With this method the researchers were able to prove for the first time that not only cell-intrinsic mechanisms, but also external environmental factors such as growth factors can control HPC lineage choice directly.
"This is simply because until now we did not have the suitable technology to observe the processes of cell differentiation and to measure them quantitatively," explained Dr. Timm Schroeder, research group leader at the Institute of Stem Cell Research of Helmholtz Zentrum München. "We didn’t know exactly what happens during this time span," Dr. Schroeder said. “That is why until now we could not prove what role cytokines play.”
With the new bioimaging techniques developed by Dr. Schroeder’s team, progenitor cells could be observed for a longer period and on the single-cell level. Depending on the kind of cytokines present, after a few days the HPC cultures contained only one cell type. The question remained unanswered whether this was a consequence of direct cytokine regulation or merely the result of sorting out “erroneously differentiated” cells by cell death. Using the new bioimaging techniques for continuous single-cell observation, Dr. Michael Rieger and students in Dr. Schroeder’s research group showed for the first time that no cell death could be detected during the entire cell differentiation process. This proves unambiguously that HPC lineage choices can be steered by external environmental factors such as in this case by cytokines. The hematopoietic progenitor cells are “instructed” by cytokines.
"These findings confirm that signaling pathways that are activated by cytokine receptors influence the lineage choices of the cells,” Dr. Schroeder said. “The new method offers us the unique chance to observe the effect of all the molecules involved in the differentiation process separately and to better understand their role. This is an important requirement for optimizing the therapeutic use of stem cells.”Further information
Helmholtz Zentrum München is the German Research Center for Environmental Health. As leading center oriented toward Environmental Health, it focuses on chronic and complex diseases which develop from the interaction of environmental factors and individual genetic disposition. Helmholtz Zentrum München has around 1680 staff members. The head office of the center is located in Neuherberg to the north of Munich on a 50-hectare research campus. Helmholtz Zentrum München belongs to the Helmholtz Association, Germany’s largest research organization, a community of 16 scientific-technical and medical-biological research centers with a total of 26,500 staff members.Editor
Sven Winkler | EurekAlert!
Enduring cold temperatures alters fat cell epigenetics
19.04.2018 | University of Tokyo
Full of hot air and proud of it
18.04.2018 | University of Pittsburgh
Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.
Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...
Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.
The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...
Stable joint cartilage can be produced from adult stem cells originating from bone marrow. This is made possible by inducing specific molecular processes occurring during embryonic cartilage formation, as researchers from the University and University Hospital of Basel report in the scientific journal PNAS.
Certain mesenchymal stem/stromal cells from the bone marrow of adults are considered extremely promising for skeletal tissue regeneration. These adult stem...
In the fight against cancer, scientists are developing new drugs to hit tumor cells at so far unused weak points. Such a “sore spot” is the protein complex...
In an article that appears in the journal “Review of Modern Physics”, researchers at the Laboratory for Attosecond Physics (LAP) assess the current state of the field of ultrafast physics and consider its implications for future technologies.
Physicists can now control light in both time and space with hitherto unimagined precision. This is particularly true for the ability to generate ultrashort...
13.04.2018 | Event News
12.04.2018 | Event News
09.04.2018 | Event News
19.04.2018 | Materials Sciences
19.04.2018 | Physics and Astronomy
19.04.2018 | Physics and Astronomy