Study published today in Science sheds light on structure of nuclear pore complex, which plays a crucial role in controlling molecular traffic to a cell's nucleus
It was a 3D puzzle with over 1000 pieces, with only a rather fuzzy outline as a guide. But scientists at EMBL have now put enough pieces in place to see the big picture. In a study published today in Science, they present their latest findings, bringing the nuclear pore complex into focus.
The nuclear pore is a passage into the cell's nucleus. A typical cell has hundreds of these pores, playing a crucial role in controlling the hundred of thousands of molecules that enter and exit this compartment every minute. Nuclear pores are used by many viruses to inject their genetic material into a host and they are known to change when cells become cancerous, so knowing how they work is important. Scientists understood many of the components of the nuclear pore, but exactly how those building blocks fitted together was unclear.
"The nuclear pore is the biggest, most complicated protein complex in a human cell. We now understand how it is structured," says Martin Beck, who led the work at EMBL. "This is a very important first step towards understanding what actually happens to nuclear pores in cancer, during ageing, and in other conditions."
The nuclear pore is composed of three layered rings: a nuclear ring facing the nucleus; a cytoplasmic ring facing the rest of the cell; and an inner ring in between those two. Having already pieced together how the building blocks of the nuclear and cytoplasmic rings are arranged, Martin Beck's group at EMBL have now worked out the arrangement of the pieces that form the inner ring.
"Surprisingly, we found that although it is made of different building blocks, the inner ring has the same basic architecture as the other two rings," says Shyamal Mosalaganti from EMBL, who studied the ring using cryo-electron microscopy. "This very complicated structure is built using simple principles . We were able to uncover that because we interweaved a lot of different techniques here."
Beck and colleagues would now like to understand how the cell completes this 3D puzzle: how the nuclear pore is assembled. Thanks to an ERC grant, the scientists are also exploring how the pore varies between different kinds of cells.
"Knowing this structure, we can now look at how nuclear pores evolved, right at the beginning of the development of complex cells," says Jan Kosinski from EMBL, who put the pieces together using computer modelling. "The moment you have a nucleus -- a compartment which encloses DNA -- you need a nuclear pore, but how it developed is still the subject of intense debate."
The work was carried out in collaboration with Ed Hurt's lab at the University of Heidelberg, Germany, and Joseph Glavy's lab at the Stevens Institute of Technology, USA.
Isabelle Kling | EurekAlert!
One step closer to reality
20.04.2018 | Max-Planck-Institut für Entwicklungsbiologie
The dark side of cichlid fish: from cannibal to caregiver
20.04.2018 | Veterinärmedizinische Universität Wien
University of Connecticut researchers have created a biodegradable composite made of silk fibers that can be used to repair broken load-bearing bones without the complications sometimes presented by other materials.
Repairing major load-bearing bones such as those in the leg can be a long and uncomfortable process.
Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.
Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...
Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.
The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...
Stable joint cartilage can be produced from adult stem cells originating from bone marrow. This is made possible by inducing specific molecular processes occurring during embryonic cartilage formation, as researchers from the University and University Hospital of Basel report in the scientific journal PNAS.
Certain mesenchymal stem/stromal cells from the bone marrow of adults are considered extremely promising for skeletal tissue regeneration. These adult stem...
In the fight against cancer, scientists are developing new drugs to hit tumor cells at so far unused weak points. Such a “sore spot” is the protein complex...
13.04.2018 | Event News
12.04.2018 | Event News
09.04.2018 | Event News
20.04.2018 | Physics and Astronomy
20.04.2018 | Interdisciplinary Research
20.04.2018 | Physics and Astronomy