Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Donor Cells for Immune Therapy

23.10.2008
In the future, bone marrow transfer to patients with leukaemia could be more secure. Experiments with mice have shown that certain cells of the immune system (regulatory T cells or Tregs) can suppress the dangerous side effects resulting from treatment.

Such cells control aggressive immune cells and, thus, unwanted immune reactions by the graft can be avoided. However, to date, there were no adequate techniques available to securely isolate the regulatory T cells.

Now, researchers of the Max Delbrück Center (MDC), Germany and their colleagues of the 'Fondazione Santa Lucia' in Rome, Italy have developed a simple method to specifically isolate these cells from human blood. (Blood)*.

According to the American Cancer Society, about 188,000 people world-wide developed leukaemia in 2007. Predominantly immature white blood cells can be found in their blood. These cells displace the healthy cells and, thus, suppress Blood a normal haematopoiesis. Chemotherapy destroys the diseased cells of the patient which then often have to be replaced by a bone marrow graft.

"However," says Dr. Markus Kleinewietfeld (MDC), "in 30 to 50 percent of the patients, the aggressive immune cells contaminating the bone marrow graft direct themselves against the recipient." This often lethal defence reaction is called 'Graft versus Host Disease' (GvHD). Yet, with the help of regulatory T cells from the blood of the donor, the rejection reaction might be suppressed.

"Until now, it was not possible to securely isolate regulatory T cells in their pure form," explains Dr. Kleinewietfeld. Since, in humans, the cell surface marker (CD25) used for the isolation before is also found on the aggressive immune cells, it was hardly possible to clearly separate the helpful Tregs from the dangerous cells.

With other markers (CD49d and CD127), the scientists now succeeded in separating the aggressive and dangerous immune cells from the helpful, regulatory cells. Thus, it is now possible to isolate regulatory T cells in high purity safely from human blood. Using these T cells, the researchers could already suppress a particular severe form of the 'Graft versus Host Disease' in mice. In a first clinical trial in Singapore, the MDC researchers now want to apply the regulatory cells in leukaemia patients who developed the severe immune reaction after a bone marrow donation.

"Singapore provides the infrastructure and financial commitment necessary for such trials," says Dr. Olaf Rötzschke, a former MDC researcher who now works at the 'Singapore Immunology Network' (SIgN) of the BIOPOLIS Campus. "Dependent on the outcome of this clinical test, regulatory T cells could possibly be used also for the treatment of autoimmune diseases, allergies and tissue rejections in the future," hopes Dr. Kirsten Falk, head of the MDC research group.

*Blood: doi 10 1182/blood-2008-04-150524

CD49d provides access to 'untouched' human Foxp3+ Treg free of contaminating effector cells

Markus Kleinewietfeld1, Mireille Starke1, Diletta Di Mitri2, Giovanna Borsellino2, Luca Battistini2, Olaf Rötzschke1,3, Kirsten Falk1

1Max-Delbrück-Center for Molecular Medicine (MDC), Robert-Rössle-Str. 10, D-13125 Berlin, Germany
2Laboratory of Neuroimmunology, Fondazione Santa Lucia, Via del Fosso di Fiorano 65, 00143 Rome, Italy

3Singapore Immunology Network (SIgN), 8A Biomedical Grove, IMMUNOS, Singapore 138648, Singapore

Barbara Bachtler
Press and Public Affairs
Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch
Robert-Rössle-Str. 10?13125 Berlin, Germany
Phone: +49 (0) 30 94 06 - 38 96; Fax: +49 (0) 30 94 06 - 38 33
e-mail: presse@mdc-berlin.de

Barbara Bachtler | idw
Further information:
http://www.mdc-berlin.de/en/news

Further reports about: Isolate MDC Molecular Singapore T cells TREG blood cell blood flow immune cell marrow regulatory

More articles from Life Sciences:

nachricht Warming ponds could accelerate climate change
21.02.2017 | University of Exeter

nachricht An alternative to opioids? Compound from marine snail is potent pain reliever
21.02.2017 | University of Utah

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Breakthrough with a chain of gold atoms

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

Im Focus: DNA repair: a new letter in the cell alphabet

Results reveal how discoveries may be hidden in scientific “blind spots”

Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...

Im Focus: Dresdner scientists print tomorrow’s world

The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.

The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...

Im Focus: Mimicking nature's cellular architectures via 3-D printing

Research offers new level of control over the structure of 3-D printed materials

Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...

Im Focus: Three Magnetic States for Each Hole

Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".

Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Booth and panel discussion – The Lindau Nobel Laureate Meetings at the AAAS 2017 Annual Meeting

13.02.2017 | Event News

Complex Loading versus Hidden Reserves

10.02.2017 | Event News

International Conference on Crystal Growth in Freiburg

09.02.2017 | Event News

 
Latest News

Impacts of mass coral die-off on Indian Ocean reefs revealed

21.02.2017 | Earth Sciences

Novel breast tomosynthesis technique reduces screening recall rate

21.02.2017 | Medical Engineering

Use your Voice – and Smart Homes will “LISTEN”

21.02.2017 | Trade Fair News

VideoLinks
B2B-VideoLinks
More VideoLinks >>>