5 Hospital for Special Surgery, Laboratory for Cartilage Biology, Weill Cornell Medical College, Caspary Research Building, 535 E. 70th Street, New York, NY 10021, USAContact:
Barbara Bachtler | Max-Delbrück-Centrum
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