Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Death dance reveals secrets of apoptosis in dissociated human ES cells

09.08.2010
Researchers at the RIKEN Center for Developmental Biology have unraveled the mystery of why human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells undergo programmed cell death (apoptosis) when cultured in isolation.

By unlocking the potential of cell therapy techniques, the discovery promises new hope to sufferers of debilitating degenerative diseases.

Cell dissociation, a technique for isolating cells in procedures such as subcloning, poses one of the greatest obstacles to effective stem cell research due to its damaging effects on human ES cells. 99% of human ES cells cultured in this way are destroyed by an extensive apoptotic response that is curiously absent in mouse ES cells. Earlier research by the researchers uncovered that inhibition of a protein known as the Rho-associated kinase (ROCK) reduced this rate of cell death by 30%, yet fundamental questions remained about the mechanisms involved.

To answer these questions, the researchers applied live-cell imaging to the earliest phase of dissociation in human and mouse ES cells. Results revealed a striking contrast: whereas the mouse ES cells hardly moved, the human ES cells skittered about in a so-called “death dance”, immediately sprouting finger-shaped bulges, known as blebs, which grew until the cells burst and died. The researchers traced this early-onset blebbing, whose duration and severity exceeded anything ever before observed, to the hyperactivation of myosin, a type of protein responsible for cell motility.

Contrary to expectation, the researchers went on demonstrate that it is this myosin hyperactivation, mediated by activation of the ROCK kinase, which is the direct cause of apoptosis in dissociated human ES cells, and not the blebbing itself. Further implicated in this process is the inhibition of another protein known for its role in cell motility, Rac, which together with ROCK activation strongly promotes myosin hyperactivation leading to cell death.

Reported in Cell Stem Cell, these results provide a first ever comprehensive elucidation of mechanisms underlying dissociation-induced apoptosis in human ES cells, pointing the way to safer and more effective cellular therapy treatments for a range of debilitating degenerative diseases.

For more information, please contact:

Dr. Yoshiki Sasai
Laboratory for Organogenesis and Neurogenesis
RIKEN Center for Developmental Biology
Tel: +81-(0)78-306-1841 / Fax: +81-(0)78-306-1854
Ms. Tomoko Ikawa (PI officer)
Global Relations Office
RIKEN
Tel: +81-(0)48-462-1225 / Fax: +81-(0)48-463-3687
Email: koho@riken.jp
Journal information
1. Ohgushi et al., Molecular Pathway and Cell State Responsible for Dissociation-Induced Apoptosis in Human Pluripotent Stem Cells, Cell Stem Cell (2010), doi:10.1016/j.stem.2010.06.018

gro-pr | Research asia research news
Further information:
http://www.riken.jp
http://www.researchsea.com

More articles from Life Sciences:

nachricht Fine organic particles in the atmosphere are more often solid glass beads than liquid oil droplets
21.04.2017 | Max-Planck-Institut für Chemie

nachricht Study overturns seminal research about the developing nervous system
21.04.2017 | University of California - Los Angeles Health Sciences

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Deep inside Galaxy M87

The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.

Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...

Im Focus: A Quantum Low Pass for Photons

Physicists in Garching observe novel quantum effect that limits the number of emitted photons.

The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...

Im Focus: Microprocessors based on a layer of just three atoms

Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.

Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...

Im Focus: Quantum-physical Model System

Computer-assisted methods aid Heidelberg physicists in reproducing experiment with ultracold atoms

Two researchers at Heidelberg University have developed a model system that enables a better understanding of the processes in a quantum-physical experiment...

Im Focus: Glacier bacteria’s contribution to carbon cycling

Glaciers might seem rather inhospitable environments. However, they are home to a diverse and vibrant microbial community. It’s becoming increasingly clear that they play a bigger role in the carbon cycle than previously thought.

A new study, now published in the journal Nature Geoscience, shows how microbial communities in melting glaciers contribute to the Earth’s carbon cycle, a...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Expert meeting “Health Business Connect” will connect international medical technology companies

20.04.2017 | Event News

Wenn der Computer das Gehirn austrickst

18.04.2017 | Event News

7th International Conference on Crystalline Silicon Photovoltaics in Freiburg on April 3-5, 2017

03.04.2017 | Event News

 
Latest News

New quantum liquid crystals may play role in future of computers

21.04.2017 | Physics and Astronomy

A promising target for kidney fibrosis

21.04.2017 | Health and Medicine

Light rays from a supernova bent by the curvature of space-time around a galaxy

21.04.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>