Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Environmental factors instruct lineage choice of blood progenitor cells

17.07.2009
The research team led by Dr. Timm Schroeder, stem cell researcher at Helmholtz Zentrum München, has developed a new bioimaging method for observing the differentiation of hematopoietic progenitor cells (HPC) at the single-cell level.

With this method the researchers were able to prove for the first time that not only cell-intrinsic mechanisms, but also external environmental factors such as growth factors can control HPC lineage choice directly.

The findings, published in the current issue of the prestigious journal Science, provide an essential building block for understanding the molecular mechanisms of hematopoiesis and are an important prerequisite for optimizing therapeutic stem cell applications.

For decades scientists from various disciplines have discussed to what respective extent genetic predisposition and environmental factors influence human development. Likewise, the same ‘nature vs. nurture’ issue is debated by hematologists and stem cell researchers: Is multipotent progenitor cell differentiation influenced solely by cell-intrinsic mechanisms or is it also influenced by the environment of the cell? What role do growth factors such as cytokines play? Do they influence HPC lineage choice directly or do they merely regulate the survival of the cell after the lineage choice has been made? Despite the immense importance of cytokines for day-to-day clinical research – and not least their far-reaching significance commercially - this issue has been one of the key unknown factors in the stem cell biology of blood.

"This is simply because until now we did not have the suitable technology to observe the processes of cell differentiation and to measure them quantitatively," explained Dr. Timm Schroeder, research group leader at the Institute of Stem Cell Research of Helmholtz Zentrum München. "We didn’t know exactly what happens during this time span," Dr. Schroeder said. “That is why until now we could not prove what role cytokines play.”

With the new bioimaging techniques developed by Dr. Schroeder’s team, progenitor cells could be observed for a longer period and on the single-cell level. Depending on the kind of cytokines present, after a few days the HPC cultures contained only one cell type. The question remained unanswered whether this was a consequence of direct cytokine regulation or merely the result of sorting out “erroneously differentiated” cells by cell death. Using the new bioimaging techniques for continuous single-cell observation, Dr. Michael Rieger and students in Dr. Schroeder’s research group showed for the first time that no cell death could be detected during the entire cell differentiation process. This proves unambiguously that HPC lineage choices can be steered by external environmental factors such as in this case by cytokines. The hematopoietic progenitor cells are “instructed” by cytokines.

"These findings confirm that signaling pathways that are activated by cytokine receptors influence the lineage choices of the cells,” Dr. Schroeder said. “The new method offers us the unique chance to observe the effect of all the molecules involved in the differentiation process separately and to better understand their role. This is an important requirement for optimizing the therapeutic use of stem cells.”

Further information
Original publication:
Rieger MA, Hoppe PS, Smejkal BM, Eitelhuber AC & Schroeder T (2009): Hematopoietic cytokines can instruct lineage choice. Science 325:217-218
Directed by Professor Magdalena Götz, the Institute of Stem Cell Research at Helmholtz Zentrum München combines research on stem cells of the nervous system, the hematopoietic system and of the endoderm. Especially in view of clinical applications, the objective of the Institute is to elucidate the underlying mechanisms for the specification of stem cells in order to utilize these in a targeted manner to repair damaged cells.

Further information...

Helmholtz Zentrum München is the German Research Center for Environmental Health. As leading center oriented toward Environmental Health, it focuses on chronic and complex diseases which develop from the interaction of environmental factors and individual genetic disposition. Helmholtz Zentrum München has around 1680 staff members. The head office of the center is located in Neuherberg to the north of Munich on a 50-hectare research campus. Helmholtz Zentrum München belongs to the Helmholtz Association, Germany’s largest research organization, a community of 16 scientific-technical and medical-biological research centers with a total of 26,500 staff members.

Editor
Sven Winkler, Helmholtz Zentrum München – German Research Center for Environmental Health, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany. Phone: +49(0)89-3187-3946. Fax +49(0)89-3187-3324, email: presse@helmholtz-muenchen.de

Sven Winkler | EurekAlert!
Further information:
http://www.helmholtz-muenchen.de

More articles from Life Sciences:

nachricht Enduring cold temperatures alters fat cell epigenetics
19.04.2018 | University of Tokyo

nachricht Full of hot air and proud of it
18.04.2018 | University of Pittsburgh

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Writing and deleting magnets with lasers

Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.

Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...

Im Focus: Gamma-ray flashes from plasma filaments

Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.

The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...

Im Focus: Basel researchers succeed in cultivating cartilage from stem cells

Stable joint cartilage can be produced from adult stem cells originating from bone marrow. This is made possible by inducing specific molecular processes occurring during embryonic cartilage formation, as researchers from the University and University Hospital of Basel report in the scientific journal PNAS.

Certain mesenchymal stem/stromal cells from the bone marrow of adults are considered extremely promising for skeletal tissue regeneration. These adult stem...

Im Focus: Like a wedge in a hinge

Researchers lay groundwork to tailor drugs for new targets in cancer therapy

In the fight against cancer, scientists are developing new drugs to hit tumor cells at so far unused weak points. Such a “sore spot” is the protein complex...

Im Focus: The Future of Ultrafast Solid-State Physics

In an article that appears in the journal “Review of Modern Physics”, researchers at the Laboratory for Attosecond Physics (LAP) assess the current state of the field of ultrafast physics and consider its implications for future technologies.

Physicists can now control light in both time and space with hitherto unimagined precision. This is particularly true for the ability to generate ultrashort...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Invitation to the upcoming "Current Topics in Bioinformatics: Big Data in Genomics and Medicine"

13.04.2018 | Event News

Unique scope of UV LED technologies and applications presented in Berlin: ICULTA-2018

12.04.2018 | Event News

IWOLIA: A conference bringing together German Industrie 4.0 and French Industrie du Futur

09.04.2018 | Event News

 
Latest News

Diamond-like carbon is formed differently to what was believed -- machine learning enables development of new model

19.04.2018 | Materials Sciences

Electromagnetic wizardry: Wireless power transfer enhanced by backward signal

19.04.2018 | Physics and Astronomy

Ultrafast electron oscillation and dephasing monitored by attosecond light source

19.04.2018 | Physics and Astronomy

VideoLinks
Science & Research
Overview of more VideoLinks >>>