The chemical is hydrogen peroxide (H2O2), the active ingredient in color safe bleach. Produced in all animal cells, hydrogen peroxide may act as a signal for the active and resting phases of living things, new research by USC biologists suggests.
A study published in the journal PLoS ONE shows that hydrogen peroxide given to fruit flies has dramatic effects on their daily rhythms and activity levels.
“H2O2 might be functioning as a systemic signal by which rhythms are regulated within cells and between cells,” said lead author John Tower, associate professor in molecular and computational biology at the USC College of Letters, Arts and Sciences.
Most people are familiar with the concept of a circadian rhythm that governs sleeping and waking. But that is not the only circadian rhythm in the body.
Many organs and tissues within the body have their own independent circadian rhythms, and they also interact to coordinate their rhythms.
Tower’s study suggests a link between metabolism – the production of energy by mitochondria, often described as the energy factories inside cells – and an animal’s daily rhythms.
Mitochondria produce hydrogen peroxide as a by-product of oxygen combustion, making the chemical a candidate signal molecule.
“This is a logical way to connect rhythms to metabolism,” Tower said.
“We know a lot about how circadian rhythms are regulated within certain cells. However, we have very little information on what signals coordinate circadian rhythms and how these rhythms are linked between metabolism and behavior.”
For the rhythms of even two cells to agree, some sort of signal has to pass between them.
Tower’s research group set out to find the signal by probing the action of an enzyme in mitochondria that converts toxic by-products of the body’s combustion process into hydrogen peroxide, itself a harmful but less toxic substance which other defenses later break down further.
Tower and his team had noticed that overexpression of the enzyme, known as superoxide dismutase (SOD), boosted the activity level of fruit flies and even increased the life span of certain genetically engineered strains.
Tower suspected that hydrogen peroxide was the key ingredient in SOD’s action.
“Hydrogen peroxide is a great candidate for a signaling molecule that would be involved in rhythms and behaviors. It’s the most stable and diffusible of the reactive oxygen species (by-products of combustion), but no one had demonstrated a role for it.”
As a test, Tower’s group administered hydrogen peroxide directly to fruit flies through feeding and injection.
The researchers observed similar effects from the direct administration of hydrogen peroxide and the over-expression of the SOD enzyme.
Both strategies increased the activity levels of adult flies. Long-term direct treatment with hydrogen peroxide suppressed daily rhythms, while SOD over-expression altered those rhythms.
Tower explained that he had not expected identical results from direct treatment versus genetic over-expression.
“I think it’s just a little too crude of an intervention, to feed them or inject them with the drug,” he said, because those effects will not be rhythmic, whereas production of hydrogen peroxide by the mitochondria and by SOD is expected to be rhythmic and to correspond to the rhythm of metabolism.
Still, the similarities in the flies’ reactions to direct treatment and to SOD over-expression suggested to the researchers that hydrogen peroxide is the crucial chemical.
“It’s a very exciting result for us that our data now start to point to hydrogen peroxide as perhaps being a relevant signaling molecule for coupling metabolism to behaviors and rhythms in the animal,” Tower said.
Hydrogen peroxide would govern rhythms inside each cell as well as between cells, Tower added.
Every cell alternates between a metabolic phase – in which it burns oxygen to make energy – and a detoxification phase in which the cell breaks down the harmful by-products of combustion.
Those rhythms must be coupled with the energy-producing activity of the mitochondria.
“Because hydrogen peroxide is produced by mitochondria as a product of metabolism, it’s a great candidate for a relevant signal that might be modulating these cellular rhythms,” Tower said.
Tower’s group was able to correlate fly activity and hydrogen peroxide concentration precisely through a unique three-dimensional movement tracking system developed by first author and doctoral student Dhruv Grover (http://uscnews2.usc.edu/newstools/detail.php?recordnum=16087).
Doctoral students Daniel Ford, Nicholas Hoe and Aysen Erdem and undergraduate Christopher Brown also contributed to the study. Simon Tavare, professor of mathematics and molecular and computational biology at USC College, designed and supervised the statistical analyses.Read the study at
Carl Marziali | Newswise Science News
The birth of a new protein
20.10.2017 | University of Arizona
Building New Moss Factories
20.10.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau
University of Maryland researchers contribute to historic detection of gravitational waves and light created by event
On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...
Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.
Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....
Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).
When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...
Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.
How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...
Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.
It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...
17.10.2017 | Event News
10.10.2017 | Event News
10.10.2017 | Event News
20.10.2017 | Information Technology
20.10.2017 | Materials Sciences
20.10.2017 | Interdisciplinary Research