Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Development of antibiotic resistance more predictable than expected

05.07.2012
New research approach can help predict the ‘tenability’ of antibiotics

Research by Wageningen University, part of Wageningen UR, has shown that the development of bacteria with resistance against the antibiotic cefotaxime occurs more often and more predictably than was previously assumed.


3D enzyme. The 48 mutations found in the enzyme TEM-1 beta-lactamase that increase resistance against the antibiotic cefotaxime. The colours indicate the increase in cefotaxime. The inset shows the same enzyme, turned 180 degrees horizontally.

Bacterial populations were found to have many mutations that increase resistance and therefore have a negative effect on public health. Moreover, the effects are such that it can be predicted that the development of bacterial strains with a resistance against cefotaxime will progress in a similar way in different patients from different locations.

Together with German colleagues, the Wageningen scientists developed a research approach which will allow them to predict whether, and if so how, resistant bacterial strains will develop for other antibiotics as well.

The Wageningen scientists studied the main enzyme that causes resistance against the antibiotic cefotaxime. The only function of this beta-lactamase enzyme is the breakdown of so-called beta-lactam antibiotics, which kill bacteria by preventing the production of their cell walls. Martijn Schenk and Arjan de Visser, genetic scientists at Wageningen University, were surprised by the number of mutations with a positive effect on the resistance against cefotaxime. De Visser: “Of all the mutations we found in this beta-lactamase, more than three per cent caused an increase in the resistance against the antibiotic. To top it all off, we discovered that the mutations with a strong effect also had a much greater impact than we had anticipated. Based on theoretical arguments and previous observations, we had estimated the effects on the resistance against the antibiotic to be significantly lower.”

The presence in particular of mutations with a very strong effect on resistance to the antibiotic facilitates the prediction of the development of resistant bacterial strains.

Collaboration with a group of physicists in Germany enabled the Wageningen scientists to study the genetic findings quantitatively, as Martijn Schenk explains: “The physicists built computer models that helped us as geneticists to move forward. We were able to show that it is probable that the bacteria will become resistant against the antibiotic in a similar way in various patients throughout the world.”

According to De Visser the approach taken can also be used to predict the ‘tenability’ of other antibiotics, as the combination of computer models with knowledge about the number and effect of the mutations provides concrete leads.

http://www.wur.nl/UK/newsagenda/news/PPSG_bacteria_resistance.htm

Attached files

3D enzyme. The 48 mutations found in the enzyme TEM-1 beta-lactamase that increase resistance against the antibiotic cefotaxime. The colours indicate the increase in cefotaxime. The inset shows the same enzyme, turned 180 degrees horizontally.

Table. The Wageningen scientists found an unexpectedly large amount of mutations that considerably increased the resistance of the enzyme to the antibiotic.

Full bibliographic informationSchenk, MF, IG Szendro, J Krug and JAGM de Visser. 2012. Quantifying the adaptive potential of an antibiotic resistance enzyme. PLoS Genetics 8(6): e1002783

Jac Niessen | alfa
Further information:
http://www.wur.nl

More articles from Life Sciences:

nachricht What happens in the cell nucleus after fertilization
06.12.2016 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt

nachricht Researchers uncover protein-based “cancer signature”
05.12.2016 | Universität Basel

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Significantly more productivity in USP lasers

In recent years, lasers with ultrashort pulses (USP) down to the femtosecond range have become established on an industrial scale. They could advance some applications with the much-lauded “cold ablation” – if that meant they would then achieve more throughput. A new generation of process engineering that will address this issue in particular will be discussed at the “4th UKP Workshop – Ultrafast Laser Technology” in April 2017.

Even back in the 1990s, scientists were comparing materials processing with nanosecond, picosecond and femtosesecond pulses. The result was surprising:...

Im Focus: Shape matters when light meets atom

Mapping the interaction of a single atom with a single photon may inform design of quantum devices

Have you ever wondered how you see the world? Vision is about photons of light, which are packets of energy, interacting with the atoms or molecules in what...

Im Focus: Novel silicon etching technique crafts 3-D gradient refractive index micro-optics

A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.

Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...

Im Focus: Quantum Particles Form Droplets

In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.

“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...

Im Focus: MADMAX: Max Planck Institute for Physics takes up axion research

The Max Planck Institute for Physics (MPP) is opening up a new research field. A workshop from November 21 - 22, 2016 will mark the start of activities for an innovative axion experiment. Axions are still only purely hypothetical particles. Their detection could solve two fundamental problems in particle physics: What dark matter consists of and why it has not yet been possible to directly observe a CP violation for the strong interaction.

The “MADMAX” project is the MPP’s commitment to axion research. Axions are so far only a theoretical prediction and are difficult to detect: on the one hand,...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ICTM Conference 2017: Production technology for turbomachine manufacturing of the future

16.11.2016 | Event News

Innovation Day Laser Technology – Laser Additive Manufacturing

01.11.2016 | Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

 
Latest News

Robot on demand: Mobile machining of aircraft components with high precision

06.12.2016 | Power and Electrical Engineering

A new dead zone in the Indian Ocean could impact future marine nutrient balance

06.12.2016 | Earth Sciences

Significantly more productivity in USP lasers

06.12.2016 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>