Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Establishing the first line of human embryonic stem cells in Brazil

03.05.2011
Brazilian researchers, reporting in the current issue of Cell Transplantation (20:3) (now freely available on-line at http://www.ingentaconnect.com/content/cog/ct/ ), discovered difficulties in establishing a genetically diverse line of human embryonic stem cells (hES) to serve the therapeutic stem cell transplantation needs of the diverse ethnic and genetic Brazilian population.

According to the study's corresponding author, Dr. Lygia V. Pereira of the Molecular Genetics Laboratory at the University of Sao Paulo, Brazil, pluripotent human embryonic stem cells are an important tool for basic and applied stem cell transplantation research. However, immunocompatibility is an issue, especially in a genetically diverse population such as that in Brazil where the population is comprised of European, African and Native South American ancestry.

In their study, the researchers developed an hES cell line (the first in South America) they called "BR-1" derived from a Brazilian population with embryos donated by couples who had sought assistance from private fertility clinics. Their research was compatible with the 2005 Brazilian national legal, ethical and clinical guidelines for embryonic stem cell research using tissue that had been frozen for the legally mandated time of at least three years, and that had been produced for reproductive reasons.

Their results showed that the hES cell lines they established were a "worse match" to the Brazilian population than hES lines developed elsewhere, particularly those developed in the U.S. and Singapore. The reasons for that may be several, said Dr. Pereira.

"The Brazilian population is one of the most heterogeneous in the world, and the genes of Brazilians are mosaics," said Dr. Pereira. "However, an analysis of BR-1 showed that it is mostly European in origin. The reproductive assistance offered by the Brazilian public health system does not include cryopreservation of surplus embryos, meaning that the only research material available came from private clinics where couples with above-average incomes could afford the high cost of assisted reproduction."

According to the researchers, that segment of the Brazilian population is mostly composed of people self-identified as white - of European ancestry - and so are not representative of Brazilian ethnic admixture and, thus, are unable to be widely compatible with Brazil's diverse population genetics.

"Although we have successfully established the first line of hES from the Brazilian population that adds to the pool of genetically different pluripotent cells available, it will be important to have access to embryos from the more mixed population and assistance from the Public Health System," concluded Dr. Pereira.

"Use of embryonic stem cells (ES) in regenerative medicine is very promising, but the potential problems of tumor development, cell rejection due to histo-incompatibility, and contamination with animal products employed in the cell culture need to be overcome," said Dr. Julio Voltarelli, professor of Clinical Medicine and Clinical Immunology at the University of Sao Pãulo, Brazil and section editor for Cell Transplantation. "In this study, Dr Pereira and colleagues compared the HLA compatibility between their ES line, the first established in Brazil, and a sample of the Brazilian population who volunteered as donors for hematopoietic stem cell transplantation (REDOME). They found few matches for the ES line in the representative population, which was attributed to the great genetic heterogeneity of the Brazilian population. This finding may add another difficulty to the clinical use of ES in Brazil and other mixed populations even once the safety issues of ES lines are resolved."

Contact: Dr. Lygia V. Pereira, Laboratorio de Genetica Molecular and Laboratorio Nacional de Celuas-Tronco Embrionarias, Instituto de Biociensas, Universidade de Sao Paulo, Sao Paulo SP, 05508-090 Brasil,

Tel: Fax: Email: lpereira@usp.br

Citation:. Fraga, A. M.; Sukoyan, M.; Rajan, P.; Braga, D. P. F.; Iaconelli, A., Jr.; Franco, J. G., Jr.; Borges, E., Jr.; Pereira, L. V. Establishment Of A Brazilian Line Of Human Embryonic Stem Cells In Defined Medium – Implications For Cell Therapy In An Ethnically Diverse Population. Cell Transplant. 20(3):431-440; 2011.

The editorial offices for Cell Transplantation are at the Center of Excellence for Aging and Brain Repair, College of Medicine, the University of South Florida and the Diabetes Research Institute, University of Miami Miller School of Medicine. Contact, David Eve, PhD. at celltransplantation@gmail.com or Camillo Ricordi, MD at ricordi@miami.edu

News Release by Randolph Fillmore, Florida Science Communications, www.sciencescribe.net

David Eve | EurekAlert!
Further information:
http://www.ingentaconnect.com/content/cog/ct/

More articles from Life Sciences:

nachricht At last, butterflies get a bigger, better evolutionary tree
16.02.2018 | Florida Museum of Natural History

nachricht New treatment strategies for chronic kidney disease from the animal kingdom
16.02.2018 | Veterinärmedizinische Universität Wien

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Demonstration of a single molecule piezoelectric effect

Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale

Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...

Im Focus: Hybrid optics bring color imaging using ultrathin metalenses into focus

For photographers and scientists, lenses are lifesavers. They reflect and refract light, making possible the imaging systems that drive discovery through the microscope and preserve history through cameras.

But today's glass-based lenses are bulky and resist miniaturization. Next-generation technologies, such as ultrathin cameras or tiny microscopes, require...

Im Focus: Stem cell divisions in the adult brain seen for the first time

Scientists from the University of Zurich have succeeded for the first time in tracking individual stem cells and their neuronal progeny over months within the intact adult brain. This study sheds light on how new neurons are produced throughout life.

The generation of new nerve cells was once thought to taper off at the end of embryonic development. However, recent research has shown that the adult brain...

Im Focus: Interference as a new method for cooling quantum devices

Theoretical physicists propose to use negative interference to control heat flow in quantum devices. Study published in Physical Review Letters

Quantum computer parts are sensitive and need to be cooled to very low temperatures. Their tiny size makes them particularly susceptible to a temperature...

Im Focus: Autonomous 3D scanner supports individual manufacturing processes

Let’s say the armrest is broken in your vintage car. As things stand, you would need a lot of luck and persistence to find the right spare part. But in the world of Industrie 4.0 and production with batch sizes of one, you can simply scan the armrest and print it out. This is made possible by the first ever 3D scanner capable of working autonomously and in real time. The autonomous scanning system will be on display at the Hannover Messe Preview on February 6 and at the Hannover Messe proper from April 23 to 27, 2018 (Hall 6, Booth A30).

Part of the charm of vintage cars is that they stopped making them long ago, so it is special when you do see one out on the roads. If something breaks or...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

2nd International Conference on High Temperature Shape Memory Alloys (HTSMAs)

15.02.2018 | Event News

Aachen DC Grid Summit 2018

13.02.2018 | Event News

How Global Climate Policy Can Learn from the Energy Transition

12.02.2018 | Event News

 
Latest News

Fingerprints of quantum entanglement

16.02.2018 | Information Technology

'Living bandages': NUST MISIS scientists develop biocompatible anti-burn nanofibers

16.02.2018 | Health and Medicine

Hubble sees Neptune's mysterious shrinking storm

16.02.2018 | Physics and Astronomy

VideoLinks
Science & Research
Overview of more VideoLinks >>>