Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Finding Cancer Cells in Blood

02.03.2012
Chip-based method for the rapid, sensitive isolation of rare cells in blood

Even in the early stages of cancer, individual cancer cells can be found in the blood. Certain subsets of these circulating tumor cells can cause metastasis.



In cases of breast cancer, it is known that these cells can differ from the original tumor cells, which allows them to survive treatment to cause later recurrence. It could thus be quite informative to detect these cells in the blood and examine them more closely. In the journal Angewandte Chemie, researchers at the University of Washington (Seattle, USA) describe a new chip-based method that allows for the detection and isolation of tiny concentrations of such cells in blood.

The detection of circulating tumor cells is a difficult challenge because it requires the detection of quantities as low as one to ten cells per milliliter of blood—in the presence of large numbers of red blood cells and other cells. Conventional methods cannot manage this, but scientists led by Daniel T. Chiu have now developed a microfluidic system that allows for the analysis of 1 mL of blood within 20 minutes. The secret of their success is to virtually divide the sample into aliquots (portions) and to search these for the presence or absence of the desired cell types.

The blood is initially marked with fluorescent markers that specifically bind to the desired tumor cells. The sample is then passed through a system of microchannels, where it passes through a zone that is irradiated by a laser. The size of this zone determines the volume of the virtual aliquot; 2 nanoliters was found to work well. The laser causes the marker to fluoresce if marked cells are present. It can thus be determined whether or not an aliquot contains one (or more) of the desired cells. If the aliquot fluoresces, it is automatically pumped into a different channel than the fractions that do not fluoresce. The positive aliquots enter a filtration chamber. Red blood cells and the majority of blood cells pass through the filter; tumor cells are larger and are trapped. They can be counted on the filter, examined by microscope, or removed by micropipette for further examination.

By using a second marker, certain subpopulations, such as tumor stem cells, can be identified.

Experiments with blood containing a known number of breast cancer cells yielded a recovery rate of 93 % and a false positive rate of zero. Real blood samples from patients were also examined and the results compared with those from a clinically established system. The new microfluidic system proved to be significantly more sensitive. This new procedure has potential for monitoring treatment, aftercare, and the early detection of cancer.

About the Author
Daniel T. Chiu is the A. Bruce Montgomery Professor of Chemistry and Bioengineering at the University of Washington in Seattle. He specializes in the development and translation of new biotechnologies to biomedical and clinical applications. He is also a member of the Fred Hutchinson Cancer Research Center's cancer consortium, and the University of Washington's Center for Nanotechnology, and Neurobiology and Behavior program.
Author: Daniel T. Chiu, University of Washington, Seattle (USA), http://depts.washington.edu/chem/people/faculty/chiu.html
Title: Sensitive and High-Throughput Isolation of Rare Cells from Peripheral Blood with Ensemble-Decision Aliquot Ranking

Angewandte Chemie International Edition, Permalink to the article: http://dx.doi.org/10.1002/anie.201108695

Daniel T. Chiu | Angewandte Chemie
Further information:
http://pressroom.angewandte.org.

More articles from Life Sciences:

nachricht What the world's tiniest 'monster truck' reveals
23.08.2017 | American Chemical Society

nachricht Treating arthritis with algae
23.08.2017 | Empa - Eidgenössische Materialprüfungs- und Forschungsanstalt

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Fizzy soda water could be key to clean manufacture of flat wonder material: Graphene

Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.

As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...

Im Focus: Exotic quantum states made from light: Physicists create optical “wells” for a super-photon

Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.

Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...

Im Focus: Circular RNA linked to brain function

For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.

While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...

Im Focus: RAVAN CubeSat measures Earth's outgoing energy

An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.

The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...

Im Focus: Scientists shine new light on the “other high temperature superconductor”

A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.

Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Call for Papers – ICNFT 2018, 5th International Conference on New Forming Technology

16.08.2017 | Event News

Sustainability is the business model of tomorrow

04.08.2017 | Event News

Clash of Realities 2017: Registration now open. International Conference at TH Köln

26.07.2017 | Event News

 
Latest News

What the world's tiniest 'monster truck' reveals

23.08.2017 | Life Sciences

Treating arthritis with algae

23.08.2017 | Life Sciences

Witnessing turbulent motion in the atmosphere of a distant star

23.08.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>