Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Death dance reveals secrets of apoptosis in dissociated human ES cells

09.08.2010
Researchers at the RIKEN Center for Developmental Biology have unraveled the mystery of why human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells undergo programmed cell death (apoptosis) when cultured in isolation.

By unlocking the potential of cell therapy techniques, the discovery promises new hope to sufferers of debilitating degenerative diseases.

Cell dissociation, a technique for isolating cells in procedures such as subcloning, poses one of the greatest obstacles to effective stem cell research due to its damaging effects on human ES cells. 99% of human ES cells cultured in this way are destroyed by an extensive apoptotic response that is curiously absent in mouse ES cells. Earlier research by the researchers uncovered that inhibition of a protein known as the Rho-associated kinase (ROCK) reduced this rate of cell death by 30%, yet fundamental questions remained about the mechanisms involved.

To answer these questions, the researchers applied live-cell imaging to the earliest phase of dissociation in human and mouse ES cells. Results revealed a striking contrast: whereas the mouse ES cells hardly moved, the human ES cells skittered about in a so-called “death dance”, immediately sprouting finger-shaped bulges, known as blebs, which grew until the cells burst and died. The researchers traced this early-onset blebbing, whose duration and severity exceeded anything ever before observed, to the hyperactivation of myosin, a type of protein responsible for cell motility.

Contrary to expectation, the researchers went on demonstrate that it is this myosin hyperactivation, mediated by activation of the ROCK kinase, which is the direct cause of apoptosis in dissociated human ES cells, and not the blebbing itself. Further implicated in this process is the inhibition of another protein known for its role in cell motility, Rac, which together with ROCK activation strongly promotes myosin hyperactivation leading to cell death.

Reported in Cell Stem Cell, these results provide a first ever comprehensive elucidation of mechanisms underlying dissociation-induced apoptosis in human ES cells, pointing the way to safer and more effective cellular therapy treatments for a range of debilitating degenerative diseases.

For more information, please contact:

Dr. Yoshiki Sasai
Laboratory for Organogenesis and Neurogenesis
RIKEN Center for Developmental Biology
Tel: +81-(0)78-306-1841 / Fax: +81-(0)78-306-1854
Ms. Tomoko Ikawa (PI officer)
Global Relations Office
RIKEN
Tel: +81-(0)48-462-1225 / Fax: +81-(0)48-463-3687
Email: koho@riken.jp
Journal information
1. Ohgushi et al., Molecular Pathway and Cell State Responsible for Dissociation-Induced Apoptosis in Human Pluripotent Stem Cells, Cell Stem Cell (2010), doi:10.1016/j.stem.2010.06.018

gro-pr | Research asia research news
Further information:
http://www.riken.jp
http://www.researchsea.com

More articles from Life Sciences:

nachricht Water forms 'spine of hydration' around DNA, group finds
26.05.2017 | Cornell University

nachricht How herpesviruses win the footrace against the immune system
26.05.2017 | Helmholtz-Zentrum für Infektionsforschung

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Can the immune system be boosted against Staphylococcus aureus by delivery of messenger RNA?

Staphylococcus aureus is a feared pathogen (MRSA, multi-resistant S. aureus) due to frequent resistances against many antibiotics, especially in hospital infections. Researchers at the Paul-Ehrlich-Institut have identified immunological processes that prevent a successful immune response directed against the pathogenic agent. The delivery of bacterial proteins with RNA adjuvant or messenger RNA (mRNA) into immune cells allows the re-direction of the immune response towards an active defense against S. aureus. This could be of significant importance for the development of an effective vaccine. PLOS Pathogens has published these research results online on 25 May 2017.

Staphylococcus aureus (S. aureus) is a bacterium that colonizes by far more than half of the skin and the mucosa of adults, usually without causing infections....

Im Focus: A quantum walk of photons

Physicists from the University of Würzburg are capable of generating identical looking single light particles at the push of a button. Two new studies now demonstrate the potential this method holds.

The quantum computer has fuelled the imagination of scientists for decades: It is based on fundamentally different phenomena than a conventional computer....

Im Focus: Turmoil in sluggish electrons’ existence

An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.

We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...

Im Focus: Wafer-thin Magnetic Materials Developed for Future Quantum Technologies

Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.

Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...

Im Focus: World's thinnest hologram paves path to new 3-D world

Nano-hologram paves way for integration of 3-D holography into everyday electronics

An Australian-Chinese research team has created the world's thinnest hologram, paving the way towards the integration of 3D holography into everyday...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Marine Conservation: IASS Contributes to UN Ocean Conference in New York on 5-9 June

24.05.2017 | Event News

AWK Aachen Machine Tool Colloquium 2017: Internet of Production for Agile Enterprises

23.05.2017 | Event News

Dortmund MST Conference presents Individualized Healthcare Solutions with micro and nanotechnology

22.05.2017 | Event News

 
Latest News

How herpesviruses win the footrace against the immune system

26.05.2017 | Life Sciences

Water forms 'spine of hydration' around DNA, group finds

26.05.2017 | Life Sciences

First Juno science results supported by University of Leicester's Jupiter 'forecast'

26.05.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>