Now Paul Li and colleagues at Simon Fraser University in Burnaby, Canada have combined DNA microarrays with microfluidic devices, which are used for the precise control of liquids at the nanoscale. In an upcoming issue of the journal Biomicrofluidics, which is published by the American Institute of Physics (AIP), Li and his colleagues describe how the first combined device can be used for probing and detecting DNA.
The key to Li's result: gold nanoparticles. Suspended in liquid and mixed with DNA, the nanometer-scale spheres of gold act as mini magnets that adhere to each of the DNA's twin strands. When the DNA is heated, the two strands separate, and the gold nanoparticles keep them apart, which allows the single strands to be probed with other pieces of DNA that are engineered to recognize particular sequences.
Li, whose work is funded by the Natural Sciences and Engineering Research Council of Canada, is applying for a patent for his technique. He sees a host of benefits from the combination of DNA microarrays and microfluidics.
"It's faster and requires a relatively small sample," he says, adding in his paper that "the whole procedure is accomplished at room temperature in an hour and apparatus for high temperature… is not required"
The article, "Gold nanoparticle-assisted single base-pair mismatch discrimination on a microfluidic microarray device" by Lin Wang and Paul C. Li will appear in the journal Biomicrofluidics. See: http://bmf.aip.org/
Journalists may request a free PDF of this article by contacting firstname.lastname@example.org
Audio clip portions of an interview with one of the researchers are also available. For more details, contact: email@example.com
Biomicrofluidics is an online open-access journal published by the American Institute of Physics to rapidly disseminate research in elucidating fundamental physicochemical mechanisms associated with microfluidic and nanofluidic phenomena as well as novel microfluidic and nanofluidic techniques for diagnostic, medical, biological, pharmaceutical, environmental, and chemical applications. See: http://bmf.aip.org/
The American Institute of Physics is a federation of 10 physical science societies representing more than 135,000 scientists, engineers, and educators and is one of the world's largest publishers of scientific information in the physical sciences. Offering partnership solutions for scientific societies and for similar organizations in science and engineering, AIP is a leader in the field of electronic publishing of scholarly journals. AIP publishes 12 journals (some of which are the most highly cited in their respective fields), two magazines, including its flagship publication Physics Today; and the AIP Conference Proceedings series. Its online publishing platform Scitation hosts nearly two million articles from more than 185 scholarly journals and other publications of 28 learned society publishers.
Jason Socrates Bardi | EurekAlert!
Biomarkers for identifying Tumor Aggressiveness
26.07.2017 | Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft
The dense vessel network regulates formation of thrombocytes in the bone marrow
25.07.2017 | Rudolf-Virchow-Zentrum für Experimentelle Biomedizin der Universität Würzburg
Strong light-matter coupling in these semiconducting tubes may hold the key to electrically pumped lasers
Light-matter quasi-particles can be generated electrically in semiconducting carbon nanotubes. Material scientists and physicists from Heidelberg University...
Fraunhofer IPA has developed a proximity sensor made from silicone and carbon nanotubes (CNT) which detects objects and determines their position. The materials and printing process used mean that the sensor is extremely flexible, economical and can be used for large surfaces. Industry and research partners can use and further develop this innovation straight away.
At first glance, the proximity sensor appears to be nothing special: a thin, elastic layer of silicone onto which black square surfaces are printed, but these...
3-D shape acquisition using water displacement as the shape sensor for the reconstruction of complex objects
A global team of computer scientists and engineers have developed an innovative technique that more completely reconstructs challenging 3D objects. An ancient...
Physicists have developed a new technique that uses electrical voltages to control the electron spin on a chip. The newly-developed method provides protection from spin decay, meaning that the contained information can be maintained and transmitted over comparatively large distances, as has been demonstrated by a team from the University of Basel’s Department of Physics and the Swiss Nanoscience Institute. The results have been published in Physical Review X.
For several years, researchers have been trying to use the spin of an electron to store and transmit information. The spin of each electron is always coupled...
What is the mass of a proton? Scientists from Germany and Japan successfully did an important step towards the most exact knowledge of this fundamental constant. By means of precision measurements on a single proton, they could improve the precision by a factor of three and also correct the existing value.
To determine the mass of a single proton still more accurate – a group of physicists led by Klaus Blaum and Sven Sturm of the Max Planck Institute for Nuclear...
21.07.2017 | Event News
19.07.2017 | Event News
12.07.2017 | Event News
26.07.2017 | Health and Medicine
26.07.2017 | Life Sciences
25.07.2017 | Physics and Astronomy