Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Cells on film

27.02.2012
Development of polymer film loaded with antibodies that can capture tumor cells shows promise as a diagnostic tool
Cancer cells that break free from a tumor and circulate through the bloodstream spread cancer to other parts of the body. But this process, called metastasis, is extremely difficult to monitor because the circulating tumor cells (CTCs) can account for as few as one in every billion blood cells.

Research led by scientists at the RIKEN Advanced Science Institute in Wako, in collaboration with colleagues at the University of California, Los Angeles, and the Institute of Chemistry at the Chinese Academy of Sciences, Beijing, has produced a polymer film that can capture specific CTCs1. With further development, the system could help doctors to diagnose an advancing cancer and assess the effectiveness of treatments.

The researchers used a small electrical voltage to help deposit a conducting polymer film of poly(3,4-ethylenedioxythiophene) (PEDOT) bearing carboxylic acid groups on to a 2-centimeter-square glass base (Fig. 1). The polymer formed nanodots, tiny bumps that measure 100 to 300 nanometers across, depending on the voltage used (1–1.4 V).
Adding a chemical linker to the film allowed it to bind a protein called streptavidin; this protein then joined to an antibody. In turn, the antibody could latch on to an antigen called epithelial cell adhesion molecule (EpCAM), which is produced by most tumor cells. In this way, the film could grab tumor cells from just a few milliliters of a blood sample.

The team tested several types of tumor cells on films with various sizes and densities of nanodots, and used a microscope to observe how well they captured the cells. The most effective film, with nanodots measuring about 230 nanometers across and containing about 8 dots per square micrometer, captured roughly 240 breast-cancer cells per square millimeter of film. In contrast, it caught fewer than 30 cervical cancer cells that do not express EpCAM, proving that the antibody used on the film is highly selective. A smooth PEDOT-carboxylic acid film with the same antibody captured only 50 or so breast cancer cells.

The film’s efficiency depends on the size and spacing of the nanodots, and the presence of the capturing antibody. Since these can be easily modified, the same method could be used to make films that sense other types of cells.

The next step is to “further optimize the nanostructures of the conducting polymers and understand in more detail the cell-capturing mechanism,” says RIKEN unit leader Hsiao-hua Yu. “We are also currently working on a direct electrical readout of the captured cells, without needing to use a microscope.”

The corresponding author for this highlight is based at the Yu Initiative Research Unit, RIKEN Advanced Science Institute

gro-pr | Research asia research news
Further information:
http://www.riken.jp
http://www.researchsea.com

More articles from Life Sciences:

nachricht Cryo-electron microscopy achieves unprecedented resolution using new computational methods
24.03.2017 | DOE/Lawrence Berkeley National Laboratory

nachricht How cheetahs stay fit and healthy
24.03.2017 | Forschungsverbund Berlin e.V.

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Argon is not the 'dope' for metallic hydrogen

24.03.2017 | Materials Sciences

Astronomers find unexpected, dust-obscured star formation in distant galaxy

24.03.2017 | Physics and Astronomy

Gravitational wave kicks monster black hole out of galactic core

24.03.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>