They explode the cell while it is still living inside a plant or animal, vaporize its contents, and sniff. The study appears in online in ACS’ journal Analytical Chemistry: “In Situ Metabolic Profiling of Single Cells by Laser Ablation Electrospray Ionization Mass Spectrometry”.
Akos Vertes and Bindesh Shrestha note that knowing the contents of cells is the key to understanding how healthy cells differ from those in disease. Until now, however, the only way to “look” inside an individual cell was to remove it from its natural environment in an animal or plant, or change its environment. But doing so changed the cell. Scientists never knew whether one cell differed from another because of the disease, or because they had removed it to a new environment.
The new report describes development of a new technique that uses laser pulses focused through a tiny glass fiber to explode a cell and turn its contents into vapor. Scientists then use a laboratory instrument to analyze the vapor and get a profile of the chemicals inside. It can reveal differences between diseased and healthy cells, even between adjacent cells in the same tissue. The scientists used this new technique to analyze the contents of living plant and animal cells and show that it quickly and accurately identified important chemical details that would have been overlooked using conventional techniques.
“In Situ Metabolic Profiling of Single Cells by Laser Ablation Electrospray Ionization Mass Spectrometry”ARTICLE FOR IMMEDIATE RELEASE:
Michael Woods | Newswise Science News
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‘Spying’ on the hidden geometry of complex networks through machine intelligence
08.12.2017 | Technische Universität Dresden
Tiny pores at a cell's entryway act as miniature bouncers, letting in some electrically charged atoms--ions--but blocking others. Operating as exquisitely sensitive filters, these "ion channels" play a critical role in biological functions such as muscle contraction and the firing of brain cells.
To rapidly transport the right ions through the cell membrane, the tiny channels rely on a complex interplay between the ions and surrounding molecules,...
The miniaturization of the current technology of storage media is hindered by fundamental limits of quantum mechanics. A new approach consists in using so-called spin-crossover molecules as the smallest possible storage unit. Similar to normal hard drives, these special molecules can save information via their magnetic state. A research team from Kiel University has now managed to successfully place a new class of spin-crossover molecules onto a surface and to improve the molecule’s storage capacity. The storage density of conventional hard drives could therefore theoretically be increased by more than one hundred fold. The study has been published in the scientific journal Nano Letters.
Over the past few years, the building blocks of storage media have gotten ever smaller. But further miniaturization of the current technology is hindered by...
With innovative experiments, researchers at the Helmholtz-Zentrums Geesthacht and the Technical University Hamburg unravel why tiny metallic structures are extremely strong
Light-weight and simultaneously strong – porous metallic nanomaterials promise interesting applications as, for instance, for future aeroplanes with enhanced...
An interdisciplinary group of researchers interfaced individual bacteria with a computer to build a hybrid bio-digital circuit - Study published in Nature Communications
Scientists at the Institute of Science and Technology Austria (IST Austria) have managed to control the behavior of individual bacteria by connecting them to a...
Physicists in the Laboratory for Attosecond Physics (run jointly by LMU Munich and the Max Planck Institute for Quantum Optics) have developed an attosecond electron microscope that allows them to visualize the dispersion of light in time and space, and observe the motions of electrons in atoms.
The most basic of all physical interactions in nature is that between light and matter. This interaction takes place in attosecond times (i.e. billionths of a...
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