Lignin is the double-edged sword of biofuels: if you are making cellulosic ethanol, you want less lignin because it blocks the breakdown of cellulose. If you are using pyrolytic methods, you want more lignin because lignin contains more energy than cellulose.
Whether you wish to maximize or minimize lignin content, an understanding of lignin synthesis is essential and has proved elusive. Lignin is a key adaptation to life on land, as it strengthens plant cell walls thereby helping land plants stand upright and reinforcing the cell walls of the specialized water-conducting tubes that are another key adaptation to growth in terrestrial environments.
The lignin polymer is made up of a complex arrangement of subunits and its subunit composition differs among different species. For example, ferns and conifers have lignin composed mainly of p-hydroxyphenyl (H) and guaiacyl (G) lignin units. Flowering plants have H and G subunits, plus syringyl (S) subunits derived from sinapyl alcohol. Interestingly, S lignin is also found in some lycophytes, including the spikemoss Selaginella (photo). In research published this week in The Plant Cell, a team of researchers led by Clint Chapple of Purdue University showed that lignin synthesis proceeds along a different path in Selaginella. Their work centers on the characterization of the enzyme ferulate 5-hydroxylase (F5H); in flowering plants, this enzyme produces S lignin units from G lignin precursors. By comparing the Selaginella enzyme (Sm F5H) to the F5H from the model flowering plant Arabidopsis thaliana (At F5H), the authors found that Sm F5H could both catalyze the same reaction as At F5H and could also catalyze an additional reaction, acting on precursors of H lignin to form precursors to G and S lignin, and thereby bypassing four steps in angiosperm lignin synthesis. Indeed, transgenic expression of Sm F5H can restore normal lignin deposition to Arabidopsis plants with mutations in other enzymes of lignin biosynthesis. Interestingly, some combinations of transgenic Sm F5H and Arabidopsis lignin mutations produce lignin compositions likely not seen in nature, indicating that manipulation of this pathway can be used to engineer lignin composition. Moreover, since different lignin subunit compositions produce different lignin structural properties, this engineering may affect biomass characteristics such as digestibility. Author Clinton Chapple notes “It is exciting to realize that the study of plants so distantly related to crops can provide us with new tools to engineer plants that are of benefit to humans.”
This research also provides interesting insights on convergent evolution, the process whereby different evolutionary lineages arrive at similar adaptations, such as the independent evolution of wings for flight in bats and birds. Selaginella is part of one of the oldest divisions of vascular plants, resulting from an ancient split between the lycophytes and euphyllophytes (which include all modern seed plants). Similar to bat wings and bird wings, the synthesis of S lignin appears to have arisen independently in flowering plants and in lycophytes. Thus, this research provides both an interesting window on convergent evolution in plants and a potentially useful tool for engineering lignin synthesis.
This research was supported by the National Science Foundation, the U.S. Department of Energy office of Science, and the Life Sciences Research Foundation.
Jennifer Mach | EurekAlert!
Cryo-electron microscopy achieves unprecedented resolution using new computational methods
24.03.2017 | DOE/Lawrence Berkeley National Laboratory
How cheetahs stay fit and healthy
24.03.2017 | Forschungsverbund Berlin e.V.
Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...
In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...
20.03.2017 | Event News
14.03.2017 | Event News
07.03.2017 | Event News
24.03.2017 | Materials Sciences
24.03.2017 | Physics and Astronomy
24.03.2017 | Physics and Astronomy