Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

F is for Fluoresence and Fluorine

29.03.2010
New dyes for optical nanoscopy

The imaging of living cells at the molecular level was barely a dream twenty years ago. Today, however, this dream is close to becoming reality. In the Max Planck Institute for NanoBiophotonics in Göttingen, Stefan Hell (2009 recipient of the Otto Hahn Prize) has developed fluorescence microscopy methods for observing objects on the nanoscale and with his colleagues Vladimir Belov and Christian Eggeling a new series of photostable dyes that can be used as fluorescent markers has been realised, as reported in a cover story in Chemistry—A European Journal.

Over the last two decades Stefan Hell and his group have revolutionized the art of microscopy beyond limits thought to have been unbreakable. Due to the wave properties (diffraction) of light, the resolution of an optical microscope is limited to object details of about 0.2 micrometers. The laws of physics appeared to prohibit imaging details beyond this limit. Stefan Hell saw beyond this limitation and about fifteen years ago his vision became concrete; he developed a method for observing objects at the nanometer scale by sequentially turning the fluorescence of nearby molecules off by stimulated emission, a technique known as STED nanoscopy.

The sensitivity of this technique depends on the brightness of the applied fluorescence markers and their photostability is also of great importance. The NanoBiophotonics group has succeeded in synthesizing a series of highly photostable and highly fluorescent dyes. These compounds emit green and orange light and are based on fluorine derivatives of the well-known Rhodamine dye. The use of these dyes in STED nanoscopy leads to images of high-quality with respect to brightness and signal-to-background ratio; further the resolution over that of more traditional optical microscopes is significantly improved giving more detailed structural information.

These rhodamine-based fluorine derivatives are even more special because of their versatility. The compounds are available in hydrophilic and lipophilic forms, and with the inclusion of amino reactive groups, they can be easily attached to antibodies or other biomolecules in the course of standard labeling and immunostaining procedures. The group demonstrate that these new dyes are able to cross cellular membranes and reach the interior of living cells, which could lead to new labeling strategies for biological systems. All eyes are now on Göttingen to see just how far optical nanoscopy can go.

Author: Stefan Hell, Max Planck Institute of NanoBiophotonics, Göttingen (Germany), http://www.mpibpc.mpg.de/groups/hell/

Title: New Fluorinated Rhodamines for Optical Microscopy and Nanoscopy

Chemistry - A European Journal 2010, 16, No. 15, 4477–4488, Permalink to the article: http://dx.doi.org/10.1002/chem.200903272

Stefan Hell | Wiley-VCH
Further information:
http://www.chemeurj.org
http://www.mpibpc.mpg.de/groups/hell/

More articles from Life Sciences:

nachricht Enduring cold temperatures alters fat cell epigenetics
19.04.2018 | University of Tokyo

nachricht Full of hot air and proud of it
18.04.2018 | University of Pittsburgh

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Gamma-ray flashes from plasma filaments

Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.

The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...

Im Focus: Basel researchers succeed in cultivating cartilage from stem cells

Stable joint cartilage can be produced from adult stem cells originating from bone marrow. This is made possible by inducing specific molecular processes occurring during embryonic cartilage formation, as researchers from the University and University Hospital of Basel report in the scientific journal PNAS.

Certain mesenchymal stem/stromal cells from the bone marrow of adults are considered extremely promising for skeletal tissue regeneration. These adult stem...

Im Focus: Like a wedge in a hinge

Researchers lay groundwork to tailor drugs for new targets in cancer therapy

In the fight against cancer, scientists are developing new drugs to hit tumor cells at so far unused weak points. Such a “sore spot” is the protein complex...

Im Focus: The Future of Ultrafast Solid-State Physics

In an article that appears in the journal “Review of Modern Physics”, researchers at the Laboratory for Attosecond Physics (LAP) assess the current state of the field of ultrafast physics and consider its implications for future technologies.

Physicists can now control light in both time and space with hitherto unimagined precision. This is particularly true for the ability to generate ultrashort...

Im Focus: Stronger evidence for a weaker Atlantic overturning

The Atlantic overturning – one of Earth’s most important heat transport systems, pumping warm water northwards and cold water southwards – is weaker today than any time before in more than 1000 years. Sea surface temperature data analysis provides new evidence that this major ocean circulation has slowed down by roughly 15 percent since the middle of the 20th century, according to a study published in the highly renowned journal Nature by an international team of scientists. Human-made climate change is a prime suspect for these worrying observations.

“We detected a specific pattern of ocean cooling south of Greenland and unusual warming off the US coast – which is highly characteristic for a slowdown of the...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Invitation to the upcoming "Current Topics in Bioinformatics: Big Data in Genomics and Medicine"

13.04.2018 | Event News

Unique scope of UV LED technologies and applications presented in Berlin: ICULTA-2018

12.04.2018 | Event News

IWOLIA: A conference bringing together German Industrie 4.0 and French Industrie du Futur

09.04.2018 | Event News

 
Latest News

Improved stability of plastic light-emitting diodes

19.04.2018 | Power and Electrical Engineering

Enduring cold temperatures alters fat cell epigenetics

19.04.2018 | Life Sciences

New capabilities at NSLS-II set to advance materials science

18.04.2018 | Materials Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>