Alzheimer's, Huntington's, Parkinson's. Names forever linked to what they represent: diseases that ravage the brain's neurons and leave entire regions to wither and die.
These and other so-called neurodegenerative diseases are often associated with the buildup of toxic proteins that lead to the death of neurons. But now, scientists at the Gladstone Institutes have discovered that the progression of disease is not due to the buildup of toxins itself, but rather in the individual neurons' ability to flush these toxins out.
Further, they have identified a therapeutic target that could boost this ability, thereby protecting the brain from the diseases' deadly effects.
In the latest issue of Nature Chemical Biology, researchers in the laboratory of Gladstone Investigator Steve Finkbeiner, MD, PhD, describe how a newly developed technology allowed them to see—for the first time—how individual neurons fight back against the buildup of toxic proteins over time. Focusing their efforts on a model of Huntington's disease, the team observed how different types of neurons in the brain each responded to this toxic buildup with different degrees of success, offering clues as to why the disease causes neurons in one region to die, while neurons in another are spared.
"Huntington's—an inherited and fatal disorder that leads to problems with muscle coordination, cognition and personality—is characterized by the toxic buildup of a mutant form the huntingtin protein in the brain," explained Dr. Finkbeiner, who directs the Taube-Koret Center for Neurodegenerative Disease Research at Gladstone. Dr. Finkbeiner is also a professor of neurology and physiology at the University of California, San Francisco, with which Gladstone is affiliated. "A long-standing mystery among researchers was how the buildup of the mutant huntingtin protein caused cells to degrade and die, but previous technology made it virtually impossible to see and monitor this process at the cellular level. In this study, we employed a method called optical pulse-labeling, or OPL, which allowed us to see how the mutant huntingtin ravaged the brain over time—neuron by neuron."
Using neurons extracted from rodent models of Huntington's, the team employed the OPL method, which monitored the speed and efficiency with which different types of neurons were able to flush out the mutant huntingtin. The faster a cell could clear out the toxins, the longer the neuron survived, and vice versa.
Surprisingly, the research team noticed clear differences in the ability of different types of neurons to clear mutant huntingtin. Neurons located in the striatum—the region of the brain primarily affected by Huntington's disease that is involved in muscle movement—were particularly susceptible. Neurons found in other regions, such as the cortex and cerebellum, were less so. And when they tracked the striatal neurons carrying the mutant huntingtin, they found them much more likely to die than those from other brain regions.
All cells depend on two main processes to clear excess proteins: the ubiquitin-proteasome system (UPS) and autophagy. Although their mechanisms are distinct, their goal is the same: to literally gobble up excess proteins, ensuring they are efficiently flushed out so as to not to interfere with normal cellular activity.
The research team found that striatal neurons were particularly sensitive to disruptions to the autophagy process. But the team found a way around this problem. They artificially accelerated autophagy by boosting the activity of a protein called Nrf2 in these neurons, which prolonged cell survival.
"If we could develop drugs that boost Nrf2 production in the neurons most susceptible to Huntington's, we might extend their survival, thereby staving off the worst effects of the disease," said former Gladstone Postdoctoral Fellow Andrey Tsvetkov, PhD, the study's lead author. "Importantly, our results also demonstrate that the brain itself has evolved powerful coping mechanisms against diseases such as Huntington's. For example, the fact that people don't start experiencing symptoms of Huntington's until the fourth or fifth decade of their lives—even though the mutant huntingtin is present at birth—is further evidence of the brain's ability to stave off the effects of the disease."
"Our findings are critical not only for informing us as to the underlying mechanisms behind diseases such as Huntington's, but also to remind researchers that focusing only on the disease-causing protein—and not how individual cells respond to it–is only one side of the coin," said Dr. Finkbeiner. "To truly understand a complex disease like Huntington's, we must also look to the brain's naturally evolved defense mechanisms, which as we've shown here could represent an entirely new therapeutic strategy."
Montserrat Arrasate, PhD, Sami Barmada, MD, PhD, and Punita Sharma, PhD, also participated in this research at Gladstone, which received support from the National Institute of Neurological Disease and Stroke, the National Institute on Aging, the Huntington's Disease Society of America, the Taube-Koret Center for Neurodegenerative Disease, the Hereditary Disease Foundation, the Hilblom Foundation, the California Institute for Regenerative Medicine, the National Center for Research Resources and the James E. Bashaw Family.
About the Gladstone Institutes
Gladstone is an independent and nonprofit biomedical-research organization dedicated to accelerating the pace of scientific discovery and innovation to prevent, treat and cure cardiovascular, viral and neurological diseases. Gladstone is affiliated with the University of California, San Francisco.
Anne Holden | EurekAlert!
Polymers Based on Boron?
18.01.2018 | Julius-Maximilians-Universität Würzburg
Bioengineered soft microfibers improve T-cell production
18.01.2018 | Columbia University School of Engineering and Applied Science
What enables electrons to be transferred swiftly, for example during photosynthesis? An interdisciplinary team of researchers has worked out the details of how...
For the first time, scientists have precisely measured the effective electrical charge of a single molecule in solution. This fundamental insight of an SNSF Professor could also pave the way for future medical diagnostics.
Electrical charge is one of the key properties that allows molecules to interact. Life itself depends on this phenomenon: many biological processes involve...
At the JEC World Composite Show in Paris in March 2018, the Fraunhofer Institute for Laser Technology ILT will be focusing on the latest trends and innovations in laser machining of composites. Among other things, researchers at the booth shared with the Aachen Center for Integrative Lightweight Production (AZL) will demonstrate how lasers can be used for joining, structuring, cutting and drilling composite materials.
No other industry has attracted as much public attention to composite materials as the automotive industry, which along with the aerospace industry is a driver...
Scientists at Tokyo Institute of Technology (Tokyo Tech) and Tohoku University have developed high-quality GFO epitaxial films and systematically investigated their ferroelectric and ferromagnetic properties. They also demonstrated the room-temperature magnetocapacitance effects of these GFO thin films.
Multiferroic materials show magnetically driven ferroelectricity. They are attracting increasing attention because of their fascinating properties such as...
The oceans are the largest global heat reservoir. As a result of man-made global warming, the temperature in the global climate system increases; around 90% of...
08.01.2018 | Event News
11.12.2017 | Event News
08.12.2017 | Event News
18.01.2018 | Life Sciences
18.01.2018 | Life Sciences
18.01.2018 | Earth Sciences