However, these networks have surprising functions. Only a few years ago researchers from the Helmholtz Centre for Environmental Research (UFZ) discovered that bacteria travel over the fungal threads through the labyrinth of soil pores, much the same as on a highway.
Micrograph of the mycelia-forming soil microorganism Pythium ultimum transporting the contaminant phenanthrene in its hyphae: Overlay of a light transmission and a fluorescence micrograph of Pythium ultimum. The hyphae contain phenanthrene-enriched vesicles that fluoresce in blue (bar length: 5 micrometers).
Photo: Susan Foß/UFZ
Now, together with British colleagues from the University of Lancaster, the UFZ researchers have come upon another phenomenon. Accordingly, the fungal networks also transports contaminants which are otherwise largely immobile in the ground. These living pipelines can therefore contribute to the restoration of impacted areas, write the researchers in the journal Environmental Science & Technology.
Some bacteria develop a voracious appetite for contaminants. They nourish themselves from toxic chemicals and at the same time convert these to harmless substances. This makes them valuable allies for the elimination of different forms of environmental pollution. Certain soil microbes, for example, are fully capable of restoring impacted ground by natural means. Theoretically at least. In practice, however, the tiny helpers are frequently pushed to their limits. "The problem is that the contaminants often do not even reach them", explains UFZ researcher Lukas Y. Wick, in charge of the new study.
The bacteria frequently have difficulties with substances which are largely insoluble in water. These include, for example, the polycyclic aromatic hydrocarbons (PAHs) found in crude oil and coal, released in practically all combustion processes. Along well-travelled roads, in the vicinity of airports or old gas works sites, the ground can also be polluted with these compounds. As many PAHs are considered to be carcinogenic, the help of bacteria for the degradation of this pollution would be entirely welcome.
In the complicated labyrinth of water-filled and air-filled pores found throughout the soil, however, bacteria and PAHs rarely come together. This is because the microorganisms are predominantly found in water and in thin liquid films. "The PAHs are virtually insoluble in water, so that they frequently staple themselves to particles of ground in tiny, air-filled pores", which the bacteria are unable to reach, explains Lukas Y. Wick. Between them and their sources of nutrition there are air barriers.
A living network
Nevertheless, there are soil organisms which can effectively overcome such barriers. These include fungi and a group of similar organisms which biologists describe as "pseudo-fungi". Both can grow either in water or in air and are found throughout the soil with a network of fine threads. Each of these so-called hyphae is in fact only a few thousandths of a millimetre thick. Yet, together they form a network of enormous dimensions. One single gram of soil may contain up to 1000 to 10,000 meters of fungal threads. A single braid thereby can extend over several square kilometres. Fungi are therefore in fact the largest living creatures on the Earth today.
That bacteria are fully capable of utilising the infrastructure of this huge neighbour, emerges from an earlier study of Lukas Y. Wick and his colleagues. The fungal braiding appears to be a kind of highway over which the microorganisms can effectively travel and propagate. They move on the surface of the hyphae and, in this way, overcome the air barriers between two water-filled pores without problem.
But what if the fungal network offers good travel possibilities not only for bacteria, but for contaminants as well? Nevertheless, it is known that the transport of nutrients which the fungus requires to survive takes place within the hyphae. Why should this not function, then, with other substances? Together with colleagues from the University of Lancaster, the UFZ researchers have now pursued this question.
For their investigations the researchers employed a pseudo-fungus with the name Pythium ultimum, which is widespread in the soil. This was placed on a central plate with nutrients, proceeding from which it could extend its hyphae to the right and left to two further sources of nourishment. The three nourishment stations were connected by rectangles of nourishment-free material. However, there were several gaps between the nourishment plates and rectangles containing only air. These were intended to simulate the air-filled pores in the ground.
At the edge of a rectangle the UFZ researchers applied a polycyclic aromatic carbohydrate with the name Phenanthrene. They then investigated at regular intervals whether this substance could be detected in other areas of the test path. "The results were astounding", says Lukas Y. Wick. Within a few hours the carbohydrate had migrated from one end of the experimental arrangement to the other – from ten to one hundred times faster than it could have by simple diffusion. Furthermore, it overcame the air gaps with no difficulty, which was not possible over the same path without hyphal networks. "The hyphal networks are therefore not only highways for bacteria, but also pipelines for contaminants", concludes Lukas Wick. "Per hour a single hypha can transport up to 600 times the weight of an individual bacterium".
With a special microscope of the British colleagues it was even possible to observe this transport in detail. Accordingly, the contaminant migrates through the cell wall into the interior of the hyphae. There it is enclosed in tiny bubbles, which Pythium ultimum then actively pumps through its far-reaching network.
Contaminants in motion
In this way the fungal pipeline mobilises not only Phenanthrene, but also other substances virtually insoluble in water and therefore sooner immobile substances. The researchers repeated the experiment with a number of different PAHs, and all were found to be efficiently transported. However, over longer paths the transport of small molecules functioned better than that of large molecules. "Presumably the latter are not taken up as well by the hyphae", in the opinion of Lukas Y. Wick.
In this experiment even the longer paths consisted of only a few centimetres. This may appear to be relatively little, however it could still prove decisive for better contact between the contaminants and their decomposers, as the fungal pipeline effortlessly overcomes the minute air barriers between the water-filled soil pores.The researchers hope that this effect can be utilised in future for the restoration of impacted ground. The targeted use of fungal networks could accelerate the degradation of PAHs and perhaps also of other substances virtually insoluble in water. "But this may function only when one combines the right fungi and bacteria", explains Lukas Y. Wick. Some types of these organisms are simply not compatible or even mutually inhibit each other. Consequently, the UFZ researchers are now searching for the most suitable partners for their microbial contaminant eliminator team.
Shoko Furuno, Susan Foss, Ed Wild, Kevin C. Jones, Kirk T. Semple, Hauke Harms and Lukas Y. Wick (2012): Mycelia Promote Active Transport and Spatial Dispersion of Polycyclic Aromatic Hydrocarbons. Environ. Sci. Technol. 2012, 46, 5463−5470
The studies were founded by the EU project MC-20 984 EST (RAISEBIO) and by the Helmholtz Association ("CITE - Chemicals in the Environment").
Further InformationHelmholtz Centre for Environmental Research (UFZ)
At the Helmholtz Centre for Environmental Research (UFZ) scientists are researching the causes and consequences of far-reaching changes to the environment. They are concerned with water resources, biological diversity, the consequences of climate change and adaptability, environmental and biotechnologies, bioenergy, the behaviour of chemicals in the environment, their effect on health, modelling and social science issues. Their guiding theme: Our research contributes to the sustainable use of natural resources and helps to secure this basis for life over the long term under the effects of global change. The UFZ employs 1,000 people in Leipzig, Halle and Magdeburg. It is financed by the federal government and the federal states of Saxony and Saxony-Anhalt.
The Helmholtz Association contributes towards solving major and pressing social, scientific and economic issues with scientific excellence in six research areas: Energy, Earth and Environment, Health, Key Technologies, Structure of Matter, Aeronautics, Aerospace and Transport. The Helmholtz Association is Germany’s largest scientific organisation with over 33,000 employees in 18 research centres and an annual budget of approximately 3.4 billion euros. Its work stands in the tradition of the naturalist Hermann von Helmholtz (1821-1894).
Tilo Arnhold | EurekAlert!
Newly designed molecule binds nitrogen
23.02.2018 | Julius-Maximilians-Universität Würzburg
Atomic Design by Water
23.02.2018 | Max-Planck-Institut für Eisenforschung GmbH
A newly developed laser technology has enabled physicists in the Laboratory for Attosecond Physics (jointly run by LMU Munich and the Max Planck Institute of Quantum Optics) to generate attosecond bursts of high-energy photons of unprecedented intensity. This has made it possible to observe the interaction of multiple photons in a single such pulse with electrons in the inner orbital shell of an atom.
In order to observe the ultrafast electron motion in the inner shells of atoms with short light pulses, the pulses must not only be ultrashort, but very...
A group of researchers led by Andrea Cavalleri at the Max Planck Institute for Structure and Dynamics of Matter (MPSD) in Hamburg has demonstrated a new method enabling precise measurements of the interatomic forces that hold crystalline solids together. The paper Probing the Interatomic Potential of Solids by Strong-Field Nonlinear Phononics, published online in Nature, explains how a terahertz-frequency laser pulse can drive very large deformations of the crystal.
By measuring the highly unusual atomic trajectories under extreme electromagnetic transients, the MPSD group could reconstruct how rigid the atomic bonds are...
Quantum computers may one day solve algorithmic problems which even the biggest supercomputers today can’t manage. But how do you test a quantum computer to...
For the first time, a team of researchers at the Max-Planck Institute (MPI) for Polymer Research in Mainz, Germany, has succeeded in making an integrated circuit (IC) from just a monolayer of a semiconducting polymer via a bottom-up, self-assembly approach.
In the self-assembly process, the semiconducting polymer arranges itself into an ordered monolayer in a transistor. The transistors are binary switches used...
Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale
Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...
15.02.2018 | Event News
13.02.2018 | Event News
12.02.2018 | Event News
23.02.2018 | Physics and Astronomy
23.02.2018 | Health and Medicine
23.02.2018 | Physics and Astronomy